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Protocol

Detection of Saccharomyces cerevisiae Atg13 by western blot

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Pages 514-517 | Received 20 Nov 2013, Accepted 02 Jan 2014, Published online: 09 Jan 2014

References

  • Reggiori F, Klionsky DJ. Autophagic processes in yeast: mechanism, machinery and regulation. Genetics 2013; 194:341 - 61; http://dx.doi.org/10.1534/genetics.112.149013; PMID: 23733851
  • Chen Y, Klionsky DJ. The regulation of autophagy - unanswered questions. J Cell Sci 2011; 124:161 - 70; http://dx.doi.org/10.1242/jcs.064576; PMID: 21187343
  • Cheong H, Nair U, Geng J, Klionsky DJ. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae.. Mol Biol Cell 2008; 19:668 - 81; http://dx.doi.org/10.1091/mbc.E07-08-0826; PMID: 18077553
  • Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y. Tor-mediated induction of autophagy via an Apg1 protein kinase complex. J Cell Biol 2000; 150:1507 - 13; http://dx.doi.org/10.1083/jcb.150.6.1507; PMID: 10995454
  • Kamada Y, Yoshino K, Kondo C, Kawamata T, Oshiro N, Yonezawa K, Ohsumi Y. Tor directly controls the Atg1 kinase complex to regulate autophagy. Mol Cell Biol 2010; 30:1049 - 58; http://dx.doi.org/10.1128/MCB.01344-09; PMID: 19995911
  • Stephan JS, Yeh YY, Ramachandran V, Deminoff SJ, Herman PK. The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy. Proc Natl Acad Sci U S A 2009; 106:17049 - 54; http://dx.doi.org/10.1073/pnas.0903316106; PMID: 19805182
  • Kraft C, Kijanska M, Kalie E, Siergiejuk E, Lee SS, Semplicio G, Stoffel I, Brezovich A, Verma M, Hansmann I, et al. Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy. EMBO J 2012; 31:3691 - 703; http://dx.doi.org/10.1038/emboj.2012.225; PMID: 22885598
  • Deminoff SJ, Howard SC, Hester A, Warner S, Herman PK. Using substrate-binding variants of the cAMP-dependent protein kinase to identify novel targets and a kinase domain important for substrate interactions in Saccharomyces cerevisiae.. Genetics 2006; 173:1909 - 17; http://dx.doi.org/10.1534/genetics.106.059238; PMID: 16751660
  • Yeh YY, Wrasman K, Herman PK. Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae.. Genetics 2010; 185:871 - 82; http://dx.doi.org/10.1534/genetics.110.116566; PMID: 20439775
  • Scott SV, Nice DC III, Nau JJ, Weisman LS, Kamada Y, Keizer-Gunnink I, Funakoshi T, Veenhuis M, Ohsumi Y, Klionsky DJ. Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J Biol Chem 2000; 275:25840 - 9; http://dx.doi.org/10.1074/jbc.M002813200; PMID: 10837477
  • Cheong H, Klionsky DJ. Biochemical methods to monitor autophagy-related processes in yeast. Methods Enzymol 2008; 451:1 - 26; PMID: 19185709
  • Kabeya Y, Kamada Y, Baba M, Takikawa H, Sasaki M, Ohsumi Y. Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy. Mol Biol Cell 2005; 16:2544 - 53; http://dx.doi.org/10.1091/mbc.E04-08-0669; PMID: 15743910

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