4,154
Views
184
CrossRef citations to date
0
Altmetric
Methods & Technologies

Enhanced chemiluminescence (ECL) for routine immunoblotting

An inexpensive alternative to commercially available kits

&
Pages 121-122 | Received 23 May 2011, Accepted 25 May 2011, Published online: 30 Jun 2011

References

  • Burnette WN. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 1981; 112:195 - 203
  • Lie PP, Cheng CY, Mruk DD. The biology of the desmosome-like junction: a versatile anchoring junction and signal transducer in the seminiferous epithelium. Int Rev Cell Mol Biol 2011; 286:223 - 269
  • Su L, Cheng CY, Mruk DD. Drug transporter, P-glycoprotein (MDR1), is an integrated component of the mammalian blood-testis barrier. Int J Biochem Cell Biol 2009; 41:2578 - 2587
  • Xia W, Mruk DD, Lee WM, Cheng CY. Differential interactions between transforming growth factor-β3/TβR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion. J Biol Chem 2006; 281:16799 - 16813
  • Yan HHN, Mruk DD, Lee WM, Cheng CY. Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells. FASEB J 2008; 22:1945 - 1459
  • Haan C, Behrmann I. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background. J Immunol Methods 2007; 318:11 - 19
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680 - 685
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitro-cellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350 - 4354