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Original Article

Analysis by Pulsed-field Gel Electrophoresis of DNA Double-strand Breaks Induced by Heat and/or X-irradiation in Bulk and Replicating DNA of CHO Cells

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Pages 141-152 | Received 24 Jan 1995, Accepted 04 May 1995, Published online: 03 Jul 2009

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Makoto Ihara, Satoshi Takeshita, Kumio Okaichi, Yutaka Okumura & Takeo Ohnishi. (2014) Heat exposure enhances radiosensitivity by depressing DNA-PK kinase activity during double strand break repair. International Journal of Hyperthermia 30:2, pages 102-109.
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Stefan M. Routt, Ju Zhu, Jeffrey M Zaleski & Joseph R Dynlacht. (2011) Potentiation of metalloenediyne cytotoxicity by hyperthermia. International Journal of Hyperthermia 27:5, pages 435-444.
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J. R. Dynlacht, M. Xu, R. K. Pandita, E. A. Wetzel & J. L. Roti Roti. (2004) Effects of heat shock on the Mre11/Rad50/Nbs1 complex in irradiated or unirradiated cells. International Journal of Hyperthermia 20:2, pages 144-156.
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M. W. Dewhirst, M. Lora-Michiels, B. L. Viglianti, W. C. Dewey & M. Repacholi. (2003) Carcinogenic effects of hyperthermia. International Journal of Hyperthermia 19:3, pages 236-251.
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F. HUTCHINSON. (1996) Formation of two double-strand breaks in the same DNA molecule by a single high-energy photon or ionizing particle. International Journal of Radiation Biology 70:5, pages 505-512.
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Liping Bai, Tobias Pfeifer, Wolfgang Gross, Carolina De La Torre, Shuyang Zhao, Li Liu, Michael Schaefer & Ingrid Herr. (2021) Establishment of Tumor Treating Fields Combined With Mild Hyperthermia as Novel Supporting Therapy for Pancreatic Cancer. Frontiers in Oncology 11.
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Joy E. Garrett, Joy E. Garrett, Erin Metzger, Katelyn Schmitt, Sarai Soto, Samantha Northern, Laura Kryah, Misbah Irfan, Susan Rice, Mary Brown, Jeffrey M. Zaleski & Joseph R. Dynlacht. (2019) Enhancement of Cytotoxicity of Enediyne Compounds by Hyperthermia: Effects of Various Metal Complexes on Tumor Cells. Radiation Research 193:2, pages 107.
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Artem K. Velichko, Elena N. Markova, Nadezhda V. Petrova, Sergey V. Razin & Omar L. Kantidze. (2013) Mechanisms of heat shock response in mammals. Cellular and Molecular Life Sciences 70:22, pages 4229-4241.
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Bin Yan, Ruoyun Ouyang, Chenghui Huang, Franklin Liu, Daniel Neill, Chuanyuan Li & Mark Dewhirst. (2012) Heat induces gene amplification in cancer cells. Biochemical and Biophysical Research Communications 427:3, pages 473-477.
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Artem K. Velichko, Nadezhda V. Petrova, Omar L. Kantidze & Sergey V. Razin. (2012) Dual effect of heat shock on DNA replication and genome integrity. Molecular Biology of the Cell 23:17, pages 3450-3460.
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Chenghui Huang, Yaping Li, Peiguo Cao, Zhaoxia Xie & Zhiqiang Qin. (2011) Synergistic effect of hyperthermia and neferine on reverse multidrug resistance in adriamycin-resistant SGC7901/ADM gastric cancer cells. Journal of Huazhong University of Science and Technology [Medical Sciences] 31:4, pages 488-496.
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Joseph L. Roti Roti, Robert P. VanderWaal & Andrei Laszlo. 2011. Molecular Determinants of Radiation Response. Molecular Determinants of Radiation Response 227 249 .
Bogdan I. Gerashchenko, Gerirose Gooding & Joseph R. Dynlacht. (2010) Hyperthermia alters the interaction of proteins of the Mre11 complex in irradiated cells. Cytometry Part A 77A:10, pages 940-952.
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Akihisa TAKAHASHI, Eiichiro MORI & Takeo OHNISHI. (2010) The Foci of DNA Double Strand Break-recognition Proteins Localize with γH2AX after Heat Treatment. Journal of Radiation Research 51:1, pages 91-95.
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Takeo Ohnishi, Eiichiro Mori & Akihisa Takahashi. (2009) DNA double-strand breaks: Their production, recognition, and repair in eukaryotes. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 669:1-2, pages 8-12.
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Wei Wu, Chen Zhang, Zhanghui Chen, Guanglin Zhang & Jun Yang. (2009) Differences in heating methods may account for variation in reported effects on γH2AX focus formation. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 676:1-2, pages 48-53.
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M. Brezeanu, F. Träger & F. Hubenthal. (2009) Scanning force microscopy studies of X-ray-induced double-strand breaks in plasmid DNA. Journal of Biological Physics 35:2, pages 163-174.
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Akihisa Takahashi, Eiichiro Mori, Georgios I. Somakos, Ken Ohnishi & Takeo Ohnishi. (2008) Heat induces γH2AX foci formation in mammalian cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 656:1-2, pages 88-92.
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EIICHIRO MORI, AKIHISA TAKAHASHI & TAKEO OHNISHI. (2008) The Biology of Heat-induced DNA Double-Strand Breaks. Thermal Medicine 24:2, pages 39-50.
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Riadh Habash. 2007. Bioeffects and Therapeutic Applications of Electromagnetic Energy. Bioeffects and Therapeutic Applications of Electromagnetic Energy 199 220 .
Clayton R. Hunt, Raj K. Pandita, Andrei Laszlo, Ryuji Higashikubo, Manjula Agarwal, Tetsuya Kitamura, Arun Gupta, Nicole Rief, Nobuo Horikoshi, Rajeskaran Baskaran, Ji-Hoon Lee, Markus Löbrich, Tanya T. Paull, Joseph L. Roti Roti & Tej K. Pandita. (2007) Hyperthermia Activates a Subset of Ataxia-Telangiectasia Mutated Effectors Independent of DNA Strand Breaks and Heat Shock Protein 70 Status. Cancer Research 67:7, pages 3010-3017.
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Akihisa Takahashi, Eiichiro Mori & Takeo Ohnishi. (2007) Phospho-Nbs1 and Mre11 proteins which recognize DSBs co-localize with γH2AX in the nucleus after heat treatment. Annals of Cancer Research and Therapy 15:2, pages 50-53.
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Ken Ohnishi, Zorica Scuric, David Yau, Robert H. Schiestl, Noritomo Okamoto, Akihisa Takahashi & Takeo Ohnishi. (2006) Heat‐induced phosphorylation of NBS1 in human skin fibroblast cells. Journal of Cellular Biochemistry 99:6, pages 1642-1650.
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AKIHISA TAKAHASHI & TAKEO OHNISHI. (2006) What is the Critical Hyperthermia Target in Cancer Cells?. Thermal Medicine 22:4, pages 229-237.
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Akihisa Takahashi & Takeo Ohnishi. (2005) Does γH2AX foci formation depend on the presence of DNA double strand breaks?. Cancer Letters 229:2, pages 171-179.
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G.P. Raaphorst, J.M. LeBlanc, L.F. Li & D.P. Yang. (2005) Hyperthermia responses in cell lines with normal and deficient DNA repairs systems. Journal of Thermal Biology 30:6, pages 478-484.
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Haruna Kaneko, Kaori Igarashi, Keiko Kataoka & Masahiko Miura. (2005) Heat shock induces phosphorylation of histone H2AX in mammalian cells. Biochemical and Biophysical Research Communications 328:4, pages 1101-1106.
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Akihisa Takahashi, Hideki Matsumoto, Kosuke Nagayama, Mutsuko Kitano, Sayako Hirose, Hidenori Tanaka, Eiichiro Mori, Nobuhiro Yamakawa, Jun-ichi Yasumoto, Kazue Yuki, Ken Ohnishi & Takeo Ohnishi. (2004) Evidence for the Involvement of Double-Strand Breaks in Heat-Induced Cell Killing. Cancer Research 64:24, pages 8839-8845.
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Joshua D. Seno & Joseph R. Dynlacht. (2004) Intracellular redistribution and modification of proteins of the Mre11/Rad50/Nbs1 DNA repair complex following irradiation and heat-shock. Journal of Cellular Physiology 199:2, pages 157-170.
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Masanobu HAYASHI, Taku HAMASU, Daiji ENDOH, Ai KAWANA, Minako MATSUMOTO & Toyo OKUI. (2004) High Sensitivity of Fibroblast Cell Lines Derived from LEC Rats to Heat Treatment. Journal of Veterinary Medical Science 66:10, pages 1177-1182.
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Joseph R. Dynlacht, M. Eric Bittner, Jody A. Bethel & Brian D. Beck. (2003) The non‐homologous end‐joining pathway is not involved in the radiosensitization of mammalian cells by heat shock. Journal of Cellular Physiology 196:3, pages 557-564.
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Koji Orihara, Sadatoshi Biro, Shuichi Hamasaki, Hideyuki Eto, Masaaki Miyata, Yoshiyuki Ikeda & Chuwa Tei. (2002) Hyperthermia at 43°C for 2h Inhibits the Proliferation of Vascular Smooth Muscle Cells, but not Endothelial Cells. Journal of Molecular and Cellular Cardiology 34:9, pages 1205-1215.
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Brian D. Beck & Joseph R. Dynlacht. (2001) Heat-Induced Aggregation of XRCC5 (Ku80) in Nontolerant and Thermotolerant Cells. Radiation Research 156:6, pages 767-774.
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Joseph R. Dynlacht, Zachary V. Roberts, Marvin Earles, Jim Henthorn & Joshua D. Seno. (2000) Different Patterns of DNA Fragmentation and Degradation of Nuclear Matrix Proteins during Apoptosis Induced by Radiation, Hyperthermia or Etoposide 1 . Radiation Research 154:5, pages 515-530.
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MASANORI TOMITA, NORIO SUZUKI, YOSHIHISA MATSUMOTO, KAZUYA HIRANO, NORIKO UMEDA & KAZUO SAKAI. (2000) Sensitization by Wortmannin of Heat- or X-ray Induced Cell Death in Cultured Chinese Hamster V79 Cells. Journal of Radiation Research 41:2, pages 93-102.
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