In-vitro evaluation of selected chalcones for antioxidant activity

Figures & data

Figure 1 Structures of the studied SCs.

Table I.  DPPH radical scavenging activity (% DPPH), reducing ability (% RA) and OH radical scavenging activity (% OH) of the selected SCs.

SCs	% DPPH	% RA	% OH
C1	17.32 ( ± 0.25)	13.46 ( ± 0.71)	––NR––-
C2	41.97 ( ± 0.54)	37.91 ( ± 0.29)	9.12 ( ± 0.77)
C3	39.35 ( ± 0.92)	47.28 ( ± 0.61)	10.12 ( ± 0.38)
C4	43.67 ( ± 0.83)	51.23 ( ± 0.48)	14.23 ( ± 0.23)
C5	17.58 ( ± 0.59)	16.27 ( ± 0.19)	–––NR––
C6	16.98 ( ± 0.26)	14.73 ( ± 0.72)	7.12 ( ± 0.95)
C7	14.27 ( ± 1.2)	12.18 ( ± 0.52)	–––NR––
C8	32.17 ( ± 0.71)	26.86 ( ± 0.38)	11.23 ( ± 0.24)
C9	27.39 ( ± 0.45)	22.98 ( ± 0.92)	8.37 ( ± 0.56)
AA	54.16 ( ± 0.28)	––-ND–––	––ND–––
COU	––ND–––	––ND––	4.6 ( ± 0.61)
GLU	67.78 ( ± 0.19)	62.15 ( ± 0.22)	––ND––

Results summarized here are the mean ± S.D. of n = 3. AA-ascorbic acid, COU-coumarin, GLU-glutathione (reduced), NR-no reaction under experimental conditions, ND-not determined. The final concentration of SCs after dilution in DPPH, RA, and OH reaction mixtures was 0.5, 0.09 and 0.03 mM, respectively.

Table II.  Effect of SCs on PPO activity (% PPO) and on formation of hySdroperoxides (% OOH).

SCs	PPO %	OOH %
C1	39.43 ( ± 0.42)	70.65 ( ± 0.12)
C2	34.57 ( ± 0.81)	35.65 ( ± 0.91)
C3	27.87 ( ± 0.47)	45.27 ( ± 0.53)
C4	18.96 ( ± 1.1)	30.21 ( ± 0.31)
C5	22.68 ( ± 0.53)	36.52 ( ± 0.72)
C6	––NR–––	33.91 ( ± 0.41)
C7	26.56 ( ± 0.85)	18.69 ( ± 0.97)
C8	––NR––	32.98 ( ± 0.62)
C9	24.86 ( ± 0.35)	41.95 ( ± 0.49)
ASA	––ND––	68.34 ( ± 0.66)
L-CYS	87.94 ( ± 0.39)	––ND––

Results shown are the mean ± S.D. of n = 3. ASA-acetyl salicylic acid, L-CYS- L-Cysteine, NR-no reaction under experimental conditions, ND-not determined. The final concentration of SCs after dilution in the PPO and OOH reaction mixtures was 0.11 and 0.09 mM, respectively.