Macrophages participate in local and systemic inflammation induced by amorphous silica nanoparticles through intratracheal instillation

Abstract

Silica nanoparticles (SiNPs) are amongst the most commonly used materials in the field of nanomedicine and, therefore, their influence on organisms has drawn increasing attention in recent years. Most reports have focused on the single tissue reactions induced by SiNPs. Herein, the reaction of primary organs to SiNPs following intratracheal instillation in mice was analyzed by histopathology and ultrastructure observation. Following elucidation of the role of macrophages in local and systemic inflammation, the underlying mechanisms were explored using a macrophage cell line in vitro. The results suggest that macrophages swallow the SiNPs and secrete inflammatory factors by activating the NLRP3 inflammasome, thus participating in local and systemic inflammation.

Keywords:

• silica nanoparticles
• inflammation
• macrophages
• organs
• NLRP3 inflammasome

Acknowledgments

This work was supported by National Natural Science Foundation of China (NSFC) (No 81402709, No 81402710, and No 81202608).

Disclosure

The authors report no conflicts of interest in this work.