Cerebroprotective effect of Ocimum gratissimum against focal ischemia and reperfusion-induced cerebral injury

Figures & data

Table 1.  Quantitative analyses of phenolic content of O. gratissimum ethanol extract (EEOg).

RA (% w/w)	pCA (% w/w)	HBA (% w/w)	FA (% w/w)	CA (% w/w)	LA (% w/w)
0.025 ± 0.01	0.019 ± 0.03	0.012 ± 0.04	0.015 ± 0.01	0.006 ± 0.03	0.010 ± 0.02

RA, rosmarinic acid; pCA, p-Coumaric acid; HBA, Hydroxy benzoic acid; FA, Ferulic acid; CA, Cinnamic acid; LA, Lithospermic acid.

Figure 1.  A) Neuroprotective effect of ethanol extract of O. gratissimum (EEOg) on brain infarction in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Tukey’s post hoc multiple range test; *p <0.05 versus control; #p < 0.05 versus sham; ^ap <0.05 versus 150 mg/kg dose of EEOg. B) Effect of EEOg on impairment of short-term memory (plus maze test) in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Tukey’s post hoc multiple range test; *p <0.05 versus control; #p < 0.05 versus sham; ^ap <0.05 versus 150 mg/kg dose of EEOg.

Figure 2.  Effect of ethanol extract of O. gratissimum (EEOg) on thiobarbituric acid reactive substances (TBARS) formation in brain of rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Tukey’s post hoc multiple range test; #p <0.05 versus sham; *p <0.05 versus control; ^ap <0.05 versus 150 mg/kg dose of EEOg.

Figure 3.  A) Effect of ethanol extract of O. gratissimum (EEOg) on superoxide dismutase activity in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Tukey’s post hoc multiple range test; *p <0.05 versus control; #p <0.05 versus sham; ^ap <0.05 versus 150 mg/kg dose of EEOg. B) Effect of EEOg on glutathione peroxidase (GPx) activity in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Tukey’s post hoc multiple range test; *p <0.05 versus control; #p <0.05 versus sham; ^ap <0.05 versus 150 mg/kg dose of EEOg.

Figure 4.  A) Effect of ethanol extract of O. gratissimum (EEOg) on impairment of motor performance (inclined beam test) in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± S.D.; n = 7; ANOVA followed by post hoc Kruskal-Wallis test; #p <0.05 versus sham; *p <0.05 versus control; ^ap <0.05 versus 150 mg/kg dose of EEOg. B) Effect of EEOg on impairment of motor performance (rota-rod test) in rats subjected to focal cerebral ischemia and 24 h reperfusion injury. The data are expressed as mean ± SD; n = 7; ANOVA followed by Bonferroni’s post hoc correction test; *p <0.05 versus control; #p <0.05 versus sham.