Figures & data
Figure 1 Reduction of progesterone to 20α-hydroxy-4-pregnen-3-one by 20α-HSD.
Figure 2 Inhibitory effects of diesel exhaust components on 20α-HSD activity in cytosolic fractions from the liver, kidney and lung of male mice. Progesterone at a concentration of 100 μM was used as the substrate. The concentration of diesel exhaust components was 10 μM. Each bar represents the mean ± SD of three to seven experiments.
Figure 3 Inhibitory effects of flavonoids on 20α-HSD activity in cytosolic fractions from the liver and kidney of male mice. Progesterone at a concentration of 100 μM was used as the substrate. The concentrations of flavonoids were 20 and 50 μM. Each bar represents the mean ± SD of three experiments. Data in cytosolic fraction from the liver were cited from reference [Citation15].
![Figure 3 Inhibitory effects of flavonoids on 20α-HSD activity in cytosolic fractions from the liver and kidney of male mice. Progesterone at a concentration of 100 μM was used as the substrate. The concentrations of flavonoids were 20 and 50 μM. Each bar represents the mean ± SD of three experiments. Data in cytosolic fraction from the liver were cited from reference [Citation15].](/cms/asset/caa35fdb-3160-447e-bcca-411a871110a1/ienz_a_216140_f0003_b.gif)
Figure 4 pH dependence of 20α-HSD activity in cytosolic fractions from the liver and kidney of male mice. Progesterone at a concentration of 100 μM was used as the substrate. Each point represents the mean ± SD of three experiments.
Figure 5 Inhibitory effects of specific inhibitors on 20α-HSD activity in cytosolic fractions from the liver and kidney of male mice. Progesterone at a concentration of 100 μM was used as the substrate. The concentration of specific inhibitors (SBP, 2-CBA and SSA) was 100 μM. SBP is a potent inhibitor of AKR1C20. 2-CBA and SSA are substrate inhibitors for AKR7A1 and AKR7A5. Each bar represents the mean ± SD of three experiments.
