Norditerpenoid alkaloids from the roots of Aconitum heterophyllum Wall with antibacterial activity

Figures & data

Table I.  ¹³C–NMR data of compounds 1 and 2 (CDCl₃).

	6-dehydroacetylsepaconitine (1)		13-hydroxylappaconitine (2)
C. No	δC	Multiplicity	δC	Multiplicity
1	77.7	CH	84.8	CH
2	26.5	CH2	26.7	CH2
3	31.6	CH2	33.1	CH2
4	84.6	C	84.6	C
5	44.4	CH	47.9	C
6	206	C	27.2	CH2
7	46.8	CH	47.8	CH
8	79.5	C	68.4	C
9	79.6	C	79.2	C)
10	78.9	C	43.8	CH
11	55.7	C	53.1	C
12	37.4	CH2	35	CH2
13	34.5	CH	78.1	CH
14	87.7	CH	87.4	CH
15	44.7	CH2	32.4	CH2
16	82.7	CH	82.2	CH
17	61.5	CH	58	CH
19	55.5	CH2	55.8	CH2
1′	115.7	C	115.7	C
2′	141.6	C	140.1	C
3′	120.2	CH	120.3	CH
4′	134.4	CH	134.4	CH
5′	122.3	CH	122.3	CH
6′	130.9	CH	131	CH
	48.9	CH2	49	CH2
	13.4	CH3	13.5	CH3
C = O	167	C	159.7	C
	169.3	C	168	C
	21.4	CH3	25.2	CH3

Table II.  Table-2 Antibacterial activities of compounds 1-5. Relative to the standard drug Imipenem. For details, see Experimental.

Compound	EC	BS	SF	SA	PA	ST
Imipenem	30	33	27	33	24	25
1	–	15	12	24	–	15
2	10	15	10	–	13	17
3	11	16	15	15	17	17
4	–	12	–	15	16	18
5	–	15	15	–	17	17

Inhibition zones are given in mm. Abbreviations: EC, Escherichia coli; BS, Bacillus subtilis; SF, Shigella flexneri; SA, Staphylococcus aureus; PA, Pseudomonas aeruginosa; ST, Salmonella typhi.

Figure 1.  Selective HMBC interactions in compound 1.

Figure 2.  Selective HMBC interactions in compound 2.