4,728
Views
68
CrossRef citations to date
0
Altmetric
Reviews

The workflow of single-cell expression profiling using quantitative real-time PCR

&

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (5)

Ismail Abdullahi & Tracy Lawrence. (2022) Accelerated in vitro thermotherapy and indexing against apple chlorotic leaf spot virus in Shiro plum. Canadian Journal of Plant Pathology 44:1, pages 136-146.
Read now
Andrei Cristian Ionescu, Eugenio Brambilla, Lamberto Manzoli, Giovanna Orsini, Valentina Gentili & Roberta Rizzo. (2021) Aerosols modification with H2O2 reduces airborne contamination by dental handpieces. Journal of Oral Microbiology 13:1.
Read now
Margaret Dellett & David Arthur Simpson. (2016) Considerations for optimization of microRNA PCR assays for molecular diagnosis. Expert Review of Molecular Diagnostics 16:4, pages 407-414.
Read now
Monica Sager, Nai Chien Yeat, Stefan Pajaro-Van der Stadt, Charlotte Lin, Qiuyin Ren & Jimmy Lin. (2015) Transcriptomics in cancer diagnostics: developments in technology, clinical research and commercialization. Expert Review of Molecular Diagnostics 15:12, pages 1589-1603.
Read now
Daniel Andersson, Nina Akrap, David Svec, Tony E Godfrey, Mikael Kubista, Göran Landberg & Anders Ståhlberg. (2015) Properties of targeted preamplification in DNA and cDNA quantification. Expert Review of Molecular Diagnostics 15:8, pages 1085-1100.
Read now

Articles from other publishers (63)

Feng-Ming Hsu, Yih-Leong Chang, Chung-Yung Chen, Shu-Rung Lin & Jason Chia-Hsien Cheng. (2023) Hybridization Protection Reaction for Sensitive and Robust Gene Expression Profiling of Clinical Formalin-Fixed Paraffin-Embedded Samples. Clinical Chemistry 69:12, pages 1385-1395.
Crossref
Bo Zhao, Tomonori Fujita, Yoshiaki Nihei, Zaizhi Yu, Xiaohan Chen, Hiroaki Tanaka & Masaru Ihara. (2023) Tracking community infection dynamics of COVID-19 by monitoring SARS-CoV-2 RNA in wastewater, counting positive reactions by qPCR. Science of The Total Environment 904, pages 166420.
Crossref
Katarzyna Linkowska, Tomasz Bogiel, Katarzyna Lamperska, Andrzej Marszałek, Jarosław Starzyński, Łukasz Szylberg, Aleksandra Szwed-Kowalska, Małgorzata Pawłowska & Tomasz Grzybowski. (2023) Commercially available SARS-CoV-2 RT-qPCR diagnostic tests need obligatory internal validation. Scientific Reports 13:1.
Crossref
Dongdong Zhang, Zhenfan Yu, Shaobo Hu, Xingyue Liu, Bin Zeng, Wenwen Gao, HuanXue Qin, Xintong Ma & Yawen He. (2023) Genome-wide identification of members of the Skp1 family in almond (Prunus dulcis), cloning and expression characterization of PsdSSK1. Physiology and Molecular Biology of Plants 29:1, pages 35-49.
Crossref
Maryam Moarefian, Antonia McDonnell CaposselaRyan EomKiana Aran. (2022) Single-Cell Technologies: Advances in Single-Cell Migration and Multi-Omics. GEN Biotechnology 1:3, pages 246-261.
Crossref
Chamteut Oh, Palash Sashittal, Aijia Zhou, Leyi Wang, Mohammed El-Kebir & Thanh H. Nguyen. (2022) Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics. Applied and Environmental Microbiology 88:7.
Crossref
Rhonda Bacher, Li-Fang Chu, Cara Argus, Jennifer M Bolin, Parker Knight, James A Thomson, Ron Stewart & Christina Kendziorski. (2022) Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization. Nucleic Acids Research 50:2, pages e12-e12.
Crossref
Yoichiro Shibuya, Akishige Hokugo, Hiroko Okawa, Takeru Kondo, Daniel Khalil, Lixin Wang, Yvonne Roca, Adam Clements, Hodaka Sasaki, Ella Berry, Ichiro Nishimura & Reza Jarrahy. (2022) Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing. eLife 11.
Crossref
Daniel Zucha, Mikael Kubista & Lukas Valihrach. (2021) Tutorial: Guidelines for Single-Cell RT-qPCR. Cells 10:10, pages 2607.
Crossref
Matthias Mäurer, Katharina Pachmann, Thomas Wendt, Dorothea Schott & Andrea Wittig. (2021) Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients. Current Oncology 28:5, pages 3507-3524.
Crossref
Andrei Cristian Ionescu, Eugenio Brambilla, Lamberto Manzoli, Giovanna Orsini, Valentina Gentili & Roberta Rizzo. (2021) Efficacy of personal protective equipment against coronavirus transmission via dental handpieces. The Journal of the American Dental Association 152:8, pages 631-640.
Crossref
Xavier Fernandez-Cassi, Andreas Scheidegger, Carola Bänziger, Federica Cariti, Alex Tuñas Corzon, Pravin Ganesanandamoorthy, Joseph C. Lemaitre, Christoph Ort, Timothy R. Julian & Tamar Kohn. (2021) Wastewater monitoring outperforms case numbers as a tool to track COVID-19 incidence dynamics when test positivity rates are high. Water Research 200, pages 117252.
Crossref
Deniz Yılmaz & Mustafa Culha. (2021) Investigation of the pathway dependent endocytosis of gold nanoparticles by surface-enhanced Raman scattering. Talanta 225, pages 122071.
Crossref
Rein Brys, David Halfmaerten, Sabrina Neyrinck, Quentin Mauvisseau, Johan Auwerx, Michael Sweet & Joachim Mergeay. (2020) Reliable eDNA detection and quantification of the European weather loach ( Misgurnus fossilis ) . Journal of Fish Biology 98:2, pages 399-414.
Crossref
M.M. Chulkina, A.V. Pichugin & R.I. Ataullakhanov. (2020) Pharmaceutical grade synthetic peptide Thr-Glu-Lys-Lys-Arg-Arg-Glu-Thr-Val-Glu-Arg-Glu-Lys-Glu ameliorates DSS-induced murine colitis by reducing the number and pro-inflammatory activity of colon tissue-infiltrating Ly6G+ granulocytes and Ly6C+ monocytes. Peptides 132, pages 170364.
Crossref
Jane Ru Choi. (2020) Advances in single cell technologies in immunology. BioTechniques 69:3, pages 226-236.
Crossref
Elzbieta Stepula, Xin-Ping Wang, Supriya Srivastav, Matthias König, Janina Levermann, Sabine Kasimir-Bauer & Sebastian Schlücker. (2020) 6-Color/1-Target Immuno-SERS Microscopy on the Same Single Cancer Cell. ACS Applied Materials & Interfaces 12:29, pages 32321-32327.
Crossref
Kenta Shinha, Wataru Nihei & Hiroshi Kimura. (2020) A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells. Micromachines 11:7, pages 691.
Crossref
Daniel J. Browne, Jamie L. Brady, Ashley J. Waardenberg, Claire Loiseau & Denise L. Doolan. (2020) An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells. Frontiers in Immunology 11.
Crossref
Emma Jonasson, Lisa Andersson, Soheila Dolatabadi, Salim Ghannoum, Pierre Åman & Anders Ståhlberg. (2020) Total mRNA Quantification in Single Cells: Sarcoma Cell Heterogeneity. Cells 9:3, pages 759.
Crossref
Patrick Hardinge & James A. H. Murray. (2020) Full Dynamic Range Quantification using Loop-mediated Amplification (LAMP) by Combining Analysis of Amplification Timing and Variance between Replicates at Low Copy Number. Scientific Reports 10:1.
Crossref
Daniel Zucha, Peter Androvic, Mikael Kubista & Lukas Valihrach. (2020) Performance Comparison of Reverse Transcriptases for Single-Cell Studies. Clinical Chemistry 66:1, pages 217-228.
Crossref
Nenad R. Filipović, Snežana K. Bjelogrlić, Sveva Pelliccia, Vesna B. Jovanović, Milan Kojić, Milan Senćanski, Giuseppe La Regina, Romano Silvestri, Christian D. Muller & Tamara R. Todorović. (2020) Selenotriapine – An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors. Arabian Journal of Chemistry 13:1, pages 1466-1489.
Crossref
Ehsan Hajiramezanali, Mahdi Imani, Ulisses Braga-Neto, Xiaoning Qian & Edward R. Dougherty. (2019) Scalable optimal Bayesian classification of single-cell trajectories under regulatory model uncertainty. BMC Genomics 20:S6.
Crossref
Emmanuel Jouen, Fréderic Chiroleu, Véronique Maillot-Lebon, Aude Chabirand, Sabine Merion, Claudine Boyer, Olivier Pruvost & Isabelle Robène. (2019) A duplex quantitative real-time PCR assay for the detection and quantification of Xanthomonas phaseoli pv. dieffenbachiae from diseased and latently infected anthurium tissue. Journal of Microbiological Methods 161, pages 74-83.
Crossref
Kapil Manglani, Viji Vijayan, Chandramani Pathak, Mayuri Khandelwal, Parminder Singh, Stalin Chellappa, Vijay K. Yadav, Avadhesha Surolia & Sarika Gupta. (2019) Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process. Bone 122, pages 123-135.
Crossref
Deniz Yaşar Öztaş, Mine Altunbek, Deniz Uzunoglu, Hülya Yılmaz, Demet Çetin, Zekiye Suludere & Mustafa Çulha. (2019) Tracing Size and Surface Chemistry-Dependent Endosomal Uptake of Gold Nanoparticles Using Surface-Enhanced Raman Scattering. Langmuir 35:11, pages 4020-4028.
Crossref
Min Li & Robbyn K. Anand. (2019) Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis. Chemical Science 10:5, pages 1506-1513.
Crossref
Alireza Karbalayghareh, Ulisses Braga-Neto & Edward R. Dougherty. (2019) Classification of Single-Cell Gene Expression Trajectories from Incomplete and Noisy Data. IEEE/ACM Transactions on Computational Biology and Bioinformatics 16:1, pages 193-207.
Crossref
Aude I. Segaliny, Guideng Li, Lingshun Kong, Ci Ren, Xiaoming Chen, Jessica K. Wang, David Baltimore, Guikai Wu & Weian Zhao. (2018) Functional TCR T cell screening using single-cell droplet microfluidics. Lab on a Chip 18:24, pages 3733-3749.
Crossref
Kerstin Hartmann, Kornelia Schlombs, Mark Laible, Claudia Gürtler, Marcus Schmidt, Ugur Sahin & Hans-Anton Lehr. (2018) Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue. Diagnostic Pathology 13:1.
Crossref
Daniel Andersson, David Svec, Cathrine Pedersen, Jørn Henriksen & Anders Ståhlberg. (2018) Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons. International Journal of Molecular Sciences 19:10, pages 3185.
Crossref
Kohei Suzuki, Tatsuro Murano, Hiromichi Shimizu, Go Ito, Toru Nakata, Satoru Fujii, Fumiaki Ishibashi, Ami Kawamoto, Sho Anzai, Reiko Kuno, Konomi Kuwabara, Junichi Takahashi, Minami Hama, Sayaka Nagata, Yui Hiraguri, Kento Takenaka, Shiro Yui, Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Ohtsuka, Mamoru Watanabe & Ryuichi Okamoto. (2018) Single cell analysis of Crohn’s disease patient-derived small intestinal organoids reveals disease activity-dependent modification of stem cell properties. Journal of Gastroenterology 53:9, pages 1035-1047.
Crossref
Anders Ståhlberg, Amin El-Heliebi, Peter Sedlmayr & Thomas Kroneis. (2018) Unravelling the biological secrets of microchimerism by single-cell analysis. Briefings in Functional Genomics 17:4, pages 255-264.
Crossref
Claudia Gürtler, Mark Laible, Wolfgang Schwabe, Heike Steinhäuser, Xingmin Li, Shujin Liu, Kornelia Schlombs & Ugur Sahin. (2018) Transferring a Quantitative Molecular Diagnostic Test to Multiple Real-Time Quantitative PCR Platforms. The Journal of Molecular Diagnostics 20:4, pages 398-414.
Crossref
Alireza Karbalayghareh, Ulisses Braga-Neto & Edward R. Dougherty. (2018) Intrinsically Bayesian robust classifier for single-cell gene expression trajectories in gene regulatory networks. BMC Systems Biology 12:S3.
Crossref
Claudia Bevilacqua & Bertrand Ducos. (2018) Laser microdissection: A powerful tool for genomics at cell level. Molecular Aspects of Medicine 59, pages 5-27.
Crossref
Anders Ståhlberg & Mikael Kubista. (2018) Technical aspects and recommendations for single-cell qPCR. Molecular Aspects of Medicine 59, pages 28-35.
Crossref
Alireza Karbalayghareh, Ulisses Braga-Neto, Jianping Hua & Edward Russell Dougherty. (2018) Classification of State Trajectories in Gene Regulatory Networks. IEEE/ACM Transactions on Computational Biology and Bioinformatics 15:1, pages 68-82.
Crossref
Johanna Duda, Michael Fauler, Jan Gründemann & Birgit Liss. 2018. Laser Capture Microdissection. Laser Capture Microdissection 335 360 .
Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer & Andreas Weinhaeusel. 2018. DNA Methylation Protocols. DNA Methylation Protocols 407 424 .
Kajsa Ericson-Lindquist, Anna Johansson, Per Levéen, Göran Elmberger, Göran Jönsson, Johan Staaf & Hans Brunnström. (2017) Targeted sequencing may facilitate differential diagnostics of pulmonary tumours: a case series. Diagnostic Pathology 12:1.
Crossref
Nobutaka Takahashi, Anton N. Shuvaev, Ayumu Konno, Yasunori Matsuzaki, Masashi Watanave & Hirokazu Hirai. (2017) Regulatory connection between the expression level of classical protein kinase C and pruning of climbing fibers from cerebellar Purkinje cells. Journal of Neurochemistry 143:6, pages 660-670.
Crossref
Maren Bredemeier, Philippos Edimiris, Pawel Mach, Mikael Kubista, Robert Sjöback, Eva Rohlova, Katarina Kolostova, Siegfried Hauch, Bahriye Aktas, Mitra Tewes, Rainer Kimmig & Sabine Kasimir-Bauer. (2017) Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer. Clinical Chemistry 63:10, pages 1585-1593.
Crossref
Hilde-Marléne Bergman & Ingela Lanekoff. (2017) Profiling and quantifying endogenous molecules in single cells using nano-DESI MS. The Analyst 142:19, pages 3639-3647.
Crossref
Marina Chulkina, Ulugbek Negmadjanov, Ekaterina Lebedeva, Aleksey Pichugin, Dmitriy Mazurov, Ravshan Ataullakhanov & Ekhson Holmuhamedov. (2017) Synthetic peptide TEKKRRETVEREKE derived from ezrin induces differentiation of NIH/3T3 fibroblasts. European Journal of Pharmacology 811, pages 249-259.
Crossref
Ulrika Wilhelmsson, Daniel Andersson, Yolanda de Pablo, Roy Pekny, Anders Ståhlberg, Jan Mulder, Nicholas Mitsios, Tibor Hortobágyi, Milos Pekny & Marcela Pekna. (2017) Injury Leads to the Appearance of Cells with Characteristics of Both Microglia and Astrocytes in Mouse and Human Brain. Cerebral Cortex 27:6, pages 3360-3377.
Crossref
Amin Forootan, Robert Sjöback, Jens Björkman, Björn Sjögreen, Lucas Linz & Mikael Kubista. (2017) Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification 12, pages 1-6.
Crossref
Thomas Kroneis, Emma Jonasson, Daniel Andersson, Soheila Dolatabadi & Anders Ståhlberg. (2017) Global preamplification simplifies targeted mRNA quantification. Scientific Reports 7:1.
Crossref
Alireza Karbalayghareh, Ulisses Braga-Neto & Edward R. Dougherty. (2017) Classification of Gaussian trajectories with missing data in Boolean gene regulatory networks. Classification of Gaussian trajectories with missing data in Boolean gene regulatory networks.
David Dzamba, Lukas Valihrach, Mikael Kubista & Miroslava Anderova. (2016) The correlation between expression profiles measured in single cells and in traditional bulk samples. Scientific Reports 6:1.
Crossref
Yuanlong Song, Miaomiao Zhang, Xiaoqing Tao, Zifen Xu, Liangpin Zhang, Yunjie Zheng, Minjie Zhu & Linlin Gao. (2016) A Single-Cell-Type Real-Time PCR Method Based on a Modified Patch-Pipette Cell Harvesting System. Molecular Biotechnology 58:8-9, pages 558-565.
Crossref
Adrien Molinas, Imad Aoudé, Vanessa Soubeyre, Bassim Tazir, Hervé Cadiou & Xavier Grosmaitre. (2016) Anatomical and molecular consequences of Unilateral Naris Closure on two populations of olfactory sensory neurons expressing defined odorant receptors. Neuroscience Letters 626, pages 42-47.
Crossref
Dicle Yalcin, Zeynep M. Hakguder & Hasan H. Otu. (2016) Bioinformatics approaches to single-cell analysis in developmental biology. Molecular Human Reproduction 22:3, pages 182-192.
Crossref
Sanjin Hosic, Shashi K. Murthy & Abigail N. Koppes. (2015) Microfluidic Sample Preparation for Single Cell Analysis. Analytical Chemistry 88:1, pages 354-380.
Crossref
Radu Boitor, Faris Sinjab, Stephanie Strohbuecker, Virginie Sottile & Ioan Notingher. (2016) Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography. Faraday Discussions 187, pages 199-212.
Crossref
Bo Shuai, Lin Shen, Rui Zhu & Piqi Zhou. (2015) Effect of Qing’e formula on the in vitro differentiation of bone marrow-derived mesenchymal stem cells from proximal femurs of postmenopausal osteoporotic mice. BMC Complementary and Alternative Medicine 15:1.
Crossref
Vlasta Korenková, Justin Scott, Vendula Novosadová, Marie Jindřichová, Lucie Langerová, David Švec, Monika Šídová & Robert Sjöback. (2015) Pre-amplification in the context of high-throughput qPCR gene expression experiment. BMC Molecular Biology 16:1.
Crossref
Kjetil Hodne & Finn-Arne Weltzien. (2015) Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities. International Journal of Molecular Sciences 16:11, pages 26832-26849.
Crossref
Marek Ingr, Jiří Dostál & Taťána Majerová. (2015) Enzymological description of multitemplate PCR—Shrinking amplification bias by optimizing the polymerase–template ratio. Journal of Theoretical Biology 382, pages 178-186.
Crossref
Petr Svoboda. (2015) A toolbox for miRNA analysis. FEBS Letters 589:14, pages 1694-1701.
Crossref
José-Enrique O’Connor, Guadalupe Herrera, Alicia Martínez-Romero, Francisco Sala de Oyanguren, Laura Díaz, Angela Gomes, Susana Balaguer & Robert C. Callaghan. (2014) Systems Biology and immune aging. Immunology Letters 162:1, pages 334-345.
Crossref
José-Enrique O’Connor, Guadalupe Herrera, Alicia Martínez-Romero, Francisco Sala-de Oyanguren, Laura Díaz, Angela Gomes, Susana Balaguer & Robert C. Callaghan. (2014) WITHDRAWN: Systems Biology and Immune Aging. Immunology Letters.
Crossref