Role of PTPN22 and VDR gene polymorphisms in susceptibility to rheumatoid arthritis: a study from central India

Figures & data

Figure 1 Gel 1 representative image of isolated DNA of case and control.

Notes: L1, Eco RI/Hind III double digested lambda DNA; 2–6 contain isolated DNA; and 7 is negative control.

Table 1 Association of individual protein tyrosine phosphatase, non-receptor type 22 (PTPN22) genotypes, alleles and their carriage rates

PTPN22 genotype	Rheumatoid arthritis (RA) N=112		Healthy control (HC) N=125		P-value	Odds ratio (OR) and confidence interval (CI)
	N	%	N	%
CC	93	83.0	117	93.6	0.0134*	0.3347, 0.1402–0.7988
CT	18	16.1	8	6.4	0.0216*	2.801, 1.166–6.726
TT	1	0.9	0	00	0.4726	3.377, 0.1361–83.80
Alleles
C	204	91.1	242	96.8	0.0104*	0.3372, 0.1454–0.7819
T	20	8.9	8	3.2		2.966, 1.279–6.877
Carriage rate
C	111	99.1	125	100	0.0254*	0.3739, 0.1574–0.8879
T	19	16.9	8	6.4		2.675, 1.126–6.352

Notes: N represents the number of individuals carrying a particular genotype in a study group; % represents the genotype frequency, allele frequency and carriage rates; *indicates significant P-values; χ² (P-value) indicates when HC is compared to RA.

Abbreviations: C, cytosine; T, thymine.

Figure 2 Representative gel picture of PTPN22 C<T polymorphism.

Notes: L1, DNA marker; 2, TT homozygous (171 bp, 41 bp); 3, heterozygous (215 bp, 174 bp); 4, CC homozygous (215 bp).
Abbreviations: C, cytosine; PTPN22, protein tyrosine phosphatase, non-receptor type 22 (lymphoid); T, thymine.

Table 2 Association of individual vitamin D receptor (VDR) FokI genotypes, alleles and their carriage rates

VDR genotype	Rheumatoid arthritis (RA) N=112	Healthy control (HC) N=125	P-value		Odds ratio (OR) and confidence interval (CI)
	N	%	N	%
FF	58	51.8	54	43.2	0.1952	1.412, 0.8458–2.358
Ff	50	44.7	63	50.4	0.4347	0.7937, 0.4758–1.324
ff	4	3.6	8	6.4	0.3898	0.5580, 0.1635–1.904
Alleles
F	166	74.1	171	68.4	0.1876	1.322, 0.8857–1.974
F	58	25.9	79	31.6		0.7563, 0.5066–1.129
Carriage rate
F	108	96.4	117	93.6	0.4339	1.214, 0.7815–1.885
F	54	48.2	71	56.8		0.8239, 0.5306–1.280

Notes: N represents the number of individuals carrying particular genotype in a study group; % represents the genotype frequency, allele frequency and carriage rates; χ² (P-value) indicates when HC is compared to RA.

Figure 3 Polymerase chain reaction (PCR)-based analysis of vitamin D receptor (VDR) gene Fok1 polymorphism.

Notes: 1, heterozygosity (265; 196 and 69 bp); 2 unexcisable fragment of (265 bp); 3, excisable fragment (196 bp and 69 bp); M, marker (50 bp ladder).