Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing

Abstract

In this study, silver nanoparticles (AgNPs) were synthesized for the first time using an antibacterial endophytic fungus of Chinese medicinal herb Orchidantha chinensis, which has anti-inflammatory and antimicrobial activities. The AgNPs were analyzed by various characterization techniques to reveal their morphology, chemical composition, and stability. Also, the relationship between Chinese medicinal herbs, endophytic fungi, and the property of AgNPs was investigated for the first time. Interestingly, an experiment performed in this study revealed the proteins produced by the endophytic fungus to be capped on the nanoparticles, which led to an increase in the stability of spherical and polydispersed AgNPs with low aggregation for over 6 months. More importantly, further study demonstrated that the AgNPs possessed superior antibacterial activity and effectively promoted wound healing. Altogether, the biosynthesis of active AgNPs using the endophytic fungus from Chinese medicinal herb based on the symbiosis theory is simple, eco-friendly, and promising.

Keywords:

• silver nanoparticles
• Orchidantha chinensis
• endophytic fungi
• symbiosis theory
• wound healing

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos 81274097, 81573618), the Pearl River S&T Nova Program of Guangzhou (Grant No 2013J2200059), and the Cultivation Foundation for Distinguished Young Teachers in Higher Education of Guangdong (Grant No Yq2013099).

Disclosure

The authors report no conflicts of interest in this work.