Effects of deer bone extract on the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced osteoarthritic rats

Figures & data

Table 1. Contents of components in deer bone extract.

Item	Contents (%)
Crude protein (collagen)	80.94 (30.81)
Crude lipid	12.47
Ash	3.52
Moisture	3.07
Chondroitin sulfate	2.29
Ganglioside	0.11

Table 2. Body and organ weights of the MIA-induced OA rats treated with deer bone extract for 50 days.

Weight (g)	Groups
	SC	NC	PC	LDB	HDB
Body	334.1 ± 16.4^a	302.5 ± 11.4^b	317.3 ± 18.2^ab	309.4 ± 32.1^ab	320.8 ± 20.1^ab
Liver	8.7 ± 0.9^NS	7.8 ± 0.5	8.3 ± 0.5	7.7 ± 1.6	8.7 ± 0.6
Heart	1.0 ± 0.1^NS	0.9 ± 0.1	0.9 ± 0.1	0.9 ± 0.1	0.9 ± 0.1
Spleen	0.6 ± 0.1^NS	0.6 ± 0.1	0.6 ± 0.1	0.6 ± 0.1	0.6 ± 0.1
Kidney	2.1 ± 0.2^NS	2.0 ± 0.2	2.0 ± 0.2	1.9 ± 0.3	1.8 ± 0.5

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract). NS; not significant.

Fig. 1. IL-1β (A) and IL-6 (B) levels in serum of the MIA-induced OA rats treated with deer bone extract for 50 days.

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract).

Fig. 2. TNF-α level in serum of the MIA-induced OA rats treated with deer bone extract for 50 days.

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract).

Fig. 3. COL2 mRNA expression in articular cartilage of the MIA-induced OA rats treated with deer bone extract for 50 days.

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract).

Fig. 4. COL2 mRNA expression in articular cartilage of the MIA-induced OA rats treated with deer bone extract for 50 days.

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract).

Fig. 5. TIMPs mRNA expression in articular cartilage of the MIA-induced OA rats treated with deer bone extract for 50 days.

Note: Values are mean ± SD. Differences between groups (10 rats/group) were analyzed using one-way ANOVA by Tukey’s multiple range tests. Means with different superscript letters are significantly different at p < 0.05. Each group was assigned as follows: SC (PBS injection + non-treatment); NC (MIA injection + non-treatment); PC (MIA injection + 125 mg/kg glucosamine sulfate + 125 mg/kg chondroitin sulfate); LDB (MIA injection + 250 mg/kg deer bone extract); HDB (MIA injection + 500 mg/kg deer bone extract).