Protein microarrays: high-throughput tools for proteomics

Abstract

Protein microarrays are versatile tools for parallel, miniaturized screening of binding events involving large numbers of immobilized proteins in a time- and cost-effective manner. They are increasingly applied for high-throughput protein analyses in many research areas, such as protein interactions, expression profiling and target discovery. While conventionally made by the spotting of purified proteins, recent advances in technology have made it possible to produce protein microarrays through in situ cell-free synthesis directly from corresponding DNA arrays. This article reviews recent developments in the generation of protein microarrays and their applications in proteomics and diagnostics.

Keywords::

• high throughput
• in situ synthesis
• protein microarray
• proteomics

Financial & competing interests disclosure

The Babraham Institute is an institute of the Biotechnology and Biological Sciences Research Council (BBSRC), UK. This review is an activity of the ProteomeBinders consortium, a Research Infrastructure Coordination Action supported by the EU 6th Framework Programme. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.