Evaluation of in vitro effects of some analgesic drugs on erythrocyte and recombinant carbonic anhydrase I and II

Abstract

The in vitro effects of the injectable form of analgesic drugs, dexketoprofen trometamol, dexamethasone sodium phosphate, metamizole sodium, diclofenac sodium, thiocolchicoside, on the activity of purified human carbonic anhydrase I and II were evaluated. The effect of these drugs on erythrocyte hCA I and hCA II was compared to recombinant hCA I and hCA II expressed in Ecoli. IC₅₀ values of the drugs that caused inhibition were determined by means of activity percentage diagrams. The IC₅₀ concentrations of dexketoprofen trometamol and dexamethasone sodium phosphate on hCA I were 683 μM and 4250 μM and for hCA II 950 μM and 6200 μM respectively. Conversely, the enzyme activity was increased by diflofenac sodium. In addition, thiocolchicoside has not any affect on hCA I and hCA II. The effect of these drugs on erythrocyte hCA I and hCA II were consistent with the inhibition of recombinant enzymes.

Keywords::

• Carbonic anhydrase
• analgesic drugs
• inhibition
• IC₅₀

Acknowledgment

The authors thank Ahmet Kuvvetli for his kind help in providing fresh blood samples. hCA II expression plasmid was a gift from David Silverman, USA.

Declaration of interest

The authors report no conflicts of interest.