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Abstract
Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression. To overcome this problem, we developed an Adv coated with a spermine-pullulan (SP) cationic polymer and investigated its physicochemical properties and internalization mechanisms. We demonstrated that the SP coating could enhance adenoviral transduction of MSCs without detectable cytotoxicity or effects on differentiation. Our results argue in favor of the potentiality of the SP-coated Adv as a prototype vector for efficient and safe transduction of MSCs.
Acknowledgments
This study was supported in part by grants from the National Natural Science Foundation of China (81101719, 21577167, and 81273441), by Chinese Academy of Sciences (CAS) Strategic Leading Science & Technology Program grant (XDB14040301), the Hundred Talent Program of CAS at the Research Center for Eco-Environmental Sciences, CAS (121311ZXPP2014004), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ([2012]1707).
Disclosure
The authors report no conflicts of interest in this work.