The dynamic pathway of nuclear RNA in eukaryotes

References

• Politz JC, Taneja KL, Singer RH. Characterization of hybridization between synthetic oligodeoxynucleotides and RNA in living cells. Nucleic Acids Res 1995; 23:4946 - 53; http://dx.doi.org/10.1093/nar/23.24.4946; PMID: 8559650
   PubMed Web of Science ®Google Scholar
• Politz JC, Browne ES, Wolf DE, Pederson T. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc Natl Acad Sci U S A 1998; 95:6043 - 8; http://dx.doi.org/10.1073/pnas.95.11.6043; PMID: 9600914
   PubMed Web of Science ®Google Scholar
• Calapez A, Pereira HM, Calado A, Braga J, Rino J, Carvalho C, et al. The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive. J Cell Biol 2002; 159:795 - 805; http://dx.doi.org/10.1083/jcb.200203046; PMID: 12473688
   PubMed Web of Science ®Google Scholar
• Molenaar C, Marras SA, Slats JC, Truffert JC, Lemaître M, Raap AK, et al. Linear 2′ O-Methyl RNA probes for the visualization of RNA in living cells. Nucleic Acids Res 2001; 29:E89 - 9; http://dx.doi.org/10.1093/nar/29.17.e89; PMID: 11522845
   PubMedGoogle Scholar
• Molenaar C, Abdulle A, Gena A, Tanke HJ, Dirks RW. Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells. J Cell Biol 2004; 165:191 - 202; http://dx.doi.org/10.1083/jcb.200310139; PMID: 15117966
   PubMed Web of Science ®Google Scholar
• Tsuji A, Koshimoto H, Sato Y, Hirano M, Sei-Iida Y, Kondo S, et al. Direct observation of specific messenger RNA in a single living cell under a fluorescence microscope. Biophys J 2000; 78:3260 - 74; http://dx.doi.org/10.1016/S0006-3495(00)76862-7; PMID: 10828002
   PubMed Web of Science ®Google Scholar
• Tsuji A, Sato Y, Hirano M, Suga T, Koshimoto H, Taguchi T, et al. Development of a time-resolved fluorometric method for observing hybridization in living cells using fluorescence resonance energy transfer. Biophys J 2001; 81:501 - 15; http://dx.doi.org/10.1016/S0006-3495(01)75717-7; PMID: 11423432
   PubMed Web of Science ®Google Scholar
• Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2′-O-methyl RNA probes in living cells. Nucleic Acids Res 2011; 39:e20; http://dx.doi.org/10.1093/nar/gkq1196; PMID: 21106497
   PubMedGoogle Scholar
• Tadakuma H, Ishihama Y, Shibuya T, Tani T, Funatsu T. Imaging of single mRNA molecules moving within a living cell nucleus. Biochem Biophys Res Commun 2006; 344:772 - 9; http://dx.doi.org/10.1016/j.bbrc.2006.03.202; PMID: 16631111
   PubMed Web of Science ®Google Scholar
• Ozawa T, Natori Y, Sato M, Umezawa Y. Imaging dynamics of endogenous mitochondrial RNA in single living cells. Nat Methods 2007; 4:413 - 9; PMID: 17401370
   PubMed Web of Science ®Google Scholar
• Paige JS, Wu KY, Jaffrey SR. RNA mimics of green fluorescent protein. Science 2011; 333:642 - 6; http://dx.doi.org/10.1126/science.1207339; PMID: 21798953
   PubMed Web of Science ®Google Scholar
• Santangelo PJ, Alonas E, Jung J, Lifland AW, Zurla C. Probes for intracellular RNA imaging in live cells. Methods Enzymol 2012; 505:383 - 99; http://dx.doi.org/10.1016/B978-0-12-388448-0.00028-0; PMID: 22289464
   PubMed Web of Science ®Google Scholar
• Bann DV, Parent LJ. Application of live-cell RNA imaging techniques to the study of retroviral RNA trafficking. Viruses 2012; 4:963 - 79; http://dx.doi.org/10.3390/v4060963; PMID: 22816035
   PubMed Web of Science ®Google Scholar
• Armitage BA. Imaging of RNA in live cells. Curr Opin Chem Biol 2011; 15:806 - 12; http://dx.doi.org/10.1016/j.cbpa.2011.10.006; PMID: 22055496
   PubMed Web of Science ®Google Scholar
• Urbinati CR, Long RM. Techniques for following the movement of single RNAs in living cells. Wiley Interdiscip Rev RNA 2011; 2:601 - 9; http://dx.doi.org/10.1002/wrna.83; PMID: 21957047
   PubMedGoogle Scholar
• Christensen NM, Oparka KJ, Tilsner J. Advances in imaging RNA in plants. Trends Plant Sci 2010; 15:196 - 203; http://dx.doi.org/10.1016/j.tplants.2010.01.005; PMID: 20153241
   PubMed Web of Science ®Google Scholar
• Tyagi S. Imaging intracellular RNA distribution and dynamics in living cells. Nat Methods 2009; 6:331 - 8; http://dx.doi.org/10.1038/nmeth.1321; PMID: 19404252
   PubMed Web of Science ®Google Scholar
• Dictenberg J. Genetic encoding of fluorescent RNA ensures a bright future for visualizing nucleic acid dynamics. Trends Biotechnol 2012; 30:621 - 6; http://dx.doi.org/10.1016/j.tibtech.2012.09.004; PMID: 23127753
   PubMed Web of Science ®Google Scholar
• Politz JC, Tuft RA, Pederson T, Singer RH. Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells. Curr Biol 1999; 9:285 - 91; http://dx.doi.org/10.1016/S0960-9822(99)80136-5; PMID: 10209094
   PubMed Web of Science ®Google Scholar
• Bratu DP, Cha BJ, Mhlanga MM, Kramer FR, Tyagi S. Visualizing the distribution and transport of mRNAs in living cells. Proc Natl Acad Sci U S A 2003; 100:13308 - 13; http://dx.doi.org/10.1073/pnas.2233244100; PMID: 14583593
   PubMed Web of Science ®Google Scholar
• Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Sets of RNA repeated tags and hybridization-sensitive fluorescent probes for distinct images of RNA in a living cell. PLoS One 2010; 5:e13003; http://dx.doi.org/10.1371/journal.pone.0013003; PMID: 20885944
   PubMedGoogle Scholar
• Santangelo PJ, Lifland AW, Curt P, Sasaki Y, Bassell GJ, Lindquist ME, et al. Single molecule-sensitive probes for imaging RNA in live cells. Nat Methods 2009; 6:347 - 9; http://dx.doi.org/10.1038/nmeth.1316; PMID: 19349979
   PubMed Web of Science ®Google Scholar
• Vargas DY, Raj A, Marras SA, Kramer FR, Tyagi S. Mechanism of mRNA transport in the nucleus. Proc Natl Acad Sci U S A 2005; 102:17008 - 13; http://dx.doi.org/10.1073/pnas.0505580102; PMID: 16284251
   PubMed Web of Science ®Google Scholar
• Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, et al. From silencing to gene expression: real-time analysis in single cells. Cell 2004; 116:683 - 98; http://dx.doi.org/10.1016/S0092-8674(04)00171-0; PMID: 15006351
   PubMed Web of Science ®Google Scholar
• Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, et al. In vivo dynamics of RNA polymerase II transcription. Nat Struct Mol Biol 2007; 14:796 - 806; http://dx.doi.org/10.1038/nsmb1280; PMID: 17676063
   PubMed Web of Science ®Google Scholar
• Ben-Ari Y, Brody Y, Kinor N, Mor A, Tsukamoto T, Spector DL, et al. The life of an mRNA in space and time. J Cell Sci 2010; 123:1761 - 74; http://dx.doi.org/10.1242/jcs.062638; PMID: 20427315
   PubMed Web of Science ®Google Scholar
• Brody Y, Neufeld N, Bieberstein N, Causse SZ, Böhnlein EM, Neugebauer KM, et al. The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing. PLoS Biol 2011; 9:e1000573; http://dx.doi.org/10.1371/journal.pbio.1000573; PMID: 21264352
   PubMed Web of Science ®Google Scholar
• Boireau S, Maiuri P, Basyuk E, de la Mata M, Knezevich A, Pradet-Balade B, et al. The transcriptional cycle of HIV-1 in real-time and live cells. J Cell Biol 2007; 179:291 - 304; http://dx.doi.org/10.1083/jcb.200706018; PMID: 17954611
   PubMed Web of Science ®Google Scholar
• Shanbhag NM, Rafalska-Metcalf IU, Balane-Bolivar C, Janicki SM, Greenberg RA. ATM-dependent chromatin changes silence transcription in cis to DNA double-strand breaks. Cell 2010; 141:970 - 81; http://dx.doi.org/10.1016/j.cell.2010.04.038; PMID: 20550933
   PubMed Web of Science ®Google Scholar
• Martins SB, Rino J, Carvalho T, Carvalho C, Yoshida M, Klose JM, et al. Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3′ end of human genes. Nat Struct Mol Biol 2011; 18:1115 - 23; http://dx.doi.org/10.1038/nsmb.2124; PMID: 21892168
   PubMed Web of Science ®Google Scholar
• de Turris V, Nicholson P, Orozco RZ, Singer RH, Mühlemann O. Cotranscriptional effect of a premature termination codon revealed by live-cell imaging. RNA 2011; 17:2094 - 107; http://dx.doi.org/10.1261/rna.02918111; PMID: 22028363
   PubMed Web of Science ®Google Scholar
• Golding I, Cox EC. RNA dynamics in live Escherichia coli cells. Proc Natl Acad Sci U S A 2004; 101:11310 - 5; http://dx.doi.org/10.1073/pnas.0404443101; PMID: 15277674
   PubMed Web of Science ®Google Scholar
• Golding I, Paulsson J, Zawilski SM, Cox EC. Real-time kinetics of gene activity in individual bacteria. Cell 2005; 123:1025 - 36; http://dx.doi.org/10.1016/j.cell.2005.09.031; PMID: 16360033
   PubMed Web of Science ®Google Scholar
• Haim-Vilmovsky L, Gadir N, Herbst RH, Gerst JE. A genomic integration method for the simultaneous visualization of endogenous mRNAs and their translation products in living yeast. RNA 2011; 17:2249 - 55; http://dx.doi.org/10.1261/rna.029637.111; PMID: 22025736
   PubMed Web of Science ®Google Scholar
• Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Localization of ASH1 mRNA particles in living yeast. Mol Cell 1998; 2:437 - 45; http://dx.doi.org/10.1016/S1097-2765(00)80143-4; PMID: 9809065
   PubMed Web of Science ®Google Scholar
• Chubb JR, Trcek T, Shenoy SM, Singer RH. Transcriptional pulsing of a developmental gene. Curr Biol 2006; 16:1018 - 25; http://dx.doi.org/10.1016/j.cub.2006.03.092; PMID: 16713960
   PubMed Web of Science ®Google Scholar
• Muramoto T, Cannon D, Gierlinski M, Corrigan A, Barton GJ, Chubb JR. Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation. Proc Natl Acad Sci U S A 2012; 109:7350 - 5; http://dx.doi.org/10.1073/pnas.1117603109; PMID: 22529358
   PubMed Web of Science ®Google Scholar
• Forrest KM, Gavis ER. Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr Biol 2003; 13:1159 - 68; http://dx.doi.org/10.1016/S0960-9822(03)00451-2; PMID: 12867026
   PubMed Web of Science ®Google Scholar
• Zimyanin VL, Belaya K, Pecreaux J, Gilchrist MJ, Clark A, Davis I, et al. In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization. Cell 2008; 134:843 - 53; http://dx.doi.org/10.1016/j.cell.2008.06.053; PMID: 18775316
   PubMed Web of Science ®Google Scholar
• Yunger S, Rosenfeld L, Garini Y, Shav-Tal Y. Single-allele analysis of transcription kinetics in living mammalian cells. Nat Methods 2010; 7:631 - 3; http://dx.doi.org/10.1038/nmeth.1482; PMID: 20639867
   PubMed Web of Science ®Google Scholar
• Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, et al. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods 2011; 8:165 - 70; http://dx.doi.org/10.1038/nmeth.1551; PMID: 21240280
   PubMed Web of Science ®Google Scholar
• Singh OP, Björkroth B, Masich S, Wieslander L, Daneholt B. The intranuclear movement of Balbiani ring premessenger ribonucleoprotein particles. Exp Cell Res 1999; 251:135 - 46; http://dx.doi.org/10.1006/excr.1999.4490; PMID: 10438579
   PubMed Web of Science ®Google Scholar
• Snaar SP, Verdijk P, Tanke HJ, Dirks RW. Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts. J Cell Sci 2002; 115:321 - 8; PMID: 11839784
   PubMed Web of Science ®Google Scholar
• Braga J, McNally JG, Carmo-Fonseca M. A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching. Biophys J 2007; 92:2694 - 703; http://dx.doi.org/10.1529/biophysj.106.096693; PMID: 17259280
   PubMed Web of Science ®Google Scholar
• Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, et al. Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Curr Biol 2003; 13:161 - 7; http://dx.doi.org/10.1016/S0960-9822(02)01436-7; PMID: 12546792
   PubMed Web of Science ®Google Scholar
• Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL, et al. Dynamics of single mRNPs in nuclei of living cells. Science 2004; 304:1797 - 800; http://dx.doi.org/10.1126/science.1099754; PMID: 15205532
   PubMed Web of Science ®Google Scholar
• Saxton MJ, Jacobson K. Single-particle tracking: applications to membrane dynamics. Annu Rev Biophys Biomol Struct 1997; 26:373 - 99; http://dx.doi.org/10.1146/annurev.biophys.26.1.373; PMID: 9241424
   PubMedGoogle Scholar
• Mor A, Suliman S, Ben-Yishay R, Yunger S, Brody Y, Shav-Tal Y. Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells. Nat Cell Biol 2010; 12:543 - 52; http://dx.doi.org/10.1038/ncb2056; PMID: 20453848
   PubMed Web of Science ®Google Scholar
• Shav-Tal Y, Gruenbaum Y. Single-molecule dynamics of nuclear mRNA. F1000 Biol Rep 2009; 1:29 - 32; PMID: 20948657
   PubMedGoogle Scholar
• Siebrasse JP, Veith R, Dobay A, Leonhardt H, Daneholt B, Kubitscheck U. Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin. Proc Natl Acad Sci U S A 2008; 105:20291 - 6; http://dx.doi.org/10.1073/pnas.0810692105; PMID: 19074261
   PubMed Web of Science ®Google Scholar
• Ishihama Y, Funatsu T. Single molecule tracking of quantum dot-labeled mRNAs in a cell nucleus. Biochem Biophys Res Commun 2009; 381:33 - 8; http://dx.doi.org/10.1016/j.bbrc.2009.02.001; PMID: 19351590
   PubMed Web of Science ®Google Scholar
• Thompson MA, Casolari JM, Badieirostami M, Brown PO, Moerner WE. Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function. Proc Natl Acad Sci U S A 2010; 107:17864 - 71; http://dx.doi.org/10.1073/pnas.1012868107; PMID: 20921361
   PubMed Web of Science ®Google Scholar
• Grünwald D, Martin RM, Buschmann V, Bazett-Jones DP, Leonhardt H, Kubitscheck U, et al. Probing intranuclear environments at the single-molecule level. Biophys J 2008; 94:2847 - 58; http://dx.doi.org/10.1529/biophysj.107.115014; PMID: 18065482
   PubMed Web of Science ®Google Scholar
• Bancaud A, Huet S, Daigle N, Mozziconacci J, Beaudouin J, Ellenberg J. Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. EMBO J 2009; 28:3785 - 98; http://dx.doi.org/10.1038/emboj.2009.340; PMID: 19927119
   PubMed Web of Science ®Google Scholar
• Matsumoto K, Tanaka KJ, Aoki K, Sameshima M, Tsujimoto M. Visualization of the reconstituted FRGY2-mRNA complexes by electron microscopy. Biochem Biophys Res Commun 2003; 306:53 - 8; http://dx.doi.org/10.1016/S0006-291X(03)00909-4; PMID: 12788065
   PubMed Web of Science ®Google Scholar
• Batisse J, Batisse C, Budd A, Böttcher B, Hurt E. Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem 2009; 284:34911 - 7; http://dx.doi.org/10.1074/jbc.M109.062034; PMID: 19840948
   PubMed Web of Science ®Google Scholar
• Skabkin MA, Kiselyova OI, Chernov KG, Sorokin AV, Dubrovin EV, Yaminsky IV, et al. Structural organization of mRNA complexes with major core mRNP protein YB-1. Nucleic Acids Res 2004; 32:5621 - 35; http://dx.doi.org/10.1093/nar/gkh889; PMID: 15494450
   PubMed Web of Science ®Google Scholar
• Braga J, Rino J, Carmo-Fonseca M. Photobleaching microscopy reveals the dynamics of mRNA-binding proteins inside live cell nuclei. Prog Mol Subcell Biol 2004; 35:119 - 34; http://dx.doi.org/10.1007/978-3-540-74266-1_6; PMID: 15113082
   PubMedGoogle Scholar
• Roussel MR, Tang T. Simulation of mRNA diffusion in the nuclear environment. IET Syst Biol 2012; 6:125 - 33; http://dx.doi.org/10.1049/iet-syb.2011.0032; PMID: 23039693
   PubMed Web of Science ®Google Scholar
• Boulon S, Basyuk E, Blanchard JM, Bertrand E, Verheggen C. Intra-nuclear RNA trafficking: insights from live cell imaging. Biochimie 2002; 84:805 - 13; http://dx.doi.org/10.1016/S0300-9084(02)01438-4; PMID: 12457567
   PubMed Web of Science ®Google Scholar
• Kues T, Dickmanns A, Lührmann R, Peters R, Kubitscheck U. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking. Proc Natl Acad Sci U S A 2001; 98:12021 - 6; http://dx.doi.org/10.1073/pnas.211250098; PMID: 11593012
   PubMed Web of Science ®Google Scholar
• Grünwald D, Spottke B, Buschmann V, Kubitscheck U. Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells. Mol Biol Cell 2006; 17:5017 - 27; http://dx.doi.org/10.1091/mbc.E06-06-0559; PMID: 16987963
   PubMed Web of Science ®Google Scholar
• Handwerger KE, Murphy C, Gall JG. Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle. J Cell Biol 2003; 160:495 - 504; http://dx.doi.org/10.1083/jcb.200212024; PMID: 12591912
   PubMed Web of Science ®Google Scholar
• Ali GS, Prasad KV, Hanumappa M, Reddy AS. Analyses of in vivo interaction and mobility of two spliceosomal proteins using FRAP and BiFC. PLoS One 2008; 3:e1953; http://dx.doi.org/10.1371/journal.pone.0001953; PMID: 18414657
   PubMed Web of Science ®Google Scholar
• Rino J, Carvalho T, Braga J, Desterro JM, Lührmann R, Carmo-Fonseca M. A stochastic view of spliceosome assembly and recycling in the nucleus. PLoS Comput Biol 2007; 3:2019 - 31; http://dx.doi.org/10.1371/journal.pcbi.0030201; PMID: 17967051
   PubMed Web of Science ®Google Scholar
• Sapra AK, Ankö ML, Grishina I, Lorenz M, Pabis M, Poser I, et al. SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo. Mol Cell 2009; 34:179 - 90; http://dx.doi.org/10.1016/j.molcel.2009.02.031; PMID: 19394295
   PubMed Web of Science ®Google Scholar
• Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, et al. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol 2010; 191:75 - 86; http://dx.doi.org/10.1083/jcb.201004030; PMID: 20921136
   PubMed Web of Science ®Google Scholar
• Görnemann J, Kotovic KM, Hujer K, Neugebauer KM. Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol Cell 2005; 19:53 - 63; http://dx.doi.org/10.1016/j.molcel.2005.05.007; PMID: 15989964
   PubMed Web of Science ®Google Scholar
• Beyer AL, Osheim YN. Splice site selection, rate of splicing, and alternative splicing on nascent transcripts. Genes Dev 1988; 2:754 - 65; http://dx.doi.org/10.1101/gad.2.6.754; PMID: 3138163
   PubMed Web of Science ®Google Scholar
• Wetterberg I, Baurén G, Wieslander L. The intranuclear site of excision of each intron in Balbiani ring 3 pre-mRNA is influenced by the time remaining to transcription termination and different excision efficiencies for the various introns. RNA 1996; 2:641 - 51; PMID: 8756407
   PubMed Web of Science ®Google Scholar
• Audibert A, Weil D, Dautry F. In vivo kinetics of mRNA splicing and transport in mammalian cells. Mol Cell Biol 2002; 22:6706 - 18; http://dx.doi.org/10.1128/MCB.22.19.6706-6718.2002; PMID: 12215528
   PubMed Web of Science ®Google Scholar
• Singh J, Padgett RA. Rates of in situ transcription and splicing in large human genes. Nat Struct Mol Biol 2009; 16:1128 - 33; http://dx.doi.org/10.1038/nsmb.1666; PMID: 19820712
   PubMed Web of Science ®Google Scholar
• Clelland AK, Bales AB, Sleeman JE. Changes in intranuclear mobility of mature snRNPs provide a mechanism for splicing defects in spinal muscular atrophy. J Cell Sci 2012; 125:2626 - 37; http://dx.doi.org/10.1242/jcs.096867; PMID: 22393244
   PubMed Web of Science ®Google Scholar
• Gallardo F, Laterreur N, Cusanelli E, Ouenzar F, Querido E, Wellinger RJ, et al. Live cell imaging of telomerase RNA dynamics reveals cell cycle-dependent clustering of telomerase at elongating telomeres. Mol Cell 2011; 44:819 - 27; http://dx.doi.org/10.1016/j.molcel.2011.09.020; PMID: 22152484
   PubMed Web of Science ®Google Scholar
• Holt I, Mittal S, Furling D, Butler-Browne GS, Brook JD, Morris GE. Defective mRNA in myotonic dystrophy accumulates at the periphery of nuclear splicing speckles. Genes Cells 2007; 12:1035 - 48; http://dx.doi.org/10.1111/j.1365-2443.2007.01112.x; PMID: 17825047
   PubMed Web of Science ®Google Scholar
• Smith KP, Byron M, Johnson C, Xing Y, Lawrence JB. Defining early steps in mRNA transport: mutant mRNA in myotonic dystrophy type I is blocked at entry into SC-35 domains. J Cell Biol 2007; 178:951 - 64; http://dx.doi.org/10.1083/jcb.200706048; PMID: 17846170
   PubMed Web of Science ®Google Scholar
• Querido E, Gallardo F, Beaudoin M, Ménard C, Chartrand P. Stochastic and reversible aggregation of mRNA with expanded CUG-triplet repeats. J Cell Sci 2011; 124:1703 - 14; http://dx.doi.org/10.1242/jcs.073270; PMID: 21511730
   PubMed Web of Science ®Google Scholar
• Spann P, Feinerman M, Sperling J, Sperling R. Isolation and visualization of large compact ribonucleoprotein particles of specific nuclear RNAs. Proc Natl Acad Sci U S A 1989; 86:466 - 70; http://dx.doi.org/10.1073/pnas.86.2.466; PMID: 2521390
   PubMed Web of Science ®Google Scholar
• Iborra FJ, Jackson DA, Cook PR. The path of transcripts from extra-nucleolar synthetic sites to nuclear pores: transcripts in transit are concentrated in discrete structures containing SR proteins. J Cell Sci 1998; 111:2269 - 82; PMID: 9664048
   PubMed Web of Science ®Google Scholar
• Panté N, Jarmolowski A, Izaurralde E, Sauder U, Baschong W, Mattaj IW. Visualizing nuclear export of different classes of RNA by electron microscopy. RNA 1997; 3:498 - 513; PMID: 9149231
   PubMed Web of Science ®Google Scholar
• Huang S, Deerinck TJ, Ellisman MH, Spector DL. In vivo analysis of the stability and transport of nuclear poly(A)+ RNA. J Cell Biol 1994; 126:877 - 99; http://dx.doi.org/10.1083/jcb.126.4.877; PMID: 7519622
   PubMed Web of Science ®Google Scholar
• Dworetzky SI, Feldherr CM. Translocation of RNA-coated gold particles through the nuclear pores of oocytes. J Cell Biol 1988; 106:575 - 84; http://dx.doi.org/10.1083/jcb.106.3.575; PMID: 2450095
   PubMed Web of Science ®Google Scholar
• Iborra FJ, Jackson DA, Cook PR. The path of RNA through nuclear pores: apparent entry from the sides into specialized pores. J Cell Sci 2000; 113:291 - 302; PMID: 10633080
   PubMed Web of Science ®Google Scholar
• Yang W, Gelles J, Musser SM. Imaging of single-molecule translocation through nuclear pore complexes. Proc Natl Acad Sci U S A 2004; 101:12887 - 92; http://dx.doi.org/10.1073/pnas.0403675101; PMID: 15306682
   PubMed Web of Science ®Google Scholar
• Ribbeck K, Görlich D. Kinetic analysis of translocation through nuclear pore complexes. EMBO J 2001; 20:1320 - 30; http://dx.doi.org/10.1093/emboj/20.6.1320; PMID: 11250898
   PubMed Web of Science ®Google Scholar
• Yamada J, Phillips JL, Patel S, Goldfien G, Calestagne-Morelli A, Huang H, et al. A bimodal distribution of two distinct categories of intrinsically disordered structures with separate functions in FG nucleoporins. Mol Cell Proteomics 2010; 9:2205 - 24; http://dx.doi.org/10.1074/mcp.M000035-MCP201; PMID: 20368288
   PubMed Web of Science ®Google Scholar
• Mehlin H, Daneholt B, Skoglund U. Structural interaction between the nuclear pore complex and a specific translocating RNP particle. J Cell Biol 1995; 129:1205 - 16; http://dx.doi.org/10.1083/jcb.129.5.1205; PMID: 7775568
   PubMed Web of Science ®Google Scholar
• Kiseleva E, Goldberg MW, Allen TD, Akey CW. Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export. J Cell Sci 1998; 111:223 - 36; PMID: 9405308
   PubMed Web of Science ®Google Scholar
• Fiserova J, Richards SA, Wente SR, Goldberg MW. Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex. J Cell Sci 2010; 123:2773 - 80; http://dx.doi.org/10.1242/jcs.070730; PMID: 20647373
   PubMed Web of Science ®Google Scholar
• Mor A, Shav-Tal Y. Dynamics and kinetics of nucleo-cytoplasmic mRNA export. Wiley Interdiscip Rev RNA 2010; 1:388 - 401; http://dx.doi.org/10.1002/wrna.41; PMID: 21956938
   PubMedGoogle Scholar
• Grünwald D, Singer RH, Rout M. Nuclear export dynamics of RNA-protein complexes. Nature 2011; 475:333 - 41; http://dx.doi.org/10.1038/nature10318; PMID: 21776079
   PubMed Web of Science ®Google Scholar
• Stevens BJ, Swift H. RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J Cell Biol 1966; 31:55 - 77; http://dx.doi.org/10.1083/jcb.31.1.55; PMID: 5971975
   PubMed Web of Science ®Google Scholar
• Mehlin H, Daneholt B, Skoglund U. Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography. Cell 1992; 69:605 - 13; http://dx.doi.org/10.1016/0092-8674(92)90224-Z; PMID: 1586943
   PubMed Web of Science ®Google Scholar
• Skoglund U, Andersson K, Björkroth B, Lamb MM, Daneholt B. Visualization of the formation and transport of a specific hnRNP particle. Cell 1983; 34:847 - 55; http://dx.doi.org/10.1016/0092-8674(83)90542-1; PMID: 6556087
   PubMed Web of Science ®Google Scholar
• Kiseleva E, Goldberg MW, Daneholt B, Allen TD. RNP export is mediated by structural reorganization of the nuclear pore basket. J Mol Biol 1996; 260:304 - 11; http://dx.doi.org/10.1006/jmbi.1996.0401; PMID: 8757794
   PubMed Web of Science ®Google Scholar
• Siebrasse JP, Kaminski T, Kubitscheck U. Nuclear export of single native mRNA molecules observed by light sheet fluorescence microscopy. Proc Natl Acad Sci U S A 2012; 109:9426 - 31; http://dx.doi.org/10.1073/pnas.1201781109; PMID: 22615357
   PubMed Web of Science ®Google Scholar
• Noble KN, Tran EJ, Alcázar-Román AR, Hodge CA, Cole CN, Wente SR. The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 2011; 25:1065 - 77; http://dx.doi.org/10.1101/gad.2040611; PMID: 21576266
   PubMed Web of Science ®Google Scholar
• Verschure PJ, van der Kraan I, Manders EM, Hoogstraten D, Houtsmuller AB, van Driel R. Condensed chromatin domains in the mammalian nucleus are accessible to large macromolecules. EMBO Rep 2003; 4:861 - 6; http://dx.doi.org/10.1038/sj.embor.embor922; PMID: 12947417
   PubMed Web of Science ®Google Scholar
• Seksek O, Biwersi J, Verkman AS. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus. J Cell Biol 1997; 138:131 - 42; http://dx.doi.org/10.1083/jcb.138.1.131; PMID: 9214387
   PubMed Web of Science ®Google Scholar
• Cremer T, Cremer C. Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat Rev Genet 2001; 2:292 - 301; http://dx.doi.org/10.1038/35066075; PMID: 11283701
   PubMed Web of Science ®Google Scholar
• Zachar Z, Kramer J, Mims IP, Bingham PM. Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans. J Cell Biol 1993; 121:729 - 42; http://dx.doi.org/10.1083/jcb.121.4.729; PMID: 8491768
   PubMed Web of Science ®Google Scholar
• Fakan S. Perichromatin fibrils are in situ forms of nascent transcripts. Trends Cell Biol 1994; 4:86 - 90; http://dx.doi.org/10.1016/0962-8924(94)90180-5; PMID: 14731598
   PubMedGoogle Scholar
• Spector DL. The dynamics of chromosome organization and gene regulation. Annu Rev Biochem 2003; 72:573 - 608; http://dx.doi.org/10.1146/annurev.biochem.72.121801.161724; PMID: 14527325
   PubMed Web of Science ®Google Scholar
• Niedojadlo J, Perret-Vivancos C, Kalland KH, Cmarko D, Cremer T, van Driel R, et al. Transcribed DNA is preferentially located in the perichromatin region of mammalian cell nuclei. Exp Cell Res 2011; 317:433 - 44; http://dx.doi.org/10.1016/j.yexcr.2010.10.026; PMID: 21056558
   PubMed Web of Science ®Google Scholar
• Cook PR. A model for all genomes: the role of transcription factories. J Mol Biol 2010; 395:1 - 10; http://dx.doi.org/10.1016/j.jmb.2009.10.031; PMID: 19852969
   PubMed Web of Science ®Google Scholar
• Carter KC, Bowman D, Carrington W, Fogarty K, McNeil JA, Fay FS, et al. A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus. Science 1993; 259:1330 - 5; http://dx.doi.org/10.1126/science.8446902; PMID: 8446902
   PubMed Web of Science ®Google Scholar
• Bridger JM, Kalla C, Wodrich H, Weitz S, King JA, Khazaie K, et al. Nuclear RNAs confined to a reticular compartment between chromosome territories. Exp Cell Res 2005; 302:180 - 93; http://dx.doi.org/10.1016/j.yexcr.2004.07.038; PMID: 15561100
   PubMed Web of Science ®Google Scholar
• Reichenzeller M, Burzlaff A, Lichter P, Herrmann H. In vivo observation of a nuclear channel-like system: evidence for a distinct interchromosomal domain compartment in interphase cells. J Struct Biol 2000; 129:175 - 85; http://dx.doi.org/10.1006/jsbi.2000.4224; PMID: 10806067
   PubMed Web of Science ®Google Scholar
• Zirbel RM, Mathieu UR, Kurz A, Cremer T, Lichter P. Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries. Chromosome Res 1993; 1:93 - 106; http://dx.doi.org/10.1007/BF00710032; PMID: 8143096
   PubMed Web of Science ®Google Scholar
• Lawrence JB, Singer RH, Marselle LM. Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. Cell 1989; 57:493 - 502; http://dx.doi.org/10.1016/0092-8674(89)90924-0; PMID: 2541917
   PubMed Web of Science ®Google Scholar
• Xing YG, Lawrence JB. Preservation of specific RNA distribution within the chromatin-depleted nuclear substructure demonstrated by in situ hybridization coupled with biochemical fractionation. J Cell Biol 1991; 112:1055 - 63; http://dx.doi.org/10.1083/jcb.112.6.1055; PMID: 1705562
   PubMed Web of Science ®Google Scholar
• Xing Y, Johnson CV, Dobner PR, Lawrence JB. Higher level organization of individual gene transcription and RNA splicing. Science 1993; 259:1326 - 30; http://dx.doi.org/10.1126/science.8446901; PMID: 8446901
   PubMed Web of Science ®Google Scholar
• Dirks RW, Daniël KC, Raap AK. RNAs radiate from gene to cytoplasm as revealed by fluorescence in situ hybridization. J Cell Sci 1995; 108:2565 - 72; PMID: 7593297
   PubMed Web of Science ®Google Scholar
• Krull S, Dörries J, Boysen B, Reidenbach S, Magnius L, Norder H, et al. Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion. EMBO J 2010; 29:1659 - 73; http://dx.doi.org/10.1038/emboj.2010.54; PMID: 20407419
   PubMed Web of Science ®Google Scholar
• Albiez H, Cremer M, Tiberi C, Vecchio L, Schermelleh L, Dittrich S, et al. Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks. Chromosome Res 2006; 14:707 - 33; http://dx.doi.org/10.1007/s10577-006-1086-x; PMID: 17115328
   PubMed Web of Science ®Google Scholar
• Tutucci E, Stutz F. Keeping mRNPs in check during assembly and nuclear export. Nat Rev Mol Cell Biol 2011; 12:377 - 84; http://dx.doi.org/10.1038/nrm3119; PMID: 21602906
   PubMed Web of Science ®Google Scholar
• Zhou Z, Luo MJ, Straesser K, Katahira J, Hurt E, Reed R. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. Nature 2000; 407:401 - 5; http://dx.doi.org/10.1038/35030160; PMID: 11014198
   PubMed Web of Science ®Google Scholar
• Strässer K, Masuda S, Mason P, Pfannstiel J, Oppizzi M, Rodriguez-Navarro S, et al. TREX is a conserved complex coupling transcription with messenger RNA export. Nature 2002; 417:304 - 8; http://dx.doi.org/10.1038/nature746; PMID: 11979277
   PubMed Web of Science ®Google Scholar
• Stutz F, Bachi A, Doerks T, Braun IC, Séraphin B, Wilm M, et al. REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export. RNA 2000; 6:638 - 50; http://dx.doi.org/10.1017/S1355838200000078; PMID: 10786854
   PubMed Web of Science ®Google Scholar
• Bachi A, Braun IC, Rodrigues JP, Panté N, Ribbeck K, von Kobbe C, et al. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. RNA 2000; 6:136 - 58; http://dx.doi.org/10.1017/S1355838200991994; PMID: 10668806
   PubMed Web of Science ®Google Scholar
• Katahira J, Strässer K, Podtelejnikov A, Mann M, Jung JU, Hurt E. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J 1999; 18:2593 - 609; http://dx.doi.org/10.1093/emboj/18.9.2593; PMID: 10228171
   PubMed Web of Science ®Google Scholar
• Wickramasinghe VO, McMurtrie PI, Mills AD, Takei Y, Penrhyn-Lowe S, Amagase Y, et al. mRNA export from mammalian cell nuclei is dependent on GANP. Curr Biol 2010; 20:25 - 31; http://dx.doi.org/10.1016/j.cub.2009.10.078; PMID: 20005110
   PubMed Web of Science ®Google Scholar
• Le Hir H, Izaurralde E, Maquat LE, Moore MJ. The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. EMBO J 2000; 19:6860 - 9; http://dx.doi.org/10.1093/emboj/19.24.6860; PMID: 11118221
   PubMed Web of Science ®Google Scholar
• Le Hir H, Gatfield D, Izaurralde E, Moore MJ. The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay. EMBO J 2001; 20:4987 - 97; http://dx.doi.org/10.1093/emboj/20.17.4987; PMID: 11532962
   PubMed Web of Science ®Google Scholar
• Björk P, Jin S, Zhao J, Singh OP, Persson JO, Hellman U, et al. Specific combinations of SR proteins associate with single pre-messenger RNAs in vivo and contribute different functions. J Cell Biol 2009; 184:555 - 68; http://dx.doi.org/10.1083/jcb.200806156; PMID: 19221196
   PubMed Web of Science ®Google Scholar
• Schmidt U, Richter K, Berger AB, Lichter P. In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains. J Cell Biol 2006; 172:373 - 81; http://dx.doi.org/10.1083/jcb.200503061; PMID: 16431928
   PubMed Web of Science ®Google Scholar
• Cullen BR. Nuclear RNA export. J Cell Sci 2003; 116:587 - 97; http://dx.doi.org/10.1242/jcs.00268; PMID: 12538759
   PubMed Web of Science ®Google Scholar
• Lang I, Scholz M, Peters R. Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J Cell Biol 1986; 102:1183 - 90; http://dx.doi.org/10.1083/jcb.102.4.1183; PMID: 2420804
   PubMed Web of Science ®Google Scholar
• Braga J, Desterro JM, Carmo-Fonseca M. Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes. Mol Biol Cell 2004; 15:4749 - 60; http://dx.doi.org/10.1091/mbc.E04-06-0496; PMID: 15292455
   PubMed Web of Science ®Google Scholar
• English BP, Hauryliuk V, Sanamrad A, Tankov S, Dekker NH, Elf J. Single-molecule investigations of the stringent response machinery in living bacterial cells. Proc Natl Acad Sci U S A 2011; 108:E365 - 73; http://dx.doi.org/10.1073/pnas.1102255108; PMID: 21730169
   PubMedGoogle Scholar
• Golding I, Cox EC. Physical nature of bacterial cytoplasm. Phys Rev Lett 2006; 96:098102; http://dx.doi.org/10.1103/PhysRevLett.96.098102; PMID: 16606319
   PubMedGoogle Scholar
• Schnell S, Hancock R. The intranuclear environment. Methods Mol Biol 2008; 463:3 - 19; http://dx.doi.org/10.1007/978-1-59745-406-3_1; PMID: 18951157
   PubMedGoogle Scholar
• Richter K, Nessling M, Lichter P. Experimental evidence for the influence of molecular crowding on nuclear architecture. J Cell Sci 2007; 120:1673 - 80; http://dx.doi.org/10.1242/jcs.03440; PMID: 17430977
   PubMed Web of Science ®Google Scholar
• Hameed FM, Rao M, Shivashankar GV. Dynamics of passive and active particles in the cell nucleus. PLoS One 2012; 7:e45843; http://dx.doi.org/10.1371/journal.pone.0045843; PMID: 23077497
   PubMedGoogle Scholar
• Dross N, Spriet C, Zwerger M, Müller G, Waldeck W, Langowski J. Mapping eGFP oligomer mobility in living cell nuclei. PLoS One 2009; 4:e5041; http://dx.doi.org/10.1371/journal.pone.0005041; PMID: 19347038
   PubMed Web of Science ®Google Scholar
• Hinde E, Cardarelli F, Digman MA, Gratton E. In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow. Proc Natl Acad Sci U S A 2010; 107:16560 - 5; http://dx.doi.org/10.1073/pnas.1006731107; PMID: 20823232
   PubMed Web of Science ®Google Scholar
• Speese SD, Ashley J, Jokhi V, Nunnari J, Barria R, Li Y, et al. Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling. Cell 2012; 149:832 - 46; http://dx.doi.org/10.1016/j.cell.2012.03.032; PMID: 22579286
   PubMed Web of Science ®Google Scholar
• Montpetit B, Weis K. Cell biology. An alternative route for nuclear mRNP export by membrane budding. Science 2012; 336:809 - 10; http://dx.doi.org/10.1126/science.1222243; PMID: 22605737
   PubMed Web of Science ®Google Scholar
• Muranyi W, Haas J, Wagner M, Krohne G, Koszinowski UH. Cytomegalovirus recruitment of cellular kinases to dissolve the nuclear lamina. Science 2002; 297:854 - 7; http://dx.doi.org/10.1126/science.1071506; PMID: 12161659
   PubMed Web of Science ®Google Scholar
• Stewart M. Ratcheting mRNA out of the nucleus. Mol Cell 2007; 25:327 - 30; http://dx.doi.org/10.1016/j.molcel.2007.01.016; PMID: 17289581
   PubMed Web of Science ®Google Scholar
• Nachury MV, Weis K. The direction of transport through the nuclear pore can be inverted. Proc Natl Acad Sci U S A 1999; 96:9622 - 7; http://dx.doi.org/10.1073/pnas.96.17.9622; PMID: 10449743
   PubMed Web of Science ®Google Scholar
• Kopito RB, Elbaum M. Reversibility in nucleocytoplasmic transport. Proc Natl Acad Sci U S A 2007; 104:12743 - 8; http://dx.doi.org/10.1073/pnas.0702690104; PMID: 17646647
   PubMed Web of Science ®Google Scholar
• Booth-Gauthier EA, Alcoser TA, Yang G, Dahl KN. Force-induced changes in subnuclear movement and rheology. Biophys J 2012; 103:2423 - 31; http://dx.doi.org/10.1016/j.bpj.2012.10.039; PMID: 23260044
   PubMed Web of Science ®Google Scholar
• Dahl KN, Kalinowski A. Nucleoskeleton mechanics at a glance. J Cell Sci 2011; 124:675 - 8; http://dx.doi.org/10.1242/jcs.069096; PMID: 21321324
   PubMed Web of Science ®Google Scholar
• Chen BP, Li YS, Zhao Y, Chen KD, Li S, Lao J, et al. DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress. Physiol Genomics 2001; 7:55 - 63; http://dx.doi.org/10.1006/geno.2001.6511; PMID: 11595792
   PubMed Web of Science ®Google Scholar
• Poh YC, Shevtsov SP, Chowdhury F, Wu DC, Na S, Dundr M, et al. Dynamic force-induced direct dissociation of protein complexes in a nuclear body in living cells. Nat Commun 2012; 3:866; http://dx.doi.org/10.1038/ncomms1873; PMID: 22643893
   PubMed Web of Science ®Google Scholar
• Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, et al. The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Mol Cell 2010; 39:925 - 38; http://dx.doi.org/10.1016/j.molcel.2010.08.011; PMID: 20797886
   PubMed Web of Science ®Google Scholar
• Wilusz JE, Freier SM, Spector DL. 3′ end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNA. Cell 2008; 135:919 - 32; http://dx.doi.org/10.1016/j.cell.2008.10.012; PMID: 19041754
   PubMed Web of Science ®Google Scholar
• Audas TE, Jacob MD, Lee S. Immobilization of proteins in the nucleolus by ribosomal intergenic spacer noncoding RNA. Mol Cell 2012; 45:147 - 57; http://dx.doi.org/10.1016/j.molcel.2011.12.012; PMID: 22284675
   PubMed Web of Science ®Google Scholar
• Borah S, Darricarrère N, Darnell A, Myoung J, Steitz JA. A viral nuclear noncoding RNA binds re-localized poly(A) binding protein and is required for late KSHV gene expression. PLoS Pathog 2011; 7:e1002300; http://dx.doi.org/10.1371/journal.ppat.1002300; PMID: 22022268
   PubMedGoogle Scholar
• Borah S, Nichols LA, Hassman LM, Kedes DH, Steitz JA. Tracking expression and subcellular localization of RNA and protein species using high-throughput single cell imaging flow cytometry. RNA 2012; 18:1573 - 9; http://dx.doi.org/10.1261/rna.033126.112; PMID: 22745225
   PubMed Web of Science ®Google Scholar
• Lim F, Downey TP, Peabody DS. Translational repression and specific RNA binding by the coat protein of the Pseudomonas phage PP7. J Biol Chem 2001; 276:22507 - 13; http://dx.doi.org/10.1074/jbc.M102411200; PMID: 11306589
   PubMed Web of Science ®Google Scholar
• Schönberger J, Hammes UZ, Dresselhaus T. In vivo visualization of RNA in plants cells using the λN₂₂ system and a GATEWAY-compatible vector series for candidate RNAs. Plant J 2012; 71:173 - 81; http://dx.doi.org/10.1111/j.1365-313X.2012.04923.x; PMID: 22268772
   PubMed Web of Science ®Google Scholar
• Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH. Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods 2013; 10:119 - 21; http://dx.doi.org/10.1038/nmeth.2305; PMID: 23263691
   PubMed Web of Science ®Google Scholar
• Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Science 2011; 332:475 - 8; http://dx.doi.org/10.1126/science.1202142; PMID: 21512033
   PubMed Web of Science ®Google Scholar
• Lange S, Katayama Y, Schmid M, Burkacky O, Bräuchle C, Lamb DC, et al. Simultaneous transport of different localized mRNA species revealed by live-cell imaging. Traffic 2008; 9:1256 - 67; http://dx.doi.org/10.1111/j.1600-0854.2008.00763.x; PMID: 18485054
   PubMed Web of Science ®Google Scholar
• Daigle N, Ellenberg J. LambdaN-GFP: an RNA reporter system for live-cell imaging. Nat Methods 2007; 4:633 - 6; http://dx.doi.org/10.1038/nmeth1065; PMID: 17603490
   PubMed Web of Science ®Google Scholar
• Wu B, Chao JA, Singer RH. Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. Biophys J 2012; 102:2936 - 44; http://dx.doi.org/10.1016/j.bpj.2012.05.017; PMID: 22735544
   PubMed Web of Science ®Google Scholar