The NBDs that wouldn't die

References

• Dietrich D, Schmuths H, De Marcos Lousa C, Baldwin JM, Baldwin SA, Baker A, et al. Mutations in the Arabidopsis peroxisomal ABC transporter COMATOSE allow differentiation between multiple functions in planta: Insights from an allelic series. Mol Biol Cell 2009; 20:530 - 543
   PubMed Web of Science ®Google Scholar
• Frelet A, Klein M. Insight in eukaryotic ABC transporter function by mutation analysis. FEBS Lett 2006; 580:1064 - 1084
   PubMed Web of Science ®Google Scholar
• Evans GL, Ni B, Hrycyna CA, Chen D, Ambudkar SV, Pastan I, et al. Heterologous expression systems for P-glycoprotein: E. coli, yeast and baculovirus. J Bioenerg Biomembr 1995; 27:43 - 52
   PubMed Web of Science ®Google Scholar
• Morita M, Kurisu M, Kashiwayama Y, Yokota S, Imanaka T. ATP-binding and -hydrolysis activities of ALDP (ABCD1) and ALDRP (ABCD2), human peroxisomal ABC proteins, overexpressed in Sf21 cells. Biol Pharm Bull 2006; 29:1836 - 1842
   PubMed Web of Science ®Google Scholar
• Berridge G, Walker JA, Callaghan R, Kerr ID. The nucleotide-binding domains of P-glycoprotein: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling. Eur J Biochem 2003; 270:1483 - 1492
   PubMedGoogle Scholar
• Wang C, Castro AF, Wilkes DM, Altenberg GA. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein. Biochem J 1999; 338:77 - 81
   PubMed Web of Science ®Google Scholar
• Stratford FL, Ramjeesingh M, Cheung JC, Huan LJ, Bear CE. The Walker B motif of the second nucleotide-binding domain (NBD2) of CFTR plays a key role in ATPase activity by the NBD1–NBD2 heterodimer. Biochem J 2007; 401:581 - 586
   PubMed Web of Science ®Google Scholar
• Cheung JC, Kim Chiaw P, Pasyk S, Bear CE. Molecular basis for the ATPase activity of CFTR. Arch Biochem Biophys 2008; 476:95 - 100
   PubMed Web of Science ®Google Scholar
• Zaitseva J, Jenewein S, Jumpertz T, Holland IB, Schmitt L. H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB. EMBO J 2005; 24:1901 - 1910
   PubMed Web of Science ®Google Scholar
• Procko E, Ferrin-O'Connell I, Ng SL, Gaudet R. Distinct structural and functional properties of the ATPase sites in an asymmetric ABC transporter. Mol Cell 2006; 24:51 - 62
   PubMed Web of Science ®Google Scholar
• Ernst R, Koch J, Horn C, Tampé R, Schmitt L. Engineering ATPase activity in the isolated ABC cassette of human TAP1. J Biol Chem 2006; 281:27471 - 27480
   PubMed Web of Science ®Google Scholar
• de Wet H, Mikhailov MV, Fotinou C, Dreger M, Craig TJ, Vénien-Bryan C, et al. Studies of the ATPase activity of the ABC protein SUR1. FEBS J 2007; 274:3532 - 3544
   PubMed Web of Science ®Google Scholar
• Zingman LV, Hodgson DM, Bienengraeber M, Karger AB, Kathmann EC, Alekseev AE, et al. Tandem function of nucleotide binding domains confers competence to sulfonylurea receptor in gating ATP-sensitive K+ channels. J Biol Chem 2002; 277:14206 - 14210
   PubMed Web of Science ®Google Scholar
• Ko YH, Thomas PJ, Delannoy MR, Pedersen PL. The cystic fibrosis transmembrane conductance regulator. Overexpression, purification and characterization of wild type and delta F508 mutant forms of the first nucleotide binding fold in fusion with the maltose-binding protein. J Biol Chem 1993; 268:24330 - 24338
   PubMed Web of Science ®Google Scholar
• Smith PC, Karpowich N, Millen L, Moody JE, Rosen J, Thomas PJ, et al. ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer. Mol Cell 2002; 10:139 - 149
   PubMed Web of Science ®Google Scholar
• de Wet H, Proks P, Lafond M, Aittoniemi J, Sansom MS, Flanagan SE, et al. A mutation (R826W) in nucleotide-binding domain 1 of ABCC8 reduces ATPase activity and causes transient neonatal diabetes. EMBO Rep 2008; 9:648 - 654
   Google Scholar
• Procko E, Gaudet R. Functionally important interactions between the nucleotide-binding domains of an antigenic peptide transporter. Biochemistry 2008; 47:5699 - 5708
   PubMed Web of Science ®Google Scholar
• Baginski ES, Foà PP, Zak B. Microdetermination of inorganic phosphate, phospholipids and total phosphate in biologic materials. Clin Chem 1967; 13:326 - 332
   PubMed Web of Science ®Google Scholar
• Chifflet S, Torriglia A, Chiesa R, Tolosa S. A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: application to lens ATPases. Anal Biochem 1988; 168:1 - 4
   PubMed Web of Science ®Google Scholar