Screening and evaluation of antioxidant activity of some amido-carbonyl oxime derivatives and their radical scavenging activities

Figures & data

Figure 1.  The structure of the oximes and its derivatives.

Figure 2.  Structure of the amido-carbonyl oximes used in this work.

Table 1.  Total antioxidant activities (%) of the oximes, BHA, BHT and trolox at different concentration (5 and 50 μM) in the linoleic acid emulsion system by the FTC method.

Compounds	R	Total antioxidant activity,%
		5 μM	50 μM
1	H	61.13±1.11	55.55±2.42
2	Cl	72.69±2.70	45.17±1.21
3	CH3	64.87±1.15	66.77±3.11
4	OCH3	53.82±4.62	69.29±1.80
5	3,4-diCl	74.42±3.17	78.61±2.73
6	3,4- diCH3	71.07±4.11	72.64±3.75
7	3-Cl-4-CH3	50.75±1.20	68.62±1.94
8	3Cl-4-OCH3	56.56±1.16	82.06±1.43
9	Naphthyl	76.71±3.03	75.71±1.94
10	Dioxim	79.09±4.72	84.73±0.82
11	Trolox	67.25±3.74	79.50±2.41
12	BHA	59.47±1.41	72.75±1.66
13	BHA	71.62±1.31	77.72±2.82

Table 2.  Reducing power of different concentrations (5 and 50 μM) of oximes, BHA, BHT and trolox.

Compounds	R	Reducing power,Abs
		5 μM	50 μM
1	H	0.16±0.007	0.17±0.013
2	Cl	0.153±0.011	0.185±0.008
3	CH3	0.132±0.018	0.187±0.004
4	OCH3	0.132±0.009	0.205±0.007
5	3,4-diCl	0.157±0.007	0.169±0.004
6	3,4- diCH3	0.158±0.008	0.233±0.015
7	3-Cl-4-CH3	0.146±0.017	0.176±0.017
8	3Cl-4-OCH3	0.101±0.003	0.194±0.005
9	Naphthyl	0.144±0.017	0.204±0.009
10	Dioxim	0.139±0.008	0.181±0.010
11	Trolox	0.146±0.011	0.167±0.005
12	BHA	0.152±0.006	0.227±0.008
13	BHA	0.141±0.007	0.215±0.011

Table 3.  DPPH^· free radical scavenging activity (IC₅₀: μg/mL) of oximes. BHA. BHT and Trolox.

Compounds	R	DPPH^· free radical scavenging activity, IC50
1	H	170.47±5.14
2	Cl	144.21±8.20
3	CH3	104.09±4.26
4	OCH3	44.43±2.54
5	3,4-diCl	48.16±4.07
6	3,4- diCH3	43.50±3.17
7	3-Cl-4-CH3	116.67±6.82
8	3Cl-4-OCH3	62.68±8.19
9	Naphthyl	46.53±4.08
10	Dioxim	42.91±2.27
11	Trolox	59.50±7.41
12	BHA	57.18±3.52
13	BHA	39.11±5.06

Table 4.  Ferrous ion (Fe²⁺) chelating activity (%) of oximes and the standard antioxidants BHA. BHT and trolox at different concentrations (5 and 50 μM).

Compounds	R	Reducing power. Abs
		5 μM	50 μM
1	H	52.03±1.18	61.08±2.04
2	Cl	70.58±2.10	81.90±1.29
3	CH3	69.23±1.88	80.76±5.10
4	OCH3	72.85±2.60	80.99±1.30
5	3,4-diCl	72.39±3.41	82.80±2.27
6	3,4- diCH3	70.58±5.10	81.67±3.78
7	3-Cl-4-CH3	72.85±1.28	60.40±1.10
8	3Cl-4-OCH3	70.81±4.1	82.12±1.91
9	Naphthyl	70.13±3.47	82.12±1.97
10	Dioxim	66.74±2.44	81.22±0.28
11	Trolox	74.66±1.74	73.30±2.84
12	BHA	72.85±1.84	73.52±2.86
13	BHA	37.03±4.53	54.16±1.78

Table 5.  Superoxide anion scavenging activity (%) of oximes and the standard antioxidants BHA, BHT and trolox at different concentrations (5 and 50 μM).

Compounds	R	Superoxide anion scavenging activity, %
		5 μM	50 μM
1	R: H	52.03±2.45	61.08±5.21
2	R: Cl	70.58±3.47	81.90±8.40
3	R:CH3	69.23±1.47	80.76±4.81
4	R:OCH3	72.85±2.51	80.99±1.77
5	R: 3,4-diCl	72.39±3.47	82.80±2.82
6	R: 3,4- diCH3	70.58±5.44	81.67±3.41
7	R: 3-Cl-4-CH3	72.85±2.88	60.40±5.47
8	R: 3Cl-4-OCH3	70.81±2.41	82.12±3.97
9	R: Naphthyl	70.13±2.91	82.12±3.64
10	R: Dioxim	66.74±2.45	81.22±2.55
11	Trolox	74.66±2.87	73.30±7.28
12	BHA	72.85±4.15	73.52±5.61
13	BHA	68.55±3.11	76.69±3.75

Table 6.  Hydrogen peroxide scavenging activity (%) of oximes and the standard antioxidants BHA, BHT and trolox at different concentrations (5 and 50 μM).

Compounds	R	Hydrogen peroxide scavenging activity, %
		5 μM	50 μM
1	H	13.33±2.89	7.76±3.41
2	Cl	37.04±5.21	37.29±2.47
3	CH3	43.29±4.87	42.74±2.16
4	OCH3	42.39±2.08	35.96±1.99
5	3,4-diCl	24.29±2.54	29.89±3.91
6	3,4- diCH3	26.43±2.08	34.88±2.41
7	3-Cl-4-CH3	44.63±2.55	36.73±1.34
8	3Cl-4-OCH3	2.56±2.08	3.89±3.74
9	Naphthyl	19.71±2.54	40.79±3.11
10	Dioxim	30.67±2.74	41.03±2.73
11	Trolox	30.67±5.07	33.67±1.57
12	BHA	28.04±2.41	34.75±2.66
13	BHA	27.76±2.21	31.53±1.31