492
Views
57
CrossRef citations to date
0
Altmetric
Research Article

Primary porcine brain microvascular endothelial cells: Biochemical and functional characterisation as a model for drug transport and targeting

, &
Pages 253-268 | Received 24 Feb 2007, Accepted 06 Apr 2007, Published online: 08 Oct 2008

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (5)

Yadollah Omidi, Nazanin Kianinejad, Young Kwon & Hossein Omidian. (2021) Drug delivery and targeting to brain tumors: considerations for crossing the blood-brain barrier. Expert Review of Clinical Pharmacology 14:3, pages 357-381.
Read now
Abhirup Mandal, Vibhuti Agrahari, Varun Khurana, Dhananjay Pal & Ashim K. Mitra. (2017) Transporter effects on cell permeability in drug delivery. Expert Opinion on Drug Delivery 14:3, pages 385-401.
Read now
Péter Krajcsi, Márton Jani, Beáta Tóth, Franciska Erdő, Emese Kis, Erzsébet Beéry & István Sziráki. (2012) Efflux transporters in the blood–brain interfaces – in vitro and in vivo methods and correlations. Expert Opinion on Drug Metabolism & Toxicology 8:4, pages 419-431.
Read now
Gisele Rodrigues Da Silva, Eliane Ayres, Rodrigo Lambert Orefice, Sandra Aparecida L. Moura, Denise Carmona Cara & Armando Da Silva Cunha$suffix/text()$suffix/text(). (2009) Controlled release of dexamethasone acetate from biodegradable and biocompatible polyurethane and polyurethane nanocomposite. Journal of Drug Targeting 17:5, pages 374-383.
Read now
Jaleh Barar, Ali Reza Javadzadeh & Yadollah Omidi. (2008) Ocular novel drug delivery: impacts of membranes and barriers. Expert Opinion on Drug Delivery 5:5, pages 567-581.
Read now

Articles from other publishers (52)

Adélie Mellinger, Larissa J. Lubitz, Claire Gazaille, Gero Leneweit, Guillaume Bastiat, Claire Lépinoux-Chambaud & Joël Eyer. (2023) The use of liposomes functionalized with the NFL-TBS.40–63 peptide as a targeting agent to cross the in vitro blood–brain barrier and target glioblastoma cells. International Journal of Pharmaceutics 646, pages 123421.
Crossref
Verena Ledwig & Stephan Reichl. (2023) Isolation and Cultivation of Porcine Endothelial Cells, Pericytes and Astrocytes to Develop an In Vitro Blood–Brain Barrier Model for Drug Permeation Testing. Pharmaceutics 15:6, pages 1688.
Crossref
Andrzej Łach, Agnieszka Wnuk & Anna Katarzyna Wójtowicz. (2023) Experimental Models to Study the Functions of the Blood–Brain Barrier. Bioengineering 10:5, pages 519.
Crossref
N. Joan Abbott, Siti R. Yusof, Andreas Reichel, Diana E. M. Dolman & Jane E. Preston. 2022. Drug Delivery to the Brain. Drug Delivery to the Brain 211 254 .
Simona Federica Spampinato, Yukio Takeshita & Birgit Obermeier. 2022. The Blood-Brain Barrier. The Blood-Brain Barrier 333 352 .
Carolin J. Curtaz, Constanze Schmitt, Kinga G. Blecharz-Lang, Norbert Roewer, Achim Wöckel & Malgorzata Burek. (2020) Circulating MicroRNAs and Blood-Brain-Barrier Function in Breast Cancer Metastasis. Current Pharmaceutical Design 26:13, pages 1417-1427.
Crossref
Yoichi Morofuji & Shinsuke Nakagawa. (2020) Drug Development for Central Nervous System Diseases Using In vitro Blood-brain Barrier Models and Drug Repositioning. Current Pharmaceutical Design 26:13, pages 1466-1485.
Crossref
Maryam H. Shubbar & Jeffrey I. Penny. (2020) Therapeutic drugs modulate ATP-Binding cassette transporter-mediated transport of amyloid beta(1–42) in brain microvascular endothelial cells. European Journal of Pharmacology 874, pages 173009.
Crossref
Lina Bergman, Pablo Torres-Vergara, Jeffrey Penny, Johan Wikström, Maria Nelander, Jose Leon, Mary Tolcher, James M. Roberts, Anna-Karin Wikström & Carlos Escudero. (2019) Investigating Maternal Brain Alterations in Preeclampsia: the Need for a Multidisciplinary Effort. Current Hypertension Reports 21:9.
Crossref
Bhupesh Sharma, Kanishk Luhach & G.T. Kulkarni. 2019. Brain Targeted Drug Delivery System. Brain Targeted Drug Delivery System 53 101 .
Keith D. Rochfort & Philip M. Cummins. 2019. Blood-Brain Barrier. Blood-Brain Barrier 71 98 .
Maryam H. Shubbar & Jeffrey I. Penny. (2018) Effect of amyloid beta on ATP-binding cassette transporter expression and activity in porcine brain microvascular endothelial cells. Biochimica et Biophysica Acta (BBA) - General Subjects 1862:10, pages 2314-2322.
Crossref
Hassan Pezeshgi Modarres, Mohsen Janmaleki, Mana Novin, John Saliba, Fatima El-Hajj, Mahdi RezayatiCharan, Amir Seyfoori, Hamid Sadabadi, Milène Vandal, Minh Dang Nguyen, Anwarul Hasan & Amir Sanati-Nezhad. (2018) In vitro models and systems for evaluating the dynamics of drug delivery to the healthy and diseased brain. Journal of Controlled Release 273, pages 108-130.
Crossref
Isobel ParkesSatyan ChintawarM. Zameel Cader. (2018) Neurovascular dysfunction in dementia – human cellular models and molecular mechanisms. Clinical Science 132:3, pages 399-418.
Crossref
S. Lelu, M. Afadzi, S. Berg, A. K. O. Aslund, S. H. Torp, W. Sattler & C. de L. Davies. (2017) Primary Porcine Brain Endothelial Cells as In Vitro Model to Study Effects of Ultrasound and Microbubbles on Blood–Brain Barrier Function . IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control 64:1, pages 281-290.
Crossref
Asli Kara, Naile Ozturk & Imran Vural. 2017. Nanotechnology Methods for Neurological Diseases and Brain Tumors. Nanotechnology Methods for Neurological Diseases and Brain Tumors 151 185 .
Jaleh Barar, Mohammad A. Rafi, Mohammad M. Pourseif & Yadollah Omidi. (2016) Blood-brain barrier transport machineries and targeted therapy of brain diseases. BioImpacts 6:4, pages 225-248.
Crossref
Hannah K. WilsonMadeline G. FaubionMichael K. HjortnessSean P. PalecekEric V. Shusta. (2016) Cryopreservation of Brain Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Is Enhanced by Rho-Associated Coiled Coil-Containing Kinase Inhibition. Tissue Engineering Part C: Methods 22:12, pages 1085-1094.
Crossref
Juan Aparicio-Blanco, Cristina Martín-Sabroso & Ana-Isabel Torres-Suárez. (2016) In vitro screening of nanomedicines through the blood brain barrier: A critical review. Biomaterials 103, pages 229-255.
Crossref
Hiroyuki OhshimaYung‐Chih Kuo, Chin‐Lung Lee & Jyh‐Ping Hsu. 2016. Encyclopedia of Biocolloid and Biointerface Science 2V Set. Encyclopedia of Biocolloid and Biointerface Science 2V Set 244 254 .
Jayati Banerjee, Yejiao Shi & Helena S. Azevedo. (2016) In vitro blood–brain barrier models for drug research: state-of-the-art and new perspectives on reconstituting these models on artificial basement membrane platforms. Drug Discovery Today 21:9, pages 1367-1386.
Crossref
Marijke De Bock, Valérie Van Haver, Roosmarijn E. Vandenbroucke, Elke Decrock, Nan Wang & Luc Leybaert. (2016) Into rather unexplored terrain-transcellular transport across the blood-brain barrier. Glia 64:7, pages 1097-1123.
Crossref
Hans C Helms, N Joan Abbott, Malgorzata Burek, Romeo Cecchelli, Pierre-Olivier Couraud, Maria A Deli, Carola Förster, Hans J Galla, Ignacio A Romero, Eric V Shusta, Matthew J Stebbins, Elodie Vandenhaute, Babette Weksler & Birger Brodin. (2016) In vitro models of the blood–brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow & Metabolism 36:5, pages 862-890.
Crossref
Jaleh Barar, Ayuob Aghanejad, Marziyeh Fathi & Yadollah Omidi. (2016) Advanced drug delivery and targeting technologies for the ocular diseases. BioImpacts 6:1, pages 49-67.
Crossref
Ghaith Al-Jayyoussi, Daniel F. Price, Katharina Kreitmeyr, John P. Keogh, Mathew W. Smith, Mark Gumbleton & Christopher J. Morris. (2015) Absorption of ipratropium and l -carnitine into the pulmonary circulation of the ex-vivo rat lung is driven by passive processes rather than active uptake by OCT/OCTN transporters. International Journal of Pharmaceutics 496:2, pages 834-841.
Crossref
Anette Wolff, Maria Antfolk, Birger Brodin & Maria Tenje. (2015) In Vitro Blood–Brain Barrier Models—An Overview of Established Models and New Microfluidic Approaches. Journal of Pharmaceutical Sciences 104:9, pages 2727-2746.
Crossref
Louiza Bohn Thomsen, Annette Burkhart & Torben Moos. (2015) A Triple Culture Model of the Blood-Brain Barrier Using Porcine Brain Endothelial cells, Astrocytes and Pericytes. PLOS ONE 10:8, pages e0134765.
Crossref
David J. Mc Carthy, Meenakshi Malhotra, Aoife M. O’Mahony, John F. Cryan & Caitriona M. O’Driscoll. (2014) Nanoparticles and the Blood-Brain Barrier: Advancing from In-Vitro Models Towards Therapeutic Significance. Pharmaceutical Research 32:4, pages 1161-1185.
Crossref
Alex Avdeef, Mária A. Deli & Winfried Neuhaus. 2015. Blood-Brain Barrier in Drug Discovery. Blood-Brain Barrier in Drug Discovery 188 237 .
Yarong HeYao YaoStella E. TsirkaYu Cao. (2014) Cell-Culture Models of the Blood–Brain Barrier. Stroke 45:8, pages 2514-2526.
Crossref
Joana Bicker, Gilberto Alves, Ana Fortuna & Amílcar Falcão. (2014) Blood–brain barrier models and their relevance for a successful development of CNS drug delivery systems: A review. European Journal of Pharmaceutics and Biopharmaceutics 87:3, pages 409-432.
Crossref
P Marc D Watson, Judy C Paterson, George Thom, Ulrika Ginman, Stefan Lundquist & Carl I Webster. (2013) Modelling the endothelial blood-CNS barriers: a method for the production of robust in vitromodels of the rat blood-brain barrier and blood-spinal cord barrier. BMC Neuroscience 14:1.
Crossref
Céline Passeleu-Le Bourdonnec, Pierre-Alain Carrupt, Jean Michel Scherrmann & Sophie Martel. (2013) Methodologies to Assess Drug Permeation Through the Blood–Brain Barrier for Pharmaceutical Research. Pharmaceutical Research 30:11, pages 2729-2756.
Crossref
Adjanie Patabendige, Robert A. Skinner, Louise Morgan & N. Joan Abbott. (2013) A detailed method for preparation of a functional and flexible blood–brain barrier model using porcine brain endothelial cells. Brain Research 1521, pages 16-30.
Crossref
Adjanie Patabendige, Robert A. Skinner & N. Joan Abbott. (2013) Establishment of a simplified in vitro porcine blood–brain barrier model with high transendothelial electrical resistance. Brain Research 1521, pages 1-15.
Crossref
David Dickens, Siti R. Yusof, N. Joan Abbott, Babette Weksler, Ignacio A. Romero, Pierre-Olivier Couraud, Ana Alfirevic, Munir Pirmohamed & Andrew Owen. (2013) A Multi-System Approach Assessing the Interaction of Anticonvulsants with P-gp. PLoS ONE 8:5, pages e64854.
Crossref
Yadollah Omidi, Jaleh Barar & Hossein Hamzeiy. 2012. Nanotechnology in Health Care. Nanotechnology in Health Care 43 106 .
Carina A. Cantrill, Robert A. Skinner, Nancy J. Rothwell & Jeffrey I. Penny. (2012) An immortalised astrocyte cell line maintains the in vivo phenotype of a primary porcine in vitro blood–brain barrier model. Brain Research 1479, pages 17-30.
Crossref
Yukio Takeshita & Richard M. Ransohoff. (2012) Inflammatory cell trafficking across the blood-brain barrier: chemokine regulation and in vitro models. Immunological Reviews 248:1, pages 228-239.
Crossref
Sylvia Van Dorpe, Antoon Bronselaer, Joachim Nielandt, Sofie Stalmans, Evelien Wynendaele, Kurt Audenaert, Christophe Van De Wiele, Christian Burvenich, Kathelijne Peremans, Hung Hsuchou, Guy De Tré & Bart De Spiegeleer. (2011) Brainpeps: the blood–brain barrier peptide database. Brain Structure and Function 217:3, pages 687-718.
Crossref
Flávia Carmo Horta Pinto, Armando Da Silva-Cunha Junior, Rodrigo Lambert Oréfice, Eliane Ayres, Silvia Passos Andrade, Luiza Dias C. Lima, Sandra A. Lima Moura & Gisele Rodrigues Da Silva. (2012) Controlled release of triamcinolone acetonide from polyurethane implantable devices: application for inhibition of inflammatory-angiogenesis. Journal of Materials Science: Materials in Medicine 23:6, pages 1431-1445.
Crossref
Alex Avdeef. 2012. Absorption and Drug Development. Absorption and Drug Development 575 680 .
Alex Avdeef. (2011) How well can in vitro brain microcapillary endothelial cell models predict rodent in vivo blood–brain barrier permeability?. European Journal of Pharmaceutical Sciences 43:3, pages 109-124.
Crossref
Christopher J. Morris, Mathew W. Smith, Peter C. Griffiths, Neil B. McKeown & Mark Gumbleton. (2011) Enhanced pulmonary absorption of a macromolecule through coupling to a sequence-specific phage display-derived peptide. Journal of Controlled Release 151:1, pages 83-94.
Crossref
Flávia Carmo Horta Pinto, Armando Silva-Cunha, Gerson Antônio Pianetti, Eliane Ayres, Rodrigo Lambert Oréfice & Gisele Rodrigues Da Silva. (2011) Montmorillonite Clay-Based Polyurethane Nanocomposite As Local Triamcinolone Acetonide Delivery System. Journal of Nanomaterials 2011, pages 1-11.
Crossref
Filipa Lourenço Cardoso, Dora Brites & Maria Alexandra Brito. (2010) Looking at the blood–brain barrier: Molecular anatomy and possible investigation approaches. Brain Research Reviews 64:2, pages 328-363.
Crossref
Amin A. Nomeir. 2010. Early Drug Development. Early Drug Development 25 88 .
Pergentino Balbuena, Wen Li, Geraldine Magnin-Bissel, J. Blair Meldrum & Marion Ehrich. (2010) Comparison of Two Blood-Brain Barrier In Vitro Systems: Cytotoxicity and Transfer Assessments of Malathion/Oxon and Lead Acetate. Toxicological Sciences 114:2, pages 260-271.
Crossref
David J. Brayden, Emilie J. M. Oudot & Alan W. Baird. 2010. Comparative and Veterinary Pharmacology. Comparative and Veterinary Pharmacology 79 112 .
W. Neuhaus, B. Germann, V.E. Plattner, F. Gabor, M. Wirth & C.R. Noe. (2009) Alteration of the glycocalyx of two blood-brain barrier mimicking cell lines is inducible by glioma conditioned media. Brain Research 1279, pages 82-89.
Crossref
Jaleh Barar & Yadollah Omidi. (2008) Bioelectrical and Permeability Properties of Brain Microvasculature Endothelial Cells: Effects of Tight Junction Modulators. Journal of Biological Sciences 8:3, pages 556-562.
Crossref
Olga C. Colgan, Nora T. Collins, Gail Ferguson, Ronan P. Murphy, Yvonne A. Birney, Paul A. Cahill & Philip M. Cummins. (2008) Influence of basolateral condition on the regulation of brain microvascular endothelial tight junction properties and barrier function. Brain Research 1193, pages 84-92.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.