Purification and in vitro antioxidant activities of tellurium-containing phycobiliproteins from tellurium-enriched Spirulina platensis

Fang Yang1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China

Ka-Hing Wong2 Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, People’s Republic of China

Yufeng Yang3 Institute of Hydrobiology, College of Life Science and Technology, Jinan University, Guangzhou, People’s Republic of China

Xiaoling Li1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of ChinaCorrespondence[email protected]

Jie Jiang1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China

Wenjie Zheng1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of ChinaCorrespondence[email protected]

Hualian Wu1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China

Tianfeng Chen1 Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China

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Abstract

Tellurium-containing phycocyanin (Te-PC) and allophycocyanin (Te-APC), two organic tellurium (Te) species, were purified from tellurium-enriched Spirulina platensis by a fast protein liquid chromatographic method. It was found that the incorporation of Te into the peptides enhanced the antioxidant activities of both phycobiliproteins. With fractionation by ammonium sulfate precipitation and hydroxylapatite chromatography, Te-PC and Te-APC could be effectively separated with high purity, and Te concentrations were 611.1 and 625.3 μg g⁻¹ protein in Te-PC and Te-APC, respectively. The subunits in the proteins were identified by using MALDI-TOF-TOF mass spectrometry. Te incorporation enhanced the antioxidant activities of both phycobiliproteins, as examined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid assay. Moreover, Te-PC and Te-APC showed dose-dependent protection on erythrocytes against the water-soluble free radical initiator 2,2′-azo(2-asmidinopropane)dihydrochloride-induced hemolysis. In the hepatoprotective model, apoptotic cell death and nuclear condensation induced by tert-butyl hydroperoxide in HepG2 cells was significantly attenuated by Te-PC and Te-APC. Taken together, these results suggest that Te-PC and Te-APC are promising Te-containing proteins with application potential for treatment of diseases related to oxidative stress.

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Acknowledgments

This work was supported by National Science and Technology support program (2012BAD18B01), Natural Science Foundation of China and Guangdong Province, Program for New Century Excellent Talents in University, Research Fund for the Doctoral Program of Higher Education of China, the Fundamental Research Funds for the Central Universities and China Postdoctoral Science Foundation.

Disclosure

The authors declare that there are no conflicts of interest in this work.

Purification and in vitro antioxidant activities of tellurium-containing phycobiliproteins from tellurium-enriched Spirulina platensis

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Purification and in vitro antioxidant activities of tellurium-containing phycobiliproteins from tellurium-enriched Spirulina platensis

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