Photometric microplate assay for estimation of the efficacy of paraoxon-inhibited acetylcholinesterase reactivation

Abstract

Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE) using three reactivators for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained using obidoxime where reactivation was near to 80%. Suitability of photometric microplates for following of reactivation procedures is discussed.

Keywords::

• Acetylcholinesterase
• pesticide
• paraoxon
• obidoxime
• pralidoxime
• HI-6
• inhibition
• photometric microplate
• reactivation

Acknowledgements

We would like to thank to the Ministry of Industry and Trade of the Czech Republic for the Project No.2A-1TP1/007