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Expert Opinion on Therapeutic Targets Volume 10, 2006 - Issue 1
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Technology Evaluation

Transcreener™: screening enzymes involved in covalent regulation

Robert G Lowery President and CEO, BellBrook Labs, 525 Science Drive, Suite 110, Madison, WI 53711, USA. [email protected]
&
Karen Kleman-Leyer Project Leader, BellBrook Labs, 525 Science Drive, Suite 110, Madison, WI 53711, USA. [email protected]
Pages 179-190 | Published online: 27 Jan 2006

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Kun Bi, Connie S Lebakken & Kurt W Vogel. (2011) Transformation of in vitro tools for kinase profiling: keeping an eye over the off-target liabilities. Expert Opinion on Drug Discovery 6:7, pages 701-712.
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Wendy A Lea & Anton Simeonov. (2011) Fluorescence polarization assays in small molecule screening. Expert Opinion on Drug Discovery 6:1, pages 17-32.
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Yong Jia. (2008) Current status of HTRF® technology in kinase assays. Expert Opinion on Drug Discovery 3:12, pages 1461-1474.
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Yong Jia, Christopher M Quinn & Robert V Talanian. (2007) Designing in vitro assays for drug discovery: a study with the human MAP3 kinase COT. Expert Opinion on Drug Discovery 2:7, pages 909-915.
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Renata Kasprzyk & Jacek Jemielity. (2021) Enzymatic Assays to Explore Viral mRNA Capping Machinery. ChemBioChem 22:23, pages 3236-3253.
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Scott A. Busby, Seth Carbonneau, John Concannon, Christoph E. Dumelin, YounKyoung Lee, Shin Numao, Nicole Renaud, Thomas M. Smith & Douglas S. Auld. (2020) Advancements in Assay Technologies and Strategies to Enable Drug Discovery. ACS Chemical Biology 15:10, pages 2636-2648.
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Sebastián A. Díaz, Guillermo Lasarte-Aragonés, Robert Lowery, James Vranish, William Klein, Kimihiro Susumu & Igor Medintz. (2019) Time-gated FRET from terbium labeled antibodies to quantum dot acceptors for broad ADP sensing. Time-gated FRET from terbium labeled antibodies to quantum dot acceptors for broad ADP sensing.
G. Singh, M. Kaur, B. A. Shiekh & T. S. Kang. (2018) Luminescent micellar nano-interfaces of surface active ionic liquid for the selective recognition of ADP in aqueous medium. Chemical Communications 54:54, pages 7463-7466.
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Eun Ju Kim. (2017) In Vitro Biochemical Assays for O-GlcNAc-Processing Enzymes. ChemBioChem 18:15, pages 1462-1472.
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Meera Kumar & Robert G. Lowery. (2017) A High-Throughput Method for Measuring Drug Residence Time Using the Transcreener ADP Assay. SLAS Discovery 22:7, pages 915-922.
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Nandu Thrithamarassery Gangadharan, Ananda Baskaran Venkatachalam & Shiburaj Sugathan. 2017. Bioresources and Bioprocess in Biotechnology. Bioresources and Bioprocess in Biotechnology 247 273 .
Thomas ZielinskiMelvin ReichmanPreston S. DonoverRobert G. Lowery. (2016) Development and Validation of a Universal High-Throughput UDP-Glycosyltransferase Assay with a Time-Resolved FRET Signal. ASSAY and Drug Development Technologies 14:4, pages 240-251.
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Jochen Schmid, Dominik Heider, Norma J. Wendel, Nadine Sperl & Volker Sieber. (2016) Bacterial Glycosyltransferases: Challenges and Opportunities of a Highly Diverse Enzyme Class Toward Tailoring Natural Products. Frontiers in Microbiology 7.
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Yusuke Kamiyoshihara, Denise M. Tieman & Harry J. Klee. 2016. Plant Signal Transduction. Plant Signal Transduction 199 207 .
Kari Kopra & Harri Härmä. (2015) Quenching resonance energy transfer (QRET): a single-label technique for inhibitor screening and interaction studies. New Biotechnology 32:6, pages 575-580.
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Feihu Huang, Gefei Hao, Fengxu Wu & Guoqiang Feng. (2015) Fluorescence sensing of ADP over ATP and PPi in 100% aqueous solution. The Analyst 140:17, pages 5873-5876.
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Amelie Fiene, Younis Baqi, Joanna Lecka, Jean Sévigny & Christa E. Müller. (2015) Fluorescence polarization immunoassays for monitoring nucleoside triphosphate diphosphohydrolase (NTPDase) activity. The Analyst 140:1, pages 140-148.
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Lei Shi, Ping Hu, Yanliang Ren & Guoqiang Feng. (2013) An unexpected highly selective mononuclear zinc complex for adenosine diphosphate (ADP). Chemical Communications 49:99, pages 11704.
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Alexander V. LyubimovPeter L. Bullock & Olga V. Trubetskoy. 2011. Encyclopedia of Drug Metabolism and Interactions. Encyclopedia of Drug Metabolism and Interactions 1 26 .
Tony A. Klink, Matt Staeben, Kim Twesten, Andrew L. Kopp, Meera Kumar, Rebecca Schall Dunn, Cori A. Pinchard, Karen M. Kleman-Leyer, Martin Klumpp & Robert G. Lowery. (2012) Development and Validation of a Generic Fluorescent Methyltransferase Activity Assay Based on the Transcreener AMP/GMP Assay. SLAS Discovery 17:1, pages 59-70.
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Gerd K. Wagner & Thomas Pesnot. (2010) Glycosyltransferases and their Assays. ChemBioChem 11:14, pages 1939-1949.
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Matt StaebenKaren M. Kleman-LeyerAndrew L. KoppThane A. WestermeyerRobert G. Lowery. (2010) Development and Validation of a Transcreener Assay for Detection of AMP- and GMP-Producing Enzymes. ASSAY and Drug Development Technologies 8:3, pages 339-350.
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Simone Kunzelmann & Martin R. Webb. (2010) A Fluorescent, Reagentless Biosensor for ADP Based on Tetramethylrhodamine-Labeled ParM. ACS Chemical Biology 5:4, pages 415-425.
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Olga Trubetskoy, Moshe Finel & Vladimir Trubetskoy. (2008) High-throughput screening technologies for drug glucuronidation profiling. Journal of Pharmacy and Pharmacology 60:8, pages 1061-1067.
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Thomas Zielinski, Adam J. Kimple, Stephanie Q. Hutsell, Mark D. Koeff, David P. Siderovski & Robert G. Lowery. (2009) Two Gαi1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity. SLAS Discovery 14:10, pages 1195-1206.
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Hicham ZegzoutiMarina ZdanovskaiaKevin HsiaoSaid A. Goueli. (2009) ADP-Glo: A Bioluminescent and Homogeneous ADP Monitoring Assay for Kinases. ASSAY and Drug Development Technologies 7:6, pages 560-572.
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Hu LiRachel D. TotoritisLeng A. LorBenjamin SchwartzPeter CaprioliAnthony J. JurewiczGuofeng Zhang. (2009) Evaluation of an Antibody-Free ADP Detection Assay: ADP-Glo. ASSAY and Drug Development Technologies 7:6, pages 598-605.
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Simone Kunzelmann & Martin R. Webb. (2009) A Biosensor for Fluorescent Determination of ADP with High Time Resolution. Journal of Biological Chemistry 284:48, pages 33130-33138.
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Lin Hong, Christopher M. Quinn & Yong Jia. (2009) Evaluating the utility of the HTRF® Transcreener™ ADP assay technology: A comparison with the standard HTRF assay technology. Analytical Biochemistry 391:1, pages 31-38.
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Michael Habig, Anke Blechschmidt, Sigmar Dressler, Barbara Hess, Viral Patel, Andreas Billich, Christian Ostermeier, David Beer & Martin Klumpp. (2009) Efficient Elimination of Nonstoichiometric Enzyme Inhibitors from HTS Hit Lists. SLAS Discovery 14:6, pages 679-689.
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Douglas S. Auld, Ya-Qin Zhang, Noel T. Southall, Ganesha Rai, Marc Landsman, Jennifer MacLure, Daniel Langevin, Craig J. Thomas, Christopher P. Austin & James Inglese. (2009) A Basis for Reduced Chemical Library Inhibition of Firefly Luciferase Obtained from Directed Evolution. Journal of Medicinal Chemistry 52:5, pages 1450-1458.
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Karen M. Kleman-LeyerTony A. KlinkAndrew L. KoppThane A. WestermeyerMark D. KoeffBrad R. LarsonTracy J. WorzellaCori A. PinchardSebastianus A.T. van de KarGuido J.R. ZamanJorrit J. HornbergRobert G. Lowery. (2009) Characterization and Optimization of a Red-Shifted Fluorescence Polarization ADP Detection Assay. ASSAY and Drug Development Technologies 7:1, pages 56-67.
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Tony A. Klink, Karen M. Kleman-Leyer, Andrew Kopp, Thane A. Westermeyer & Robert G. Lowery. (2008) Evaluating PI3 Kinase Isoforms Using Transcreener™ ADP Assays. SLAS Discovery 13:6, pages 476-485.
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James Inglese, Ronald L Johnson, Anton Simeonov, Menghang Xia, Wei Zheng, Christopher P Austin & Douglas S Auld. (2007) High-throughput screening assays for the identification of chemical probes. Nature Chemical Biology 3:8, pages 466-479.
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Karen L. Huss, Pauline E. Blonigen & Robert M. Campbell. (2007) Development of a Transcreener™ Kinase Assay for Protein Kinase A and Demonstration of Concordance of Data with a Filter-Binding Assay Format. SLAS Discovery 12:4, pages 578-584.
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Yichin LiuLeeanne ZalamedaKi Won KimMinghan WangJohn D. McCarter. (2007) Discovery of Acetyl-Coenzyme A Carboxylase 2 Inhibitors: Comparison of a Fluorescence Intensity-Based Phosphate Assay and a Fluorescence Polarization-Based ADP Assay for High-Throughput Screening. ASSAY and Drug Development Technologies 5:2, pages 225-236.
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