Regulatory effects of sulfur on oilseed rape (Brassica napus L.) response to selenite

Figures & data

Table 1. Physical and chemical properties of the soil tested.

Potential of hydrogen (pH) (water:soil = 1:2.5)	Organic matter (OM) (g kg^−1)	Available nitrogen (N) (mg kg^−1)	Olsen-phosphorus (P) (mg kg^−1)	Available potassium (K) (mg kg^−1)	Available sulfur (S) (mg kg^−1)	Total selenium (Se) (mg kg^−1)
7.83	18.42	54.92	4.61	113.00	17.82	0.28

Table 2. Biomass of oilseed rape (Brassica napus L.) under different sulfur (S) and selenium (Se) treatments in soil culture. Values are means ± standard error (SE; dry matter basis).

S added	Se added	Biomass of different parts (g plant^−1)
(mg kg^−1)	(mg kg^−1)	Seedling stage	Maturity stage
		Shoot	Root	Stem	Pod	Seed
0	0	0.62 ± 0.01a	2.75 ± 0.05bc	11.71 ± 0.55ab	6.75 ± 0.27ab	6.80 ± 0.24ab
	5	0.64 ± 0.03a	2.84 ± 0.07ab	11.86 ± 0.53ab	6.87 ± 0.21ab	6.93 ± 0.22ab
	15	0.51 ± 0.03b	2.45 ± 0.09c	11.20 ± 0.53b	6.60 ± 0.22b	6.18 ± 0.08b
150	0	0.64 ± 0.03a	3.06 ± 0.13ab	13.01 ± 0.50a	7.37 ± 0.26a	7.38 ± 0.21a
	5	0.67 ± 0.03a	3.12 ± 0.15a	13.15 ± 0.57a	7.50 ± 0.24a	7.49 ± 0.28a
	15	0.59 ± 0.02ab	2.93 ± 0.12ab	12.85 ± 0.40a	7.15 ± 0.18ab	7.11 ± 0.23a
300	0	0.58 ± 0.02ab	3.09 ± 0.06a	13.25 ± 0.42a	7.11 ± 0.19ab	7.23 ± 0.28a
	5	0.62 ± 0.03a	3.14 ± 0.10a	13.32 ± 0.45a	7.24 ± 0.22ab	7.33 ± 0.34a
	15	0.53 ± 0.04b	3.05 ± 0.13ab	13.18 ± 0.48a	7.13 ± 0.24ab	6.89 ± 0.19ab

Different lowercase letters indicate significant differences at p < 0.05.

Figure 1. pH in soil at the mature stage with different concentrations of sulfur (S) and selenium (Se). Bars indicate standard error (n = 4). Different lowercase letters indicate significant differences at p < 0.05.

Figure 2. Selenium (Se) concentration in the shoots of Brassica napus L. grown at the seedling stage with different concentrations of sulfur (S) and Se. Bars indicate standard error (n = 4). Different lowercase letters indicate significant differences at p < 0.05.

Figure 3. Selenium (Se) concentrations in various parts of Brassica napus L. grown at the mature stage with different concentrations of sulfur (S) and Se. Bars indicate standard error (n = 4). Different lowercase letters indicate significant differences at p < 0.05.

Table 3. Selenium (Se) accumulation in various parts of Brassica napus L. grown at the maturity stage with different concentrations of sulfur (S) and Se. The values are means ± standard error (SE; n = 4).

S added	Se added	Se accumulation in different parts (g plant^−1)				Total Se accumulation
(mg kg^−1)	(mg kg^−1)	Root	Stem	Pod	Seed	(g plant^−1)
0	0	0.65 ± 0.05f	1.26 ± 0.17f	1.29 ± 0.16f	0.79 ± 0.08f	3.99 ± 0.05f
	5	22.40 ± 0.81d	43.49 ± 2.77d	51.56 ± 3.43d	34.09 ± 1.94d	151.54 ± 0.05d
	15	60.49 ± 3.49a	123.98 ± 6.40a	138.35 ± 7.58a	93.25 ± 5.34a	416.07 ± 0.05a
150	0	0.49 ± 0.01f	1.11 ± 0.14f	1.02 ± 0.06f	0.69 ± 0.09f	3.31 ± 0.05f
	5	16.23 ± 0.90e	29.10 ± 2.52e	31.53 ± 2.39e	22.46 ± 0.78e	99.32 ± 0.05e
	15	54.59 ± 3.82b	110.79 ± 6.43b	96.50 ± 4.72b	75.75 ± 2.09b	337.63 ± 0.05b
300	0	0.48 ± 0.03f	1.12 ± 0.16f	1.01 ± 0.10f	0.74 ± 0.05f	3.35 ± 0.05f
	5	14.73 ± 1.23e	28.24 ± 1.50e	28.46 ± 2.91e	20.32 ± 1.75e	91.75 ± 0.05e
	15	34.78 ± 1.90c	57.82 ± 4.06c	73.12 ± 3.68c	48.07 ± 3.91c	213.79 ± 0.05c

Different lowercase letters indicate significant differences at p < 0.05.

Figure 4. Selenium (Se) distribution ratio in various parts of Brassica napus L. grown at the mature stage with different concentrations of sulfur (S) and Se. Values are means of four replicates.