Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

Yun Lin Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Humilidad F. Gallardo Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Geoffrey Y. Ku Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Hao Li Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Gregor Manukian Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Teresa S. Rasalan Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Yinyan Xu Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Stephanie L. Terzulli Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Lloyd J. Old Ludwig Institute for Cancer Research, New York Branch

James P. Allison Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; Howard Hughes Medical Institute; Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA

Alan N. Houghton Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute

Jedd D. Wolchok Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; Ludwig Institute for Cancer Research, New York Branch; Howard Hughes Medical Institute; The last two authors contributed equally to this study. Yun Lin, Lloyd J. Old, James P. Allison, Alan N. Houghton, Jedd D. Wolchok and Jianda Yuan designed research. Yun Lin, Humilidad F. Gallardo, Hao Li, Gregor Manukian, Teresa S. Rasalan, Yinyan Xu, Stephanie L. Terzulli and Jianda Yuan performed research. Yun Lin and Jianda Yuan analyzed data. Yun Lin, Geoffrey Y. Ku, Jedd D. Wolchok and Jianda Yuan wrote the paper.Correspondence[email protected]

Jianda Yuan Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; The last two authors contributed equally to this study. Yun Lin, Lloyd J. Old, James P. Allison, Alan N. Houghton, Jedd D. Wolchok and Jianda Yuan designed research. Yun Lin, Humilidad F. Gallardo, Hao Li, Gregor Manukian, Teresa S. Rasalan, Yinyan Xu, Stephanie L. Terzulli and Jianda Yuan performed research. Yun Lin and Jianda Yuan analyzed data. Yun Lin, Geoffrey Y. Ku, Jedd D. Wolchok and Jianda Yuan wrote the paper.

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Zhi-Chun Ding, Chufeng Liu, Yang Cao, Tsadik Habtetsion, Michal Kuczma, Wenhu Pi, Heng Kong, Ercan Cacan, Susanna F. Greer, Yan Cui, Bruce R. Blazar, David H. Munn & Gang Zhou. (2016) IL-7 signaling imparts polyfunctionality and stemness potential to CD4+ T cells. OncoImmunology 5:6.
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J. Joseph Melenhorst. (2009) Optimizing T-cell expansion: have we reached the limit?. Cytotherapy 11:7, pages 813-814.
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Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

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Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

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