A comparative study on the effect of homobrassinolide and gibberellic acid on lipid peroxidation and antioxidant status in normal and diabetic rats

Figures & data

Table 1.  Effect of homobrassinolide (50ug) on lipid peroxidation and antioxidant defense in erythocytes and tissues of normal and diabetic rats.

Tissue	Studies	Parameters	Normal rat	HB-normal rat	Diabetic rat	HB-diabetic rat
Erythocyte	LP	MDA	1.5 ± 0.1	1.1 ± 0.1	2.8 ± 0.2	1.6 ± 0.1**
	AD	4-HNE	1.7 ± 0.2	1.5 ± 0.1**	3.4 ± 0.2	2.1 ± 0.2***
Brain	LP	CAT	190 ± 8	210 ± 12	107 ± 11	125 ± 10
		GSH	80 ± 9	90 ± 15	60 ± 4	83 ± 7
		MDA	56 ± 7	50 ± 7	95 ± 9	74 ± 11
	AD	4-HNE	42 ± 4	40 ± 3	63 ± 4	42 ± 2**
		CAT	30 ± 4	35 ± 2	21 ± 1	27 ± 2
		GSH	80 ± 7	90 ± 6	60 ± 4	75 ± 4***
Liver	LP	MDA	67 ± 5	44 ± 4**	90 ± 7	63 ± 4***
		4-HNE	40 ± 2	35 ± 7	72 ± 5	42 ± 3***
	AD	CAT	15 ± 2	19 ± 3	11 ± 2	15 ± 2
		GSH	86 ± 5	95 ± 5	60 ± 4	80 ± 5**
Kidney	LP	MDA	49 ± 4	33 ± 4*	76 ± 7	51 ± 4*
		4-HNE	46 ± 3	33 ± 2**	70 ± 4	52 ± 4*
	AD	CAT	26 ± 2	28 ± 4	18 ± 3	27 ± 3*
		GSH	62 ± 2	72 ± 3*	41 ± 3	59 ± 4*
Heart	LP	MDA	31 ± 4	27 ± 3	53 ± 4	29 ± 3***
		4-HNE	28 ± 2	24 ± 2	68 ± 6	49 ± 4**
	AD	CAT	20 ± 3	27 ± 2	11 ± 2	16 ± 4
		GSH	60 ± 5	68 ± 5	46 ± 2	51 ± 4
Testis	LP	MDA	26 ± 3	18 ± 2*	35 ± 3	29 ± 3**
		4-HNE	32 ± 2	26 ± 2	63 ± 4	48 ± 2*
	AD	CAT	14 ± 3	16 ± 2	11 ± 1	13 ± 2
		GSH	45 ± 5	49 ± 5	36 ± 1	42 ± 4

MDA and 4-HNE expressed nmol/mL (erythocytes) and nmol/g (tissue). Catalase activity U/mL (erythocytes) and U/g (tissue). GSH content mg/g. p<0.05 *, p<0.01**, p<0.001***. LP: Lipid peroxidation, AD: Antioxidant defense

Table 2.  Effect of giibberellic acid (50μg) on lipid peroxidation and antioxidant defense in erythocytes and tissues of normal and diabetic rats.

Tissues	Studies	Parameters	Normal rat	GBA-normal rat	Diabetic rats	GBA-diabetic rat
Erythocyte	LP	MDA	1.5 ± 0.1	1.7 ± 0.1	2.8 ± 0.1	3.2 ± 0.3**
		4-HNE	1.7 ± 0.2	2 ± 0.1	3.4 ± 0.1	3.7 ± 0.2
	AD	CAT	190 ± 8	169 ± 7	100 ± 11	85 ± 9
		GSH	80 ± 9	68 ± 5*	70 ± 4	63 ± 7
Brain	LP	MDA	56 ± 7	68 ± 6*	90 ± 9	124 ± 5*
		4-HNE	42 ± 4	53 ± 4**	61 ± 4	80 ± 4**
	AD	CAT	30 ± 4	26 ± 3*	20 ± 1	12 ± 2**
		GSH	80 ± 7	68 ± 6	58 ± 4	53 ± 4
Liver	LP	MDA	67 ± 5	89 ± 3	87 ± 7	99 ± 5
		4-HNE	40 ± 2	52 ± 5**	72 ± 5	84 ± 7
	AD	CAT	15 ± 2	13 ± 2	11 ± 1	7 ± 1*
		GSH	86 ± 5	65 ± 6*	58 ± 4	43 ± 3**
Kidney	LP	MDA	49 ± 4	55 ± 4	70 ± 7	90 ± 6
		4-HNE	46 ± 3	66 ± 3**	71 ± 4	102 ± 7**
	AD	CAT	26 ± 2	16 ± 1	20 ± 3	8 ± 1*
		GSH	62 ± 2	45 ± 3*	41 ± 2	28 ± 4
Heart	LP	MDA	31 ± 4	48 ± 2*	53 ± 4	89 ± 8**
		4-HNE	28 ± 2	35 ± 4	63 ± 6	75 ± 5
	AD	CAT	20 ± 3	11 ± 2*	12 ± 2	7 ± 1
		GSH	60 ± 5	39 ± 4*	46 ± 1	31 ± 4**
Testis	LP	MDA	26 ± 3	34 ± 4	43 ± 4	76 ± 6***
		4-HNE	32 ± 2	54 ± 4**	63 ± 4	74 ± 7
	AD	CAT	14 ± 3	10 ± 2	13 ± 1	8 ± 1
		GSH	45 ± 5	39 ± 4	36 ± 2	30 ± 4

MDA and 4-HNE expressed nmol/mL (erythocytes) and nmol/g (tissue). Catalase activity U/mL (erythocytes) and U/g (tissue). GSH content mg/g. p<0.05 *, p<0.01**, p<0.001***. LP: Lipid peroxidation, AD: Antioxidant defense