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Bioanalysis Volume 5, 2013 - Issue 22
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Research Article

Mobile Phase Ph and Organic Modifier in Reversed-Phase LC–ESI-MS Bioanalytical Methods: Assessment of Sensitivity, Chromatography and Correlation of Retention Time with In Silico Logd Predictions

Steve SilvesterPre-clinical Bioanalysis & Toxicokinetics, Drug Safety & Metabolism, AstraZeneca R&D Alderley, Macclesfield, Cheshire, SK10 4TG, UK. [email protected]
Pages 2753-2770 | Published online: 21 Nov 2013

References

  • Trufelli H , PalmaP, FamigliniG, CappielloA. An overview of matrix effects in liquid chromatography–mass spectrometry. Mass Spectrom. Rev. 30, 491–509 (2011).
  • Antignac JP , WashF, MonteauHD, BrabanderFA, BizetBL. The ion suppression phenomenon in liquid chromatography–mass spectrometry and its consequences in the field of residue analysis. Anal. Chim. Acta529, 129–136 (2005).
  • Côté C , BergeronA, Mess J-N, Furtado M, Garofolo F. Matrix effect elimination during LC–MS/MS bioanalytical method development. Bioanalysis1(7), 1243–1257 (2009).
  • Kostiainen R , KauppilaTJ. Effect of eluent on the ionization process in liquid chromatography–mass spectrometry. J. Chromatogr. A1216, 685–699 (2009).
  • Gao S , Zhang Z-P, Karnes HT. Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives. J. Chromatogr. B825, 98–110 (2005).
  • Sillén H , MagnellN. Screening ionization and chromatography conditions for quantitative LC–MS methods. J. Chromatogr. B877, 3581–3588 (2009).
  • Mess J-N . Lahaie M, Furtado M, Garofolo F. Effect of high pH mobile phase on the sensitivity of multiple drugs by LC positive electrospray ionization MS/MS. Bioanalysis1(8), 1419–1430 (2009).
  • Rainville PD , SmithNW, CowanD, PlumbRS. Comprehensive investigation of the influence of acidic, basic and organic mobile phase compositions on bioanalytical assay sensitivity in positive ESI mode LC–MS/MS. J. Pharm. Biomed. Anal. 59, 138–150 (2012).
  • Peng L , FarkasT. Analysis of basic compounds by reversed-phase liquid chromatography–electrospray mass spectrometry in high-pH mobile phases. J. Chromatogr. A1179, 131–144 (2008).
  • Cheng Y-F . Lu Z, Neue U. Ultrafast liquid chromatography/ultraviolet and liquid chromatography–tandem mass spectrometric analysis. Rapid Commun. Mass Spectrom. 15, 141–151 (2001).
  • Delatour C , LeclercqL. Letter to the editor. Rapid Commun. Mass Spectrom. 19, 1359–1362 (2005).
  • Mallet CR , LuZ, MazzeoJR. A study of ion suppression effects in electrospray ionization from mobile phase additives and solid phase extracts. Rapid Commun. Mass Spectrom. 18, 49–58 (2004).
  • Meng M , WangL, VoelkerT, ReuschelS, Van Horne KC, Bennett P. A systematic approach for developing a robust LC–MS/MS method for bioanalysis. Bioanalysis5(1), 91–115 (2013).
  • Mulvana DE . Critical topics in ensuring data quality in bioanalytical LC–MS method development. Bioanalysis2(6), 1051–1072 (2010).
  • Silvester S , SmithL. Profiling phospholipid elution in reversed-phase LC–MS/MS bioanalytical methods in order to avoid matrix effects. Bioanalysis4(8), 879–895 (2012).
  • Avdeef A . Physicochemical profiling (solubility, permeability and charge state). Curr. Top. Med. Chem. 1, 277–351 (2001).
  • Giaginis C , Tsantili-KakoulidouA. Current state of the art in HPLC methodology for lipophilicity assessment of basic drugs. A review. J. Liq. Chromatogr. Relat. Technol. 31, 79–96 (2008).
  • Nasal A , SilukD, KaliszanR. Chromatographic retention parameters in medicinal chemistry and molecular pharmacology. Curr. Med. Chem. 10, 381–426 (2003).
  • Zhang G , WujcikCE. Overcoming ionization effects through chromatography: A case study for the ESI-LC–MS/MS quantitation of a hydrophobic therapeutic agent in human serum using a stable-label internal standard. J. Chromatogr. B877, 2003–2010 (2009).
  • Kadar EP , WujcikCE, WolfordDP, KavetskaiaO. Rapid determination of the applicability of hydrophilic interaction chromatography utilizing ACD Labs LogD suite: a bioanalytical application. J. Chromatogr. B863, 1–8 (2008).
  • McCalley DV . Comparison of peak shapes obtained with volatile (mass spectrometry-compatible) buffers and conventional buffers in reversed-phase high-performance liquid chromatography of bases on particulate and monolithic columns. J. Chromatogr. A987, 17–28 (2003).
  • Subirats X , BoschE, RosésM. Buffer considerations for LC and LC–MS. LC GC N. Am. Nov 1, 2009.
  • Canals I , OumadaFZ, RosésM, BoschE. Retention of ionizable compounds on HPLC. 6. pH measurements with the glass electrode in methanol–water mixtures. J. Chromatogr. A911, 191–202 (2003).
  • Espinosa S , BoschE, RosésM. Retention of ionizable compounds on HPLC. 12. The properties of liquid chromatography buffers in acetonitrile–water mobile phases that influence HPLC retention. Anal. Chem. 74, 3809–3818 (2002).
  • Kebarle P , VerkerkUH. Electrospray: from ions in solution to ions in the gas phase, what we know now. Mass Spectrom. Rev. 28, 898–917 (2009).
  • McCalley DV . The challenges of the analysis of basic compounds by high performance liquid chromatography: some possible approaches for improved separations. J. Chromatogr. A1217, 858–880 (2010).
  • Neue UD , PhoebeCH, TranK, Cheng Y-F, Lu Z. Dependence of reversed-phase retention of ionizable analytes on pH, concentration of organic solvent and silanol activity. J. Chromatogr. A925, 49–67 (2001).
  • Méndez A , BoschE, RosésM, NeueUD. Comparison of the acidity of residual silanol groups in several liquid chromatography columns. J. Chromatogr. A986, 33–44 (2003).
  • Davies NH , EuerbyMR, McCalleyDV. Analysis of basic compounds by reversed-phase high performance liquid chromatography using hybrid inorganic/organic phases at high pH. J. Chromatogr. A1178, 71–78 (2008).
  • Hupp AM , McGuffinVL. Thermodynamic and kinetic characterization of a bridged-ethylene hybrid C18 stationary phase. J. Chromatogr. A1217, 6241–6249 (2010).
  • ACD/LogD Suite Version 12.0 Reference Manual. Advanced Chemistry Development, Inc., Copyright © 1994–2008.
  • Snyder LR , DolanJW. High-Performance Gradient Elution. The Practical Application of the Linear-Solvent-Strength Model. John Wiley & Sons, Inc., NJ, USA (2007).
  • Meloun M , BordovskáS. Benchmarking and validating algorithms that estimate pKa values of drugs based on their molecular structures. Anal. Bioanal. Chem. 389, 1267–1281 (2007).
  • Andrés A , TéllezA, RosésM, BoschE. Chromatographic models to predict the elution of ionizable analytes by organic modifier gradient in reversed phase liquid chromatography. J. Chromatogr. A1247, 71–80 (2012).
  • Wiczling P , NasalA, KubikL, KaliszanR. A new pH/organic modifier gradient RP HPLC method for convenient determination of lipophilicity and acidity of drugs as applied to established imidazoline agents. Eur. J. Pharm. Sci. 47, 1–5 (2012).
  • Rainville PD , SimeoneJL, McCarthySM, SmithNW, CowanD, PlumbRS. Investigation of microbore UPLC and nontraditional mobile phase compositions for bioanalytical LC–MS/MS. Bioanalysis4(11), 1287–1297 (2012).

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