Benzo(a)pyrene-induced genotoxicity: Attenuation by farnesol in a mouse model

Figures & data

Table I.  Effect of pretreatment of Farnesol on B(a)P induced toxicity regarding glutathione content, glutathione S-transferase and Quinone reductase in mouse liver.

Treatment Regimen	Reduced glutathione (nmol GSH / g tissue)	Glutathione-S-transferase (nmol CDNB conjugate formed /min / mg protein)	Quinone reductase (nmoles dichloroindophenol reduced/min/mg protein)
Corn oil treated control	0.586 ± 0.06	29.7 ± 0.09	173.66 ± 1.7
B(a)p alone	0.291 ± 0.009^#	4.5 ± 0.29^#	101.22 ± 1.7^#
B(a)p + FL (D1)	0.326 ± 0.01^*	13.8 ± 4.17^*	127.31 ± 1.5*
B(a)p + FL (D2)	0.368 ± 0.012^*	15.8 ± 0.74^*	139.01 ± 6.4*
Only FL (D2)	0.593 ± 0.005	31.9 ± 7.27	210.7 ± 1.81

Results represent mean ± S.E of five animals\group. Results significantly different from corn oil treated group (#P < 0.001). Results significantly different from B(a)P treated group (*P < 0.001). FL = Farnesol, D1 = 1%kg^{− 1} b.wt. and D2 = 2%kg^{− 1}b.wt.

Figure 1.  Effect of pretreatment of Farnesol on B(a)P induced DNA damage in mouse.

Figure 2.  Effect of pretreatment of Farnesol on B(a)P induced cytochrome P450 enzyme in mouse liver.

Figure 3.  Effect of pretreatment of Farnesol on B(a)P induced DNA adduct formation in mouse liver.

Figure 4.  Effect of pretreatment of Farnesol on B(a)P induced aryl hydrocarbon hydrolase in mouse liver.

Figure 5.  Agarose gel electrophoresis of DNA fragments in mouse liver. DNA was isolated and assessed by agarose gel electrophoresis containing ethidiumbromide. 1-control; 2- FLD1 + B(a)P; 3-FLD2 + B(a)P; 4-onlyFLD2; 5-onlyB(a)P.