1,949
Views
48
CrossRef citations to date
0
Altmetric
Brief Report

High level expression of functional human IgMs in human PER.C6® cells

, , , , , , , , , , , , , , & show all
Pages 163-171 | Received 30 Nov 2008, Accepted 24 Jan 2009, Published online: 01 Mar 2009

References

  • Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256:495 - 497
  • Reichert JM, Valge-Archer VE. Development trends for monoclonal antibody cancer therapeutics. Nature Reviews 2007; 6:349 - 356
  • Collins C, Tsui FWL, Shulman MJ. Differential activation of human and guinea pig complement by pentameric and hexameric IgM. Eur J Immunol 2002; 32:1802 - 1810
  • Wiersma EJ, Collins C, Fazel S, Shulman MJ. Structural and functional analysis of J chain-deficient IgM. J Immunol 1998; 160:5979 - 5989
  • Irie RF, Ollila DW, O'Day S, Morton DL. Phase I pilot clinical trial of human IgM monoclonal antibody to ganglioside GM3 in patients with metastatic melanoma. Cancer Immunol Immunother 2004; 53:110 - 117
  • Jones PC, Sze LL, Liu PY, Morton DL, Irie RF. Prolonged survival for melanoma patients with elevated IgM antibody to oncofetal antigen. J Natl Cancer Inst 1981; 66:249 - 254
  • Ollert MW, David K, Schmitt C, Hauenschild A, Bredehorst R, Erttmann R, et al. Normal human serum contains a natural IgM antibody cytotoxic for human neuroblastoma cells. Proc Natl Acad Sci USA 1996; 93:4498 - 4503
  • Vollmers HP, O'Connor R, Muller J, Kirchner T, Muller-Hermelink HK. SC-1, a functional human monoclonal antibody against autologous stomach carcinoma cells. Cancer Res 1989; 49:2471 - 2476
  • Brandlein S, Pohle T, Ruoff N, Wozniak E, Muller-Hermelink HK, Vollmers HP. Natural IgM antibodies and immunosurveillance mechanisms against epithelial cancer cells in humans. Cancer Res 2003; 63:7995 - 8005
  • Vollmers HP, Brandlein S. Natural antibodies and cancer. J Autoimmune. 2007; 29:295 - 302
  • Wurm F. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 2004; 22:1393 - 1398
  • Cattaneo A, Neuberger MS. Polymeric immunoglobulin M is secreted by transfectants of non lymphoid cells in the absence of immunoglobulin J chain. EMBO J 1987; 6:2753 - 2758
  • Cacciuttolo MA, Patchan M, Lamey K, Allikmets E, Tsao E. Large-scale production of a monoclonal IgM in a hybridoma suspension culture. BioPharm 1998; 20 - 29
  • Wood CR, Dorner AJ, Morris GE, Alderman EM, Wilson D, O'Hara RM Jr, Kaufman RJ. High level synthesis of immunoglobulins in Chinese hamster ovary cells. J Immunol 1990; 145:3011 - 3016
  • Barnes LM, Bentley CM, Dickson AJ. Stability of protein production from recombinant mammalian cells. Biotechnol Bioeng 2003; 81:631 - 639
  • Faller G, Vollmers HP, Weiglein I, Marx A, Zink C, Pfaff M, Muller-Hermelink HK. HAB-1, a new heteromyeloma for continuous production of human monoclonal antibodies. Br J Cancer 1990; 62:595 - 598
  • Brandlein S, Vollmers HP. Natural IgM antibodies, the ignored weapons in tumor immunity. Histol Histopathol 2004; 897 - 905
  • Vollmers HP, Brandlein S. Death by stress: natural IgM-induced apoptosis. Methods Find Exp Clin Pharmacol 2005; 27:185 - 191
  • Brandlein S, Lorenz J, Ruoff N, Hensel F, Beyer I, Muller J, et al. Human monoclonal IgM antibodies with apoptotic activity isolated from cancer patients. Hum Antibodies 2002; 11:107 - 119
  • Jones D, Kroos N, Anema R, van Montfort B, Vooys A, van der Kraats S, et al. High-level expression of recombinant IgG in the human cell line PER.C6®. Biotechnol Prog 2003; 19:163 - 168
  • 2008; DSM and Crucell press release, “DSM and Crucell announce another key achievement for PER.C6® technology: scaleup of high titer fed-batch process to 250 liters.”
  • Brandlein S, Rauschert N, Rasche L, Dreykluft A, Hensel F, Conzelmann E, et al. The human IgM antibody SAM-6 induces tumor-specific apoptosis with oxidized low-density lipoprotein. Mol Cancer Ther 2007; 6:326 - 333
  • Randall TD, Brewer JW, Corley RB. Direct evidence that J chain regulates the polymeric structure of IgM in antibody-secreting B cells. J Bio Chem 1992; 267:18002 - 18007
  • Tagliavacca L, Anelli T, Fagioli C, Mezghrani A, Ruffato E, Sitia R. The making of a professional secretory cell: architectural and functional changes in the ER during B lymphocyte plasma cell differentiation. Biol Chem 2003; 384:1273 - 1277
  • Kimura N, Toyonaga B, Yoshikai Y, Du R, Mak TW. Sequences and repertoire of the human T cell receptor α and β chain variable region genes in thymocytes. Eur J Immunol 1987; 17:375 - 383
  • Gagnon P, Hensel F, Richieri R. Purification of IgM Monoclonal Antibodies: Manufacturing challenges surround the use of IgM monoclonal. BioPharm Int Sup 2008; 26 - 36