In vitro inhibition of cytosolic carbonic anhydrases I and II by some new dihydroxycoumarin compounds

Abstract

A new series of 6, 7-dihydroxy-3-(methylphenyl) chromenones, including three new derivatives, i.e. 6,7-dihydroxy-3-(2-methylphenyl)-2H-chromen-2-one (OPC); 6,7-dihydroxy-3-(3-methylphenyl)-2H-chromen-2-one (MPC); 6,7-dihydroxy-3-(4-methylphenyl)-2H-chromen-2-one (PPC) and one previously described, namely 6,7-dihydroxy-3-phenyl-2H-chromen-2-one (DPC), were synthesized. These compounds were investigated as inhibitors of human carbonic anhydrase I (hCA-I) and human carbonic anhydrase II (hCA-II) which had been purified from human erythrocytes on an affinity gel comprised of L-tyrosine-sulfonamide-Sepharose 4B.

Keywords::

• Carbonic anhydrase
• isozymes I and II
• inhibitors
• coumarin
• phenols
• cancer

Acknowledgements

The authors would like to thank Balikesir University Research Center of Applied Sciences (BURCAS/Balikesir,Turkey) for providing the research facilities and also thank to Dr. Malcolm LYON for his invaluable contribution to this paper.