Association of TRPC1 Gene Polymorphisms with Type 2 Diabetes and Diabetic Nephropathy in Han Chinese Population

Figures & data

TABLE 1  Descriptive Characteristics of Study Population

				Sex
Group	N	Mean Age (SD)	P value^a	Male (%)	Female (%)	P value^a
Normal control	120	52.8 (6.4)		61 (50.8)	59 (49.2)
T2D with DN	134	59.3 (12.4)	<1 × 10^−3	75 (56.0)	59 (44.0)	0.413
T2D without DN	89	56.8 (11.8)	0.005	52 (58.4)	37 (41.6)	0.276
Combined T2D	223	58.3 (12.2)	<1 × 10^−3	127 (57.0)	96 (43.0)	0.278

Abbreviations: DN, diabetic nephropathy; SD, standard deviation; T2D, Type 2 diabetes mellitus.

^ap-Value in each group was calculated by comparing each T2D case group with normal control group.

TABLE 2  Genotype Distributions of the Six SNPs in Healthy Control and T2D Patients

		Genotype Frequencies				Hardy–Weinberg Proportions (p)^a
SNP rsID	Genotype	Control	Combined T2D	T2D with DN	T2D without DN	Control	Combined T2D	T2D with DN	T2D without DN
rs953239	A/A	62 (52.5%)	107 (48%)	71 (53%)	36 (40.5%)
	C/A	45 (38.1%)	102 (45.7%)	57 (42.5%)	45 (50.6%)	0.5	0.14	0.26	0.35
	C/C	11 (9.3%)	14 (6.3%)	6 (4.5%)	8 (9%)
rs7638459	T/T	42 (35.3%)	52 (26%)	34 (28.3%)	18 (22.5%)
	C/T	58 (48.7%)	103 (51.5%)	61 (50.8%)	42 (52.5%)	1	0.78	0.86	0.82
	C/C	19 (16%)	45 (22.5%)	25 (20.8%)	20 (25%)
rs7621642	G/G	46 (38.3%)	72 (32.6%)	45 (34.1%)	27 (30.3%)
	G/A	56 (46.7%)	119 (53.9%)	71 (53.8%)	48 (53.9%)	0.85	0.095	0.2	0.39
	A/A	18 (15%)	30 (13.6%)	16 (12.1%)	14 (15.7%)
rs2033912	A/A	40 (33.3%)	79 (35.4%)	50 (37.3%)	29 (32.6%)
	T/A	63 (52.5%)	113 (50.7%)	68 (50.8%)	45 (50.6%)	0.45	0.4	0.36	0.83
	T/T	17 (14.2%)	31 (13.9%)	16 (11.9%)	15 (16.9%)
rs3821647	G/G	67 (57.3%)	125 (56.3%)	79 (59%)	46 (52.3%)
	A/G	43 (36.8%)	86 (38.7%)	49 (36.6%)	37 (42%)	1	0.58	0.81	0.59
	A/A	7 (6%)	11 (5%)	6 (4.5%)	5 (5.7%)
rs7610200	G/G	53 (44.2%)	81 (42%)	47 (41.2%)	34 (43%)
	A/G	52 (43.3%)	92 (47.7%)	57 (50%)	35 (44.3%)	0.69	0.52	0.29	0.81
	A/A	15 (12.5%)	20 (10.4%)	10 (8.8%)	10 (12.7%)

Abbreviations: DN, diabetic nephropathy; SNP, single nucleotide polymorphism; T2D, Type 2 diabetes mellitus.

^ap-Values for differences between T2D patients and healthy control by χ² analysis.

TABLE 3  Allele Frequencies Distributions and Minor Allele Frequencies of TRPC1 Polymorthisms

		Allele Frequencies					MAF
dbSNP rsID	SNP type	Control	Combined T2D	T2D with DN	T2D without DN	Control	Combined T2D	T2D with DN	T2D without DN
rs953239	A	169 (0.72)	316 (0.71)	199 (0.74)	117 (0.66)	C 0.28	C 0.29	C 0.26	C 0.34
	C	67 (0.28)	130 (0.29)	69 (0.26)	61 (0.34)
rs7638459	T	142 (0.6)	207 (0.52)	129 (0.54)	78 (0.49)	C 0.4	C 0.48	C 0.46	T 0.49
	C	96 (0.4)	193 (0.48)	111 (0.46)	82 (0.51)
rs7621642	G	148 (0.62)	263 (0.6)	161 (0.61)	102 (0.57)	A 0.38	A 0.4	A 0.39	A 0.43
	A	92 (0.38)	179 (0.4)	103 (0.39)	76 (0.43)
rs2033912	A	143 (0.6)	271 (0.61)	168 (0.63)	103 (0.58)	T 0.4	T 0.39	T 0.37	T 0.42
	T	97 (0.4)	175 (0.39)	100 (0.37)	75 (0.42)
rs3821647	G	177 (0.76)	336 (0.76)	207 (0.77)	129 (0.73)	A 0.24	A 0.24	A 0.23	A 0.27
	A	57 (0.24)	108 (0.24)	61 (0.23)	47 (0.27)
rs7610200	G	158 (0.66)	254 (0.66)	151 (0.66)	103 (0.65)	A 0.34	A 0.34	A 0.34	A 0.35
	A	82 (0.34)	132 (0.34)	7 (0.34)	5 (0.35)

Abbreviations: DN, diabetic nephropathy; SNP, single nucleotide polymorphism; T2D, Type 2 diabetes mellitus.

TABLE 4  rs7638459 Association with Response Status in Healthy Control and in T2D Patients without DN (Adjusted by Gender and Age)

Model Type	Genotype	Control (%)	T2D without DN (%)	OR (95% CI)	p^a
Codominant	T/T	42 (35.3%)	18 (22.8%)	1.00	0.13
	C/T	58 (48.7%)	42 (53.2%)	1.58 (0.79–3.19)
	C/C	19 (16%)	19 (24.1%)	2.39 (1.00–5.68)
Dominant	T/T	42 (35.3%)	18 (22.8%)	1.00	0.086
	C/T-C/C	77 (64.7%)	61 (77.2%)	1.77 (0.91–3.45)
Recessive	T/T-C/T	100 (84%)	60 (76%)	1.00	0.13
	C/C	19 (16%)	19 (24.1%)	1.78 (0.85–3.71)
Overdominant	T/T-C/C	61 (51.3%)	37 (46.8%)	1.00	0.73
	C/T	58 (48.7%)	42 (53.2%)	1.11 (0.62–1.99)
Log-additive	–	–	–	1.55 (1.01–2.38)	0.045

Abbreviations: DN, diabetic nephropathy; SNP, single nucleotide polymorphism; T2D, Type 2 diabetes mellitus; OR, Odds ratio; 95% CI, 95% confidence interval.

The OR values were counted by the web-based tool SNPstats, adjusted by gender and age.

^ap-Values for the differences between controls and T2D without DN in every chosen model, counted by the web-based tool SNPstats.

*p < 0.05

TABLE 5  rs953239 Association with Response Status in T2D Patients without DN and in T2D Patients with DN (Adjusted by Gender and Age)

Model Type	Genotype	T2D without DN (%)	T2D with DN (%)	OR (95% CI)	^ap
Codominant	A/A	36 (40.9%)	71 (53%)	1.00	0.13
	C/A	44 (50%)	57 (42.5%)	0.65 (0.37–1.15)
	C/C	8 (9.1%)	6 (4.5%)	0.37 (0.12–1.16)
Dominant	A/A	36 (40.9%)	71 (53%)	1.00	0.075
	C/A-C/C	52 (59.1%)	63 (47%)	0.61 (0.35–1.05)
Recessive	A/A-C/A	80 (90.9%)	128 (95.5%)	1.00	0.16
	C/C	8 (9.1%)	6 (4.5%)	0.46 (0.15–1.38)
Overdominant	A/A-C/C	44 (50%)	77 (57.5%)	1.00	0.27
	C/A	44 (50%)	57 (42.5%)	0.74 (0.43–1.27)
Log-additive	–	–	–	0.63 (0.40–0.99)	0.043

Abbreviations: DN, diabetic nephropathy; T2D, Type 2 diabetes mellitus; OR, Odds ratio; 95% CI, 95% confidence interval.

^ap-Values for the differences between T2D without DN and T2D with DN in every chosen model, counted by the web-based tool SNPstats.

*p < 0.05.