Advanced search
Publication Cover
Food and Agricultural Immunology Volume 30, 2019 - Issue 1
Submit an article Journal homepage
1,606
Views
3
CrossRef citations to date
0
Altmetric
Articles

Extract of curry powder and its components protect against diesel exhaust particle-induced inflammatory responses in human airway epithelial cells

Akiko HondaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanCorrespondence[email protected]
View further author information
,
Sho ItoEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Michitaka TanakaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Takahiro SawaharaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Tomohiro HayashiEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Wataru FukushimaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Gaku KitamuraEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Hitomi KudoEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Pratiti Home ChowdhuryEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Hitoshi OkanoEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Toshinori OnishiEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Yusuke KawaryuEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Makoto HigashiharaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
,
Hideki NakayamaResearch & Development Institute, House Foods Corporation, Yotsukaido, JapanView further author information
,
Kayo UedaEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
&
Hirohisa TakanoEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, JapanView further author information
 show all
Pages 1212-1224 | Received 27 Aug 2019, Accepted 11 Oct 2019, Published online: 31 Oct 2019

Figures & data

Figure 1. Effects of curry powder, clove, and turmeric extracts on DEP-induced IL-6 release in human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. IL-6 release was measured by ELISA. Data are represented as mean ± SE of three individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP; §§ p < 0.01, vs each other.

Figure 1. Effects of curry powder, clove, and turmeric extracts on DEP-induced IL-6 release in human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. IL-6 release was measured by ELISA. Data are represented as mean ± SE of three individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP; §§ p < 0.01, vs each other.

Figure 2. Effects of curry powder, clove, and turmeric extracts on DEP-induced viability of human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. Viability was measured by WST-1 assay. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP.

Figure 2. Effects of curry powder, clove, and turmeric extracts on DEP-induced viability of human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. Viability was measured by WST-1 assay. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP.

Figure 3. Effects of curry powder, clove, and turmeric extracts on DEP-induced extracellular (A) and intracellular (B) ROS. The extracellular levels of ROS were measured after exposure to DEP and each extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Cells were exposed to DEP and each extract, and intracellular levels of ROS after 6 h exposure were also measured. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP; §§ p < 0.01, vs each other.

Figure 3. Effects of curry powder, clove, and turmeric extracts on DEP-induced extracellular (A) and intracellular (B) ROS. The extracellular levels of ROS were measured after exposure to DEP and each extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Cells were exposed to DEP and each extract, and intracellular levels of ROS after 6 h exposure were also measured. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP; §§ p < 0.01, vs each other.

Figure 4. Effects of curry powder extract on DEP-induced IL-6 release (A) and extracellular ROS (B) in the absence of clove and turmeric. Cells were exposed to the indicated concentrations of DEP and extract for 24 h. IL-6 release was measured by ELISA. The extracellular levels of ROS were measured after DEP and extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Data are represented as mean ± SE of three or four individual cultures. **p < 0.01, *p < 0.05, versus vehicle; ##p < 0.01, #p < 0.05, versus DEP.

Figure 4. Effects of curry powder extract on DEP-induced IL-6 release (A) and extracellular ROS (B) in the absence of clove and turmeric. Cells were exposed to the indicated concentrations of DEP and extract for 24 h. IL-6 release was measured by ELISA. The extracellular levels of ROS were measured after DEP and extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Data are represented as mean ± SE of three or four individual cultures. **p < 0.01, *p < 0.05, versus vehicle; ##p < 0.01, #p < 0.05, versus DEP.

Figure 5. Effects of various extracts of curry powder components on DEP-induced IL-6 release in human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. IL-6 release was measured by ELISA. Data are represented as mean ± SE of three individual cultures. **p < 0.01, *p < 0.05, versus vehicle; ##p < 0.01, #p < 0.05, versus DEP.

Figure 5. Effects of various extracts of curry powder components on DEP-induced IL-6 release in human airway epithelial cells. Cells were exposed to the indicated concentrations of DEP and each extract for 24 h. IL-6 release was measured by ELISA. Data are represented as mean ± SE of three individual cultures. **p < 0.01, *p < 0.05, versus vehicle; ##p < 0.01, #p < 0.05, versus DEP.

Figure 6. Effects of coriander or cinnamon extract on DEP-induced extracellular ROS. The extracellular level of ROS was measured after DEP and each extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP.

Figure 6. Effects of coriander or cinnamon extract on DEP-induced extracellular ROS. The extracellular level of ROS was measured after DEP and each extract reacted with CM-H2DCFDA fluorescent probe for 3 h. Data are represented as mean ± SE of four individual cultures. **p < 0.01, versus vehicle; ##p < 0.01, versus DEP.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.