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Cytotherapy Volume 11, 2009 - Issue 7
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Research Article

Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro

Bettina Lindroos University of Tampere and Tampere University Hospital, Regea Institute for Regenerative Medicine, Tampere, FinlandCorrespondence[email protected]
,
Shayne Boucher Life Technologies, GIBCO Cell Culture, Primary and Stem Cell Systems, Frederick, Maryland, USA
,
Lucas Chase Life Technologies, Drug Discovery, Primary and Stem Cell Systems, Madison, Wisconsin, USA
,
Hannu Kuokkanen Department of Plastic Surgery, Tampere University Hospital, Tampere, Finland
,
Heini Huhtala Tampere School of Public Health, University of Tampere, Tampere, Finland
,
Riina Haataja Tampere School of Public Health, University of Tampere, Tampere, Finland
,
Mohan Vemuri Life Technologies, GIBCO Cell Culture, Primary and Stem Cell Systems, Frederick, Maryland, USA
,
Riitta Suuronen University of Tampere and Tampere University Hospital, Regea Institute for Regenerative Medicine, Tampere, Finland; Department of Eye, Ear and Oral Diseases, Tampere University Hospital, Finland; Department of Biomedical Engineering, Tampere University of Technology, Finland
&
Susanna Miettinen University of Tampere and Tampere University Hospital, Regea Institute for Regenerative Medicine, Tampere, Finland
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Pages 958-972 | Published online: 10 Nov 2009

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Alonso Ornelas-González, Mirna González-González & Marco Rito-Palomares. (2021) Microcarrier-based stem cell bioprocessing: GMP-grade culture challenges and future trends for regenerative medicine. Critical Reviews in Biotechnology 41:7, pages 1081-1095.
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Bettina Mannerström, Roman Kornilov, Ahmed G. Abu-Shahba, Iftekhar M. Chowdhury, Snehadri Sinha, Riitta Seppänen-Kaijansinkko & Sippy Kaur. (2019) Epigenetic alterations in mesenchymal stem cells by osteosarcoma-derived extracellular vesicles. Epigenetics 14:4, pages 352-364.
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Roman Kornilov, Maija Puhka, Bettina Mannerström, Hanna Hiidenmaa, Hilkka Peltoniemi, Pia Siljander, Riitta Seppänen-Kaijansinkko & Sippy Kaur. (2018) Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum. Journal of Extracellular Vesicles 7:1.
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Laura Kyllönen, Suvi Haimi, Janne Säkkinen, Hannu Kuokkanen, Bettina Mannerström, George K. B. Sándor & Susanna Miettinen. (2013) Exogenously added BMP-6, BMP-7 and VEGF may not enhance the osteogenic differentiation of human adipose stem cells. Growth Factors 31:5, pages 141-153.
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Sreedhar Thirumala, W. Scott Goebel & Erik J Woods. (2013) Manufacturing and banking of mesenchymal stem cells. Expert Opinion on Biological Therapy 13:5, pages 673-691.
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Parvathy Venugopal, Sudha Balasubramanian, Anish Sen Majumdar & Malancha Ta. (2011) Isolation, characterization, and gene expression analysis of Wharton’s jelly-derived mesenchymal stem cells under xeno-free culture conditions. Stem Cells and Cloning: Advances and Applications 4, pages 39-50.
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Lan Huang, Paul J. Critser, Brenda R. Grimes & Mervin C. Yoder. (2011) Human umbilical cord blood plasma can replace fetal bovine serum for in vitro expansion of functional human endothelial colony-forming cells. Cytotherapy 13:6, pages 712-721.
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Pedro P. Carvalho, Xiying Wu, Gang Yu, Marilyn Dietrich, Isabel R. Dias, Manuela E. Gomes, Rui L. Reis & Jeffrey M. Gimble. (2011) Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. Cytotherapy 13:5, pages 594-597.
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Darryl R Davis & Duncan J Stewart. (2011) Autologous cell therapy for cardiac repair. Expert Opinion on Biological Therapy 11:4, pages 489-508.
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