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Fluorescent Citrine-IgG fusion proteins produced in mammalian cells

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Pages 648-661 | Received 09 Aug 2010, Accepted 27 Jul 2010, Published online: 01 Nov 2010

References

  • Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science 1994; 263:802 - 805
  • White J, Stelzer E. Photobleaching GFP reveals protein dynamics inside live cells. Trends Cell Biol 1999; 9:61 - 65
  • Patterson GH, Knobel SM, Sharif WD, Kain SR, Piston DW. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J 1997; 73:2782 - 2790
  • Niswender KD, Blackman SM, Rohde L, Magnuson MA, Piston DW. Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J Microsc 1995; 180:109 - 116
  • Song L, Hennink EJ, Young IT, Tanke HJ. Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys J 1995; 68:2588 - 2600
  • Tsien RY. The green fluorescent protein. Annu Rev Biochem 1998; 67:509 - 544
  • Casey JL, Coley AM, Tilley LM, Foley M. Green fluorescent antibodies: novel in vitro tools. Protein Eng 2000; 13:445 - 452
  • Griep RA, van Twisk C, van der Wolf JM, Schots A. Fluobodies: green fluorescent single-chain Fv fusion proteins. J Immunol Methods 1999; 230:121 - 130
  • Morino K, Katsumi H, Akahori Y, Iba Y, Shinohara M, Ukai Y, et al. Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression. J Immunol Methods 2001; 257:175 - 184
  • Olichon A, Surrey T. Selection of genetically encoded fluorescent single domain antibodies engineered for efficient expression in Escherichia coli. J Biol Chem 2007; 282:36314 - 36320
  • Veettil S, Budisa N, Jung G. Photostability of green and yellow fluorescent proteins with fluorinated chromophores, investigated by fluorescence correlation spectroscopy. Biophys Chem 2008; 136:38 - 43
  • Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol 1999; 58:19 - 30
  • Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science 1996; 273:1392 - 1395
  • Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J Biol Chem 2001; 276:29188 - 29194
  • Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). P Natl Acad Sci USA 2000; 97:11996 - 12001
  • Sbalzarini IF, Mezzacasa A, Helenius A, Koumoutsakos P. Effects of organelle shape on fluorescence recovery after photobleaching. Biophys J 2005; 89:1482 - 1492
  • Li E, Placone J, Merzlyakov M, Hristova K. Quantitative measurements of protein interactions in a crowded cellular environment. Anal Chem 2008; 80:5976 - 5985
  • Dundr M, McNally JG, Cohen J, Misteli T. Quantitation of GFP-fusion proteins in single living cells. J Struct Biol 2002; 140:92 - 99
  • Hirschberg K, Miller CM, Ellenberg J, Presley JF, Siggia ED, Phair RD, et al. Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells. J Cell Biol 1998; 143:1485 - 1503
  • Holtke HJ, Seibl R, Burg J, Muhlegger K, Kessler C. Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system. Biol Chem Hoppe Seyler 1990; 371:929 - 938
  • Kessler C, Holtke HJ, Seibl R, Burg J, Muhlegger K. Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: anti-digoxigenin ELISA principle (digoxigenin system). Biol Chem Hoppe Seyler 1990; 371:917 - 927
  • Ridgway JB, Presta LG, Carter P. ‘Knobs-into-holes’ engineering of antibody CH3 domains for heavy chain heterodimerization. Protein Eng 1996; 9:617 - 621
  • Merchant AM, Zhu Z, Yuan JQ, Goddard A, Adams CW, Presta LG, et al. An efficient route to human bispecific IgG. Nat Biotechnol 1998; 16:677 - 681
  • Nyfeler B, Michnick SW, Hauri HP. Capturing protein interactions in the secretory pathway of living cells. P Natl Acad Sci USA 2005; 102:6350 - 6355
  • Nyfeler B, Hauri HP. Visualization of protein interactions inside the secretory pathway. Biochem Soc Trans 2007; 35:970 - 973
  • Riedemann J, Macaulay VM. IGF1R signalling and its inhibition. Endocr Relat Cancer 2006; 13:33 - 43
  • Chitnis MM, Yuen JS, Protheroe AS, Pollak M, Macaulay VM. The type 1 insulin-like growth factor receptor pathway. Clin Cancer Res 2008; 14:6364 - 6370
  • Beuger V, Kunkele KP, Koll H, Gartner A, Bahner M, Burtscher H, et al. Short-hairpin-RNA-mediated silencing of fucosyltransferase 8 in Chinese-hamster ovary cells for the production of antibodies with enhanced antibody immune effector function. Biotechnol Appl Biochem 2009; 53:31 - 37
  • Brinkmann U, Pai LH, FitzGerald DJ, Willingham M, Pastan I. B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice. P Natl Acad Sci USA 1991; 88:8616 - 8620
  • Pastan I, Lovelace ET, Gallo MG, Rutherford AV, Magnani JL, Willingham MC. Characterization of monoclonal antibodies B1 and B3 that react with mucinous adenocarcinomas. Cancer Res 1991; 51:3781 - 3787
  • Brinkmann U, Reiter Y, Jung SH, Lee B, Pastan I. A recombinant immunotoxin containing a disulfide-stabilized Fv fragment. P Natl Acad Sci USA 1993; 90:7538 - 7542
  • Reiter Y, Brinkmann U, Jung SH, Pastan I, Lee B. Disulfide stabilization of antibody Fv: computer predictions and experimental evaluation. Protein Eng 1995; 8:1323 - 1331
  • Burtrum D, Zhu Z, Lu D, Anderson DM, Prewett M, Pereira DS, et al. A fully human monoclonal antibody to the insulin-like growth factor I receptor blocks ligand-dependent signaling and inhibits human tumor growth in vivo. Cancer Res 2003; 63:8912 - 8921
  • Gong Y, Yao E, Shen R, Goel A, Arcila M, Teruya-Feldstein J, et al. High expression levels of total IGF-1R and sensitivity of NSCLC cells in vitro to an anti-IGF-1R antibody (R1507). PLoS One 2009; 4:7273
  • Maxfield FR, McGraw TE. Endocytic recycling. Nat Rev Mol Cell Biol 2004; 5:121 - 132
  • Ohkuma S, Poole B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. P Natl Acad Sci USA 1978; 75:3327 - 3331
  • Michnick SW, Remy I, Campbell-Valois FX, Vallee-Belisle A, Pelletier JN. Detection of protein-protein interactions by protein fragment complementation strategies. Methods Enzymol 2000; 328:208 - 230