Hyperthermia mediated by dextran-coated La_0.7Sr_0.3MnO₃ nanoparticles: in vivo studies

Abstract

Purpose

The aim of this study was to evaluate radiofrequency-induced dextran-coated lanthanum strontium manganese oxide nanoparticles-mediated hyperthermia to be used for tumor regression in mice.

Materials and methods

Nanoparticles were injected intra-tumorally in melanoma-bearing C57BL/6J mice and were subjected to radiofrequency treatment.

Results

Hyperthermia treatment significantly inhibited tumor growth (~84%), increased survival (~50%), and reduced tumor proliferation in mice. Histopathological examination demonstrated immense cell death in treated tumors. DNA fragmentation, increased terminal deoxynucleotidyl transferase-dUTP nick end labeling signal, and elevated levels of caspase-3 and caspase-6 suggested apoptotic cell death. Enhanced catalase activity suggested reactive oxygen species-mediated cell death. Enhanced expression of heat shock proteins 70 and 90 in treated tumors suggested the possible development of “antitumor immunity”.

Conclusion

The dextran-coated lanthanum strontium manganese oxide-mediated hyperthermia can be used for the treatment of cancer.

Keywords:

• lanthanum strontium manganese oxide nanoparticles
• melanoma
• tumor regression
• survival
• heat shock proteins
• MRI

View correction statement:

Hyperthermia Mediated by Dextran-Coated La0.7Sr0.3MnO3 Nanoparticles: In vivo Studies [Corrigendum]

Acknowledgments

The authors thank G Mudliar and UA Risbud (Omega Scan Centre, Ratna Hospital, Pune, India) for MRI.

Disclosure

The authors disclose that there is no financial involvement with an organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the paper.