Extra Virgin Olive Oil Phenols Suppress Migration and Invasion of T24 Human Bladder Cancer Cells Through Modulation of Matrix Metalloproteinase-2

Andrea CocciaDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, ItalyCorrespondence[email protected]

Daniela BastianelliDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, Italy

Luciana MoscaDepartment of Biochemical Sciences, “Sapienza” University of Rome, Roma, Italy

Roberto MonticoloDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, Italy

Isabella PanuccioDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, Italy

Antonio CarboneDepartment of Medical-Surgical Sciences and Biotechnologies, Urology Unit, “Sapienza” University of Rome, Latina, Italy

Antonella CalogeroDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, Italy

Eugenio LendaroDepartment of Medical-Surgical Sciences and Biotechnologies, “Sapienza” University of Rome, Latina, Italy

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Supplemental material

Supplementary Figure 2. We calculated IC50 (the dose that inhibits cell viability by 50%) through a non linear regression analysis of the dose response curve obtained by MTT assay.

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Supplementary Figure 1. EVOO-E alter MMP-2 mRNA expression also in HT1197 cells. The ratios of the MMP-2 (A), MMP-9 (B), TIMP-1 (C), TIMP-2 (D) mRNA vs 18s mRNA are presented as means ±SEM. *** P<0.001 untreated cells

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Extra Virgin Olive Oil Phenols Suppress Migration and Invasion of T24 Human Bladder Cancer Cells Through Modulation of Matrix Metalloproteinase-2

Supplementary Figure 2. We calculated IC50 (the dose that inhibits cell viability by 50%) through a non linear regression analysis of the dose response curve obtained by MTT assay.

Supplementary Figure 1. EVOO-E alter MMP-2 mRNA expression also in HT1197 cells. The ratios of the MMP-2 (A), MMP-9 (B), TIMP-1 (C), TIMP-2 (D) mRNA vs 18s mRNA are presented as means ±SEM. *** P<0.001 untreated cells

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Extra Virgin Olive Oil Phenols Suppress Migration and Invasion of T24 Human Bladder Cancer Cells Through Modulation of Matrix Metalloproteinase-2

Supplementary Figure 2. We calculated IC50 (the dose that inhibits cell viability by 50%) through a non linear regression analysis of the dose response curve obtained by MTT assay.

Supplementary Figure 1. EVOO-E alter MMP-2 mRNA expression also in HT1197 cells. The ratios of the MMP-2 (A), MMP-9 (B), TIMP-1 (C), TIMP-2 (D) mRNA vs 18s mRNA are presented as means &plusmn;SEM. *** P<0.001 untreated cells

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Supplementary Figure 1. EVOO-E alter MMP-2 mRNA expression also in HT1197 cells. The ratios of the MMP-2 (A), MMP-9 (B), TIMP-1 (C), TIMP-2 (D) mRNA vs 18s mRNA are presented as means ±SEM. *** P<0.001 untreated cells