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Cytotherapy Volume 11, 2009 - Issue 7
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Research Article

Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

Yun Lin Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Humilidad F. Gallardo Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Geoffrey Y. Ku Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Hao Li Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Gregor Manukian Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Teresa S. Rasalan Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Yinyan Xu Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Stephanie L. Terzulli Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Lloyd J. Old Ludwig Institute for Cancer Research, New York Branch
,
James P. Allison Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; Howard Hughes Medical Institute; Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA
,
Alan N. Houghton Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute
,
Jedd D. Wolchok Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; Ludwig Institute for Cancer Research, New York Branch; Howard Hughes Medical Institute; The last two authors contributed equally to this study. Yun Lin, Lloyd J. Old, James P. Allison, Alan N. Houghton, Jedd D. Wolchok and Jianda Yuan designed research. Yun Lin, Humilidad F. Gallardo, Hao Li, Gregor Manukian, Teresa S. Rasalan, Yinyan Xu, Stephanie L. Terzulli and Jianda Yuan performed research. Yun Lin and Jianda Yuan analyzed data. Yun Lin, Geoffrey Y. Ku, Jedd D. Wolchok and Jianda Yuan wrote the paper.Correspondence[email protected]
&
Jianda Yuan Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute; The last two authors contributed equally to this study. Yun Lin, Lloyd J. Old, James P. Allison, Alan N. Houghton, Jedd D. Wolchok and Jianda Yuan designed research. Yun Lin, Humilidad F. Gallardo, Hao Li, Gregor Manukian, Teresa S. Rasalan, Yinyan Xu, Stephanie L. Terzulli and Jianda Yuan performed research. Yun Lin and Jianda Yuan analyzed data. Yun Lin, Geoffrey Y. Ku, Jedd D. Wolchok and Jianda Yuan wrote the paper.
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Pages 912-922 | Published online: 10 Nov 2009

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