![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based doped and nondoped zinc oxide nanoparticles were activated in daylight that produced reactive oxygen species in the immediate environment. Lipid and protein oxidation did not occur in dark. The major lipid peroxidation derivatives comprised of conjugated dienes, lipid hydroperoxides, and malondialdehyde whereas water, ethane, methanol, and ethanol were found as the end products. Proteins were oxidized to carbonyls, hydroperoxides, and thiol degrading products. Interestingly, lipid hydroperoxides were produced by more than twofold of the protein hydroperoxides, indicating higher degradation of lipids compared to proteins. The in vitro evidence represented a significant contribution of the involvement of both lipid and protein oxidation in the annihilated antipromastigote effect of nanoparticles.
Acknowledgments
The authors thankfully acknowledge Dr Zabta Khan Shinwari (Molecular Systematics and Ethnobotany Lab, Quaid-i-Azam University, Islamabad) for providing some of the lab facilities. The authors are thankful to Mohammad Ghasemi Rad and Tariq Khan for proofreading the manuscript. The L. tropica KWH23 was gifted by Professor Akram Shah, University of Peshawar, Pakistan. The strain was acquired in 2010 during Dr Nazma’s PhD at the London School of Hygiene and Tropical Medicine, UK. The authors are thankful to both Drs Akram Shah and Nazma.
Disclosure
The authors report no conflicts of interest in this work.