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Original Article

Additives and Enzyme Stability

Pages 187-196 | Received 17 Jul 1987, Accepted 06 Oct 1987, Published online: 11 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Roland Brugger, Alexandra Kronenberger, Andrea Bischoff, Denis Hug, Martin Lehmann, Adolphus P.G.M. Van Loon & Markus Wyss. (2001) Thermostability Engineering of Fungal Phytases Using Low-Mr Additives and Chemical Crosslinking. Biocatalysis and Biotransformation 19:5-6, pages 505-516.
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A. M. Azevedo, L. P. Fonseca & D. M. F. Prazeres. (2000) Kinetic and Stability Studies of Penicillin Acylase in Reversed Micelles. Biocatalysis and Biotransformation 17:6, pages 401-415.
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L. Ludikhuyze, S. De Cordt, C. Weemaes, M. Hendrickx & P. Tobback. (1996) >Kinetics for heat and pressure‐temperature inactivation of bacillus subtilis α‐amylase. Food Biotechnology 10:2, pages 105-129.
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S. A. Barker. (1988) Enzyme Behaviour in Concentrated Media. Biocatalysis 2:1, pages 11-18.
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Articles from other publishers (31)

Juan M. Bolivar, John M. Woodley & Roberto Fernandez-Lafuente. (2022) Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization. Chemical Society Reviews 51:15, pages 6251-6290.
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Mozhgan Mohammadi, Behzad Shareghi, Sadegh Farhadian, Lida Momeni & Ali Akbar Saboury. (2022) The interaction of xylitol with carboxypeptidase A: The influence of xylitol on enzyme structure and activity. Journal of Molecular Structure 1250, pages 131877.
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Mozhgan Mohammadi, Behzad Shareghi, Sadegh Farhadian & Ali Akbar Saboury. (2021) The effect of sorbitol on the structure and activity of carboxypeptidase A: Insights from a spectroscopic and computational approach. Journal of Molecular Liquids 330, pages 115710.
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Diego Carballares, Roberto Morellon-Sterling, Xiaomin Xu, Frank Hollmann & Roberto Fernandez-Lafuente. (2021) Immobilization of the Peroxygenase from Agrocybe aegerita. The Effect of the Immobilization pH on the Features of an Ionically Exchanged Dimeric Peroxygenase. Catalysts 11:5, pages 560.
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Sabrina Ait Braham, El Hocine Siar, Sara Arana-Peña, Hossein Bavandi, Diego Carballares, Roberto Morellon-Sterling, Diandra de Andrades, Jakub F. Kornecki & Roberto Fernandez-Lafuente. (2021) Positive effect of glycerol on the stability of immobilized enzymes: Is it a universal fact?. Process Biochemistry 102, pages 108-121.
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Sabrina Ait Braham, El-Hocine Siar, Sara Arana-Peña, Diego Carballares, Roberto Morellon-Sterling, Hossein Bavandi, Diandra de Andrades, Jakub F. Kornecki & Roberto Fernandez-Lafuente. (2021) Effect of Concentrated Salts Solutions on the Stability of Immobilized Enzymes: Influence of Inactivation Conditions and Immobilization Protocol. Molecules 26:4, pages 968.
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Nisha Jangir, Preeti & Santosh Kumar Padhi. (2020) A study on increasing enzymatic stability and activity of Baliospermum montanum hydroxynitrile lyase in biocatalysis. Process Biochemistry 88, pages 78-89.
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Leanne M. Timpson, Ann-Kathrin Liliensiek, Diya Alsafadi, Jennifer Cassidy, Michael A. Sharkey, Susan Liddell, Thorsten Allers & Francesca Paradisi. (2012) A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii. Applied Microbiology and Biotechnology 97:1, pages 195-203.
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David Van Eylen, Indrawati Oey, Marc Hendrickx & Ann Van Loey. (2008) Effects of pressure/temperature treatments on stability and activity of endogenous broccoli (Brassica oleracea L. cv. Italica) myrosinase and on cell permeability. Journal of Food Engineering 89:2, pages 178-186.
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Tim Baks, Anja E.M. Janssen & Remko M. Boom. (2006) The effect of carbohydrates on ?-amylase activity measurements. Enzyme and Microbial Technology 39:1, pages 114-119.
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Gabriel O. SozziL. Carl Greve, Gerry A. ProdyJohn M. Labavitch. (2002) Gibberellic Acid, Synthetic Auxins, and Ethylene Differentially Modulate α- l -Arabinofuranosidase Activities in Antisense 1-Aminocyclopropane-1-Carboxylic Acid Synthase Tomato Pericarp Discs . Plant Physiology 129:3, pages 1330-1340.
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Cleide M. F. Soares, Heizir F. De Castro, M. Helena A. Santana & Gisella M. Zanin. 2002. Biotechnology for Fuels and Chemicals. Biotechnology for Fuels and Chemicals 863 874 .
Kinga László, Anikó Szava & L. Mária Simon. (2001) Stabilization of various α-chymotrypsin forms in aqueous-organic media by additives. Journal of Molecular Catalysis B: Enzymatic 16:3-4, pages 141-146.
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J. Justin Gooding, Paul Erokhin, Dusan Losic, Wenrong Yang, Vanessa Policarpio, Jingquan Liu, Felix M. Ho, Manihar Situmorang, D. Brynn Hibbert & Joe G. Shapter. (2001) Parameters Important in Fabricating Enzyme Electrodes Using Self-Assembled Monolayers of Alkanethiols. Analytical Sciences 17:1, pages 3-9.
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Giovanni Spagna, Riccardo N. Barbagallo, Angela Martino & Pier Giorgio Pifferi. (2000) A simple method for purifying glycosidases: ?-l-rhamnopyranosidase from Aspergillus niger to increase the aroma of Moscato wine. Enzyme and Microbial Technology 27:7, pages 522-530.
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Ana M Azevedo, Luís P Fonseca & Duarte M F Prazeres. (1999) Stability and stabilisation of penicillin acylase. Journal of Chemical Technology & Biotechnology 74:11, pages 1110-1116.
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A.M. Gon?alves, E. Schacht, G. Matthijs, M.R. Aires Barros, J.M.S. Cabral & M.H. Gil. (1999) Stability studies of a recombinant cutinase immobilized to dextran and derivatized silica supports. Enzyme and Microbial Technology 24:1-2, pages 60-66.
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Michael Bauer, Roland Geyer, Matthias Boy, Herfried Griengl & Walter Steiner. (1998) Stability of the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis. Journal of Molecular Catalysis B: Enzymatic 5:1-4, pages 343-347.
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Adil Anwar & Mohammed Saleemuddin. (1998) Alkaline proteases: A review. Bioresource Technology 64:3, pages 175-183.
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Giovanni Spagna, Denis Romagnoli, Martino Angela, Giovanni Bianchi & Pier Giorgio Pifferi. (1998) A simple Method for Purifying Glycosidases: ?-l-arabinofuranosidase and ?-d-glucopyranosidase from Aspergillus niger to Increase the Aroma of Wine. Part I. Enzyme and Microbial Technology 22:5, pages 298-304.
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Federico Cioci & Roberto Lavecchia. (2004) Molecular thermodynamics of heat‐induced protein unfolding in aqueous media. AIChE Journal 43:2, pages 525-534.
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A. P. V. Gonçalves, J. M. S. Cabral & M. R. Aires-Barros. (1996) Immobilization of a recombinant cutinase by entrapment and by covalent binding. Applied Biochemistry and Biotechnology 60:3, pages 217-228.
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Shihong Li, Thomas W. Patapoff, David Overcashier, Chung Hsu, Tue H. Nguyen & Ronald T. Borchardt. (1996) Effects of Reducing Sugars on the Chemical Stability of Human Relaxin in the Lyophilized State. Journal of Pharmaceutical Sciences 85:8, pages 873-877.
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S. De Cordt, L. Ludikhuyze, C. Weemaes, M. Hendrickx, K. Heremans & P. Tobback. 1996. High Pressure Bioscience and Biotechnology, Proceedings of the International Conference on High Pressure Bioscience and Biotechnology. High Pressure Bioscience and Biotechnology, Proceedings of the International Conference on High Pressure Bioscience and Biotechnology 203 208 .
M. J. Pires & J. M. S. Cabral. (1994) Thermal stability of aDesulfovibrio gigas periplasmic hydrogenase in reverse micelles. Applied Biochemistry and Biotechnology 48:3, pages 137-147.
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S. de Cordt, M. Hendrickx, G. Maesmans & P. Tobback. (2004) The influence of polyalcohols and carbohydrates on the thermostability of α‐amylase. Biotechnology and Bioengineering 43:2, pages 107-114.
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Ernst Wehtje, Patrick Adlercreutz & Bo Mattiasson. (2004) Improved activity retention of enzymes deposited on solid supports. Biotechnology and Bioengineering 41:2, pages 171-178.
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S. De Cordt, J. Saraiva, M. Hendrickx, G. Maesmans & P. Tobback. 1993. Stability and Stabilization of Enzymes. Stability and Stabilization of Enzymes 261 268 .
H.M. Pinheiro & J.M.S. Cabral. (1992) Screening of whole-cell immobilization procedures for the Δ1-dehydrogenation of steroids in organic medium. Enzyme and Microbial Technology 14:8, pages 619-624.
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S.R. Weijers & K. Van't Riet. (1992) Enzyme stability in downstream processing part 1: Enzyme inactivation, stability and stabilization. Biotechnology Advances 10:2, pages 237-249.
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H. M. Pinheiro & J. M. S. Cabral. (2004) Effects of solvent molecular toxicity and microenvironment composition on the Δ 1 dehydrogenataon activity of Arthrobacter simplex cells . Biotechnology and Bioengineering 37:2, pages 97-102.
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