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Research Article

Differential changes in expression of inflammatory mRNA and protein after oleic acid-induced acute lung injury

, , , , , , , & ORCID Icon show all
Pages 96-105 | Received 28 Apr 2023, Accepted 03 Apr 2024, Published online: 16 Apr 2024

Figures & data

Table 1. Primer sequences.

Figure 1. Average response of oxygenation index, Vd/Vt ratio, and compliance comparing baseline with ARDS.

Figure 1. Average response of oxygenation index, Vd/Vt ratio, and compliance comparing baseline with ARDS.

Table 2. Laboratory and physiology data.

Figure 2. Fold changes (ARDS relative to baseline) for cytokines measured using RT-qPCR. During ARDS, INF-γ and IL-6 decreased to 59% and 44% of its baseline value, respectively. TNF-α, IL-17, and IL-10 had non-significant fold changes.

Figure 2. Fold changes (ARDS relative to baseline) for cytokines measured using RT-qPCR. During ARDS, INF-γ and IL-6 decreased to 59% and 44% of its baseline value, respectively. TNF-α, IL-17, and IL-10 had non-significant fold changes.

Figure 3. No significance between time to ARDS and cytokine levels. Relative concentrations of mRNA of cytokines had no significant correlation with time it induces ARDS.

Figure 3. No significance between time to ARDS and cytokine levels. Relative concentrations of mRNA of cytokines had no significant correlation with time it induces ARDS.

Figure 4. Fold changes (ARDS relative to baseline) for plasma cytokines measured by ELISA. The significance of change is represented by p values <0.05.

Figure 4. Fold changes (ARDS relative to baseline) for plasma cytokines measured by ELISA. The significance of change is represented by p values <0.05.

Figure 5. Correlation between IL-1β and time needed to reach ARDS. Significant correlation was found between IL-1β levels at ARDS and the time it took the animal to reach ARDS.

Figure 5. Correlation between IL-1β and time needed to reach ARDS. Significant correlation was found between IL-1β levels at ARDS and the time it took the animal to reach ARDS.