CHO-gmt5, a novel CHO glycosylation mutant for producing afucosylated and asialylated recombinant antibodies

References

• Sondermann P, Huber R, Oosthuizen V, Jacob U. The 3.2-A crystal structure of the human IgG1 Fc fragment-Fc gammaRIII complex. Nature 2000; 406:267 - 73; http://dx.doi.org/10.1038/35018508; PMID: 10917521
   PubMed Web of Science ®Google Scholar
• Radaev S, Motyka S, Fridman WH, Sautes-Fridman C, Sun PD. The structure of a human type III Fcgamma receptor in complex with Fc. J Biol Chem 2001; 276:16469 - 77; http://dx.doi.org/10.1074/jbc.M100350200; PMID: 11297532
   PubMed Web of Science ®Google Scholar
• Krapp S, Mimura Y, Jefferis R, Huber R, Sondermann P. Structural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structural integrity. J Mol Biol 2003; 325:979 - 89; http://dx.doi.org/10.1016/S0022-2836(02)01250-0; PMID: 12527303
   PubMed Web of Science ®Google Scholar
• Shields RL, Lai J, Keck R, O’Connell LY, Hong K, Meng YG, et al. Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J Biol Chem 2002; 277:26733 - 40; http://dx.doi.org/10.1074/jbc.M202069200; PMID: 11986321
   PubMed Web of Science ®Google Scholar
• Shinkawa T, Nakamura K, Yamane N, Shoji-Hosaka E, Kanda Y, Sakurada M, et al. The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem 2003; 278:3466 - 73; http://dx.doi.org/10.1074/jbc.M210665200; PMID: 12427744
   PubMed Web of Science ®Google Scholar
• Okazaki A, Shoji-Hosaka E, Nakamura K, Wakitani M, Uchida K, Kakita S, et al. Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between IgG1 and FcgammaRIIIa. J Mol Biol 2004; 336:1239 - 49; http://dx.doi.org/10.1016/j.jmb.2004.01.007; PMID: 15037082
   PubMed Web of Science ®Google Scholar
• Matsumiya S, Yamaguchi Y, Saito J, Nagano M, Sasakawa H, Otaki S, et al. Structural comparison of fucosylated and nonfucosylated Fc fragments of human immunoglobulin G1. J Mol Biol 2007; 368:767 - 79; http://dx.doi.org/10.1016/j.jmb.2007.02.034; PMID: 17368483
   PubMed Web of Science ®Google Scholar
• Radaev S, Sun P. Recognition of immunoglobulins by Fcgamma receptors. Mol Immunol 2002; 38:1073 - 83; http://dx.doi.org/10.1016/S0161-5890(02)00036-6; PMID: 11955599
   PubMed Web of Science ®Google Scholar
• Niwa R, Shoji-Hosaka E, Sakurada M, Shinkawa T, Uchida K, Nakamura K, et al. Defucosylated chimeric anti-CC chemokine receptor 4 IgG1 with enhanced antibody-dependent cellular cytotoxicity shows potent therapeutic activity to T-cell leukemia and lymphoma. Cancer Res 2004; 64:2127 - 33; http://dx.doi.org/10.1158/0008-5472.CAN-03-2068; PMID: 15026353
   PubMed Web of Science ®Google Scholar
• Suzuki E, Niwa R, Saji S, Muta M, Hirose M, Iida S, et al. A nonfucosylated anti-HER2 antibody augments antibody-dependent cellular cytotoxicity in breast cancer patients. Clin Cancer Res 2007; 13:1875 - 82; http://dx.doi.org/10.1158/1078-0432.CCR-06-1335; PMID: 17363544
   PubMed Web of Science ®Google Scholar
• Cardarelli PM, Moldovan-Loomis MC, Preston B, Black A, Passmore D, Chen TH, et al. In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphoma. Clin Cancer Res 2009; 15:3376 - 83; http://dx.doi.org/10.1158/1078-0432.CCR-08-3222; PMID: 19401346
   PubMed Web of Science ®Google Scholar
• Junttila TT, Parsons K, Olsson C, Lu Y, Xin Y, Theriault J, et al. Superior in vivo efficacy of afucosylated trastuzumab in the treatment of HER2-amplified breast cancer. Cancer Res 2010; 70:4481 - 9; http://dx.doi.org/10.1158/0008-5472.CAN-09-3704; PMID: 20484044
   PubMed Web of Science ®Google Scholar
• Anthony RM, Nimmerjahn F, Ashline DJ, Reinhold VN, Paulson JC, Ravetch JV. Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc. Science 2008; 320:373 - 6; http://dx.doi.org/10.1126/science.1154315; PMID: 18420934
   PubMed Web of Science ®Google Scholar
• Kaneko Y, Nimmerjahn F, Ravetch JV. Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation. Science 2006; 313:670 - 3; http://dx.doi.org/10.1126/science.1129594; PMID: 16888140
   PubMed Web of Science ®Google Scholar
• Lim SF, Lee MM, Zhang P, Song Z. The Golgi CMP-sialic acid transporter: A new CHO mutant provides functional insights. Glycobiology 2008; 18:851 - 60; http://dx.doi.org/10.1093/glycob/cwn080; PMID: 18713811
   PubMed Web of Science ®Google Scholar
• Zhang P, Haryadi R, Chan KF, Teo G, Goh J, Pereira NA, et al. Identification of functional elements of the GDP-fucose transporter SLC35C1 using a novel Chinese hamster ovary mutant. Glycobiology 2012; 22:897 - 911; http://dx.doi.org/10.1093/glycob/cws064; PMID: 22492235
   PubMed Web of Science ®Google Scholar
• Chan KF, Zhang P, Song Z. Identification of essential amino acid residues in the hydrophilic loop regions of the CMP-sialic acid transporter and UDP-galactose transporter. Glycobiology 2010; 20:689 - 701; http://dx.doi.org/10.1093/glycob/cwq016; PMID: 20181793
   PubMed Web of Science ®Google Scholar
• Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell 2008; 31:294 - 301; http://dx.doi.org/10.1016/j.molcel.2008.06.016; PMID: 18657511
   PubMed Web of Science ®Google Scholar
• Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, et al. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 2005; 435:646 - 51; http://dx.doi.org/10.1038/nature03556; PMID: 15806097
   PubMed Web of Science ®Google Scholar
• Doyon Y, McCammon JM, Miller JC, Faraji F, Ngo C, Katibah GE, et al. Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol 2008; 26:702 - 8; http://dx.doi.org/10.1038/nbt1409; PMID: 18500334
   PubMed Web of Science ®Google Scholar
• Santiago Y, Chan E, Liu PQ, Orlando S, Zhang L, Urnov FD, et al. Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A 2008; 105:5809 - 14; http://dx.doi.org/10.1073/pnas.0800940105; PMID: 18359850
   PubMed Web of Science ®Google Scholar
• Carroll D, Morton JJ, Beumer KJ, Segal DJ. Design, construction and in vitro testing of zinc finger nucleases. Nat Protoc 2006; 1:1329 - 41; http://dx.doi.org/10.1038/nprot.2006.231; PMID: 17406419
   PubMed Web of Science ®Google Scholar
• Kim HJ, Lee HJ, Kim H, Cho SW, Kim JS. Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Genome Res 2009; 19:1279 - 88; http://dx.doi.org/10.1101/gr.089417.108; PMID: 19470664
   PubMed Web of Science ®Google Scholar
• Sander JD, Zaback P, Joung JK, Voytas DF, Dobbs D. Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res 2007; 35:Web Server issue W599-605; http://dx.doi.org/10.1093/nar/gkm349; PMID: 17526515
   PubMedGoogle Scholar
• Dreier B, Segal DJ, Barbas CF 3rd. Insights into the molecular recognition of the 5′-GNN-3′ family of DNA sequences by zinc finger domains. J Mol Biol 2000; 303:489 - 502; http://dx.doi.org/10.1006/jmbi.2000.4133; PMID: 11054286
   PubMed Web of Science ®Google Scholar
• Liu Q, Xia Z, Zhong X, Case CC. Validated zinc finger protein designs for all 16 GNN DNA triplet targets. J Biol Chem 2002; 277:3850 - 6; http://dx.doi.org/10.1074/jbc.M110669200; PMID: 11726671
   PubMed Web of Science ®Google Scholar
• Segal DJ, Dreier B, Beerli RR, Barbas CF 3rd. Toward controlling gene expression at will: selection and design of zinc finger domains recognizing each of the 5′-GNN-3′ DNA target sequences. Proc Natl Acad Sci U S A 1999; 96:2758 - 63; http://dx.doi.org/10.1073/pnas.96.6.2758; PMID: 10077584
   PubMed Web of Science ®Google Scholar
• Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases. Genome Res 2010; 20:81 - 9; http://dx.doi.org/10.1101/gr.099747.109; PMID: 19952142
   PubMed Web of Science ®Google Scholar
• Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, et al. An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol 2007; 25:778 - 85; http://dx.doi.org/10.1038/nbt1319; PMID: 17603475
   PubMed Web of Science ®Google Scholar
• Gaudray P, Trotter J, Wahl GM. Fluorescent methotrexate labeling and flow cytometric analysis of cells containing low levels of dihydrofolate reductase. J Biol Chem 1986; 261:6285 - 92; PMID: 3700393
   PubMed Web of Science ®Google Scholar