103
Views
0
CrossRef citations to date
0
Altmetric
Research Notes

An Efficient Method to Prepare PCR Cloning Vectors

, , &
Pages 240-242 | Received 03 Jun 2009, Accepted 06 Aug 2009, Published online: 22 Jun 2018

References

  • Borovkov, A. Y. and Rivkin, M. I. 1997. XcmI-containing vector for direct cloning of PCR products. Biotechniques 22:812-814. Chuang, S. E., Wang, K. C. and Cheng, A. L. 1995. Single-step direct cloning of PDC products. Trends Genet. 11:7-8.
  • Holton, T. A. and Graham, M. W. 1991. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res. 19:1156.
  • Kovalic, D., Kwak, J. H. and Weisblum, B. 1991. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19:4560.
  • Marchuk, D., Drumm, M., Saulino, A. and Collins, F. S. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
  • Mead, D. A., Pey, N. K., Hermstadt, C., Marcil, R. A. and Smith, L. M. 1991. A universal method for the direct cloning of PCR amplified nucleic acid. Biotechnology 9:657-663.
  • Sambrook, J. and Russell, D. W. 2001. Molecular cloning: a laboratory manual. p8.35. Cold Spring Harbor Laboratory Press, New York.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.