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International Journal of Nanomedicine Volume 10, 2015 - Issue
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Original Research

Antimicrobial activity and cytocompatibility of silver nanoparticles coated catheters via a biomimetic surface functionalization strategy

Ke Wu1 Department of Cardiology, The Affiliated Dongnan Hospital of Xiamen University, Zhangzhou, People’s Republic of China
,
Yun Yang2 Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People’s Republic of China;3 State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People’s Republic of China
,
Yanmei Zhang2 Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People’s Republic of China;3 State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People’s Republic of China
,
Jiexi Deng1 Department of Cardiology, The Affiliated Dongnan Hospital of Xiamen University, Zhangzhou, People’s Republic of ChinaCorrespondence[email protected]
&
Changjian Lin2 Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People’s Republic of China;3 State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People’s Republic of ChinaCorrespondence[email protected]
Pages 7241-7252 | Published online: 02 Dec 2015

Figures & data

Figure 1 SEM images of (A) CVC, (B) DCVC, (C and D) DCVC-Ag1, (E and F) DCVC-Ag2, and (G and H) DCVC-Ag3.

Abbreviations: CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

Figure 1 SEM images of (A) CVC, (B) DCVC, (C and D) DCVC-Ag1, (E and F) DCVC-Ag2, and (G and H) DCVC-Ag3.Abbreviations: CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

Table 1 Surface chemical composition and water contact angle of pristine CVC, DCVC, and DCVC-Ag samples (atomic percentage according to XPS analysis)

Figure 2 The survey XPS spectra of CVC, DCVC, and DCVC-Ag3.

Abbreviations: au, arbitrary unit; CVC, central venous catheter; DCVC, central ve nous catheters coated with polydopamine films; XPS, X-ray photoelectron spectroscopy.

Figure 2 The survey XPS spectra of CVC, DCVC, and DCVC-Ag3.Abbreviations: au, arbitrary unit; CVC, central venous catheter; DCVC, central ve nous catheters coated with polydopamine films; XPS, X-ray photoelectron spectroscopy.

Figure 3 The high-resolution XPS spectra of DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3.

Abbreviations: au, arbitrary unit; DCVC, central venous catheters coated with polydopamine films; XPS, X-ray photoelectron spectroscopy.

Figure 3 The high-resolution XPS spectra of DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3.Abbreviations: au, arbitrary unit; DCVC, central venous catheters coated with polydopamine films; XPS, X-ray photoelectron spectroscopy.

Figure 4 Raman spectra of various samples: (A) CVC, (B) DCVC, (C) DCVC-Ag3, (D) DCVC-Ag2, and (E) DCVC-Ag1.

Abbreviations: au, arbitrary unit; CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films.

Figure 4 Raman spectra of various samples: (A) CVC, (B) DCVC, (C) DCVC-Ag3, (D) DCVC-Ag2, and (E) DCVC-Ag1.Abbreviations: au, arbitrary unit; CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films.

Figure 5 Photographs showing the zone of inhibition assay for DCVC-Ag samples.

Abbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 5 Photographs showing the zone of inhibition assay for DCVC-Ag samples.Abbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 6 Fluorescent microscope images of Staphylococcus aureus stained on (A) DCVC, (B) DCVC-Ag1, (C) DCVC-Ag2, and (D) DCVC-Ag3 after 6 hours of culture.

Abbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 6 Fluorescent microscope images of Staphylococcus aureus stained on (A) DCVC, (B) DCVC-Ag1, (C) DCVC-Ag2, and (D) DCVC-Ag3 after 6 hours of culture.Abbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 7 WST-1 assay for proliferation of MC3T3-E1 cell on different samples at various incubation periods.

Note: Error bars represent mean ± SD (n=5), *P<0.05, **P<0.01.

Abbreviations: CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films; SD, standard deviation; WST-1, water soluble tetrazolium salts.

Figure 7 WST-1 assay for proliferation of MC3T3-E1 cell on different samples at various incubation periods.Note: Error bars represent mean ± SD (n=5), *P<0.05, **P<0.01.Abbreviations: CVC, central venous catheter; DCVC, central venous catheters coated with polydopamine films; SD, standard deviation; WST-1, water soluble tetrazolium salts.

Figure 8 Fluorescence microscopy images of Calcein-AM stained MC3T3-E1 cell attached on (A and B) DCVC, (C and D) DCVC-Ag1, (E and F) DCVC-Ag2, and (G and H) DCVC-Ag3 for 3 days.

Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.

Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

Abbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.
Figure 8 Fluorescence microscopy images of Calcein-AM stained MC3T3-E1 cell attached on (A and B) DCVC, (C and D) DCVC-Ag1, (E and F) DCVC-Ag2, and (G and H) DCVC-Ag3 for 3 days. Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.Display full sizeAbbreviation: DCVC, central venous catheters coated with polydopamine films.
Figure 8 Fluorescence microscopy images of Calcein-AM stained MC3T3-E1 cell attached on (A and B) DCVC, (C and D) DCVC-Ag1, (E and F) DCVC-Ag2, and (G and H) DCVC-Ag3 for 3 days. Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.Display full sizeAbbreviation: DCVC, central venous catheters coated with polydopamine films.

Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.

Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

Figure 9 The SEM images of MC3T3-E1 cell attachment on DCVC, DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 samples for days 3 and 7.Abbreviations: DCVC, central venous catheters coated with polydopamine films; SEM, scanning electron microscopy.

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