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Drug Design, Development and Therapy Volume 13, 2019 - Issue
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Original Research

Effects Of Triptolide On Tooth Movement And Root Resorption In Rats

Fan Yang1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
,
Xu Xia Wang1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;3 Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
,
Dan Ma1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
,
Qun Cui1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
,
De Hua Zheng1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
,
Xiao Can Liu1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China
&
Jun Zhang1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China;2 Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of ChinaCorrespondence[email protected]
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Pages 3963-3975 | Published online: 25 Nov 2019
 
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Abstract

Purpose

The aim of this study was to investigate the effects of triptolide on the tooth movement and root resorption in rats during orthodontic treatment.

Material and methods

A total of 48 male Wistar rats were divided into three groups of 16 each. The right maxillary first molars of rats were drawn mesially by closed coil nickel–titanium spring with a force of 50 g. The two experimental groups received intraperitoneal injections of triptolide for 14 days at a dose of 15 µg/kg/day and 30 µg/kg/day, respectively. The control group received vehicle injections. After 14 days, the rats were humanely killed. The amount of tooth movement was measured. Eight rats from each group were randomly chosen for analysis of the percentage of root resorption area by scanning electron microscopy. For the remaining eight rats in each group, the H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis were performed.

Results

The amount of tooth movement and the ratio of root resorption area were significantly decreased in the triptolide-treated rats. The number of TRAP-positive cells was significantly lower in triptolide-treated groups. Moreover, the expression of nuclear factor kappa B ligand (RANKL) was reduced. In contrast, the expression of osteoprotegerin was significantly up-regulated. In the tension side, the expressions of runt-related transcription factor 2 and osteocalcin were significantly enhanced by triptolide injection.

Conclusion

Triptolide injection could arrest orthodontic tooth movement and reduce root resorption in rats via inhibition of osteoclastogenesis. In addition, triptolide may exert a positive effect on osteoblastogenesis.

Acknowledgments

This work was supported by the Shandong Province Natural Science Foundation (ZR2019MH113).

Disclosure

The authors report no conflicts of interest in this work.

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