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Derivation of induced pluripotent stem cell lines from New Zealand donors

Jin Kyo Oha Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand;c Maurice Wilkins Centre, Auckland, New ZealandView further author information

Aneta Przepiorskia Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand

https://orcid.org/0000-0002-4586-0798 View further author information

Hao-Han Changa Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

Rachel C. Dodda Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

Veronika Sandera Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand

https://orcid.org/0000-0002-2918-833X View further author information

Brie Sorrensona Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

Jen-Hsing Shiha Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

Jennifer A. Hollywooda Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

Janak R. de Zoysab Department of Medicine, University of Auckland, Auckland, New Zealand;d Renal Service, Waitemata DHB, Auckland, New Zealand

https://orcid.org/0000-0001-7528-9230 View further author information

Peter R. Shepherda Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand;c Maurice Wilkins Centre, Auckland, New ZealandView further author information

Alan J. Davidsona Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand;c Maurice Wilkins Centre, Auckland, New ZealandCorrespondence[email protected]
View further author information

Teresa M. Holma Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandView further author information

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Supplemental material

Supplementary Figure 5. Generation of TP53-KO iPSC line.

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Supplementary Figure 4. RT-PCR indicating the clearance of the Sendai virus-associated transgenes for MANZ-2-2 (passage number: 9) and MANZ-4-37 (passage number: 8).

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Supplementary Figure 3. STR analysis confirmed that the MANZ-2-2 and MANZ-4-37 iPSC lines are derived from their corresponding fibroblasts. To protect the donors’ identities, only 2 fingerprint markers are shown.

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Supplementary Figure 2. Identification of the most stable reference genes for two different conditions; (A) pluripotent cells and (B) differentiated cells.

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Supplementary Figure 1. Medical information of the fibroblast donors.

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Derivation of induced pluripotent stem cell lines from New Zealand donors

Supplementary Figure 5. Generation of TP53-KO iPSC line.

Supplementary Figure 4. RT-PCR indicating the clearance of the Sendai virus-associated transgenes for MANZ-2-2 (passage number: 9) and MANZ-4-37 (passage number: 8).

Supplementary Figure 3. STR analysis confirmed that the MANZ-2-2 and MANZ-4-37 iPSC lines are derived from their corresponding fibroblasts. To protect the donors’ identities, only 2 fingerprint markers are shown.

Supplementary Figure 2. Identification of the most stable reference genes for two different conditions; (A) pluripotent cells and (B) differentiated cells.

Supplementary Figure 1. Medical information of the fibroblast donors.

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Derivation of induced pluripotent stem cell lines from New Zealand donors

Supplementary Figure 5. Generation of TP53-KO iPSC line.

Supplementary Figure 4. RT-PCR indicating the clearance of the Sendai virus-associated transgenes for MANZ-2-2 (passage number: 9) and MANZ-4-37 (passage number: 8).

Supplementary Figure 3. STR analysis confirmed that the MANZ-2-2 and MANZ-4-37 iPSC lines are derived from their corresponding fibroblasts. To protect the donors’ identities, only 2 fingerprint markers are shown.

Supplementary Figure 2. Identification of the most stable reference genes for two different conditions; (A) pluripotent cells and (B) differentiated cells.

Supplementary Figure 1. Medical information of the fibroblast donors.

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