Controlled release hydrogen sulfide delivery system based on mesoporous silica nanoparticles protects graft endothelium from ischemia–reperfusion injury

Abstract

Hydrogen sulfide (H₂S) functions as a protective gas transmitter in various physiological and pathological processes, but the lack of ideal donors severely hampers the clinical application of H₂S. This study aims to construct a controlled release H₂S donor and evaluate its protective effect on graft endothelium. Mesoporous silica nanoparticles (MSNs) were synthesized using the sol–gel method and loaded with diallyl trisulfide (DATS), an H₂S-releasing agent named DATS-MSN. In vitro experiments showed that DATS-MSN could alleviate endothelial cell inflammation and enhance endothelial cell proliferation and migration. In vivo experiments demonstrated that the apoptosis of graft endothelium was mitigated in the presence of DATS-MSN. Our results indicated that DATS-MSN, releasing H₂S in a controlled release fashion, could serve as an ideal H₂S donor.

Keywords:

• inflammatory response
• rejection
• cellular uptake
• proliferation
• cardiac allograft vasculopathy

Acknowledgments

This work was supported by Natural Science Foundation of China (no 81270326).

Disclosure

The authors report no conflicts of interest in this work.