Metabolism of 11-Deoxycortisol by cytochrome P450(11β): Identification of reaction products by ¹H-NMR and LC/MS-APCI method.

Miho Ohta Laboratory of Nutrition, Koshien College, 4-25 Kawarabayashi-cho, Nishinomiya, 663

Shigeru Fujii Laboratory of Chemistry, Kansai Medical University, Hirakata, 573

Yasuki Nonaka Department of Molecular Physiological Chemistry, Osaka University Medical School, Suita, 565, Japan

Mitsuhiro Okamoto Department of Molecular Physiological Chemistry, Osaka University Medical School, Suita, 565, Japan

Abstract

A hitherto-unidentified steroid produced in the reaction mixture of 11-deoxycortisol with bovine cytochrome P450(11β) reconstitution system was purified by repeated HPLCs, and subjected to ¹H-NMR analysis. The result suggested that the new metabolite was 19-oxo-11-deoxycortisol. This was further confirmed by a new analytical technique using LC/MS equipped with atmospheric pressure chemical ion (APCI)-analyzer.

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Metabolism of 11-Deoxycortisol by cytochrome P450(11β): Identification of reaction products by ¹H-NMR and LC/MS-APCI method.

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Metabolism of 11-Deoxycortisol by cytochrome P450(11β): Identification of reaction products by 1H-NMR and LC/MS-APCI method.

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Metabolism of 11-Deoxycortisol by cytochrome P450(11β): Identification of reaction products by ¹H-NMR and LC/MS-APCI method.