Conferin, potent antioxidant and anti-inflammatory isoflavone from Caragana conferta Benth

Figures & data

Figure 1.  Compounds 1 and 2.

Figure 2.  HMBC & NOESY correlations for compound 1.

Table 1.  IC₅₀ (μM) values of compounds 1 and 2 in antioxidant assay and against lipoxygenase.

Compound	DPPH scavenging activity, IC50^a (μM)	Lipoxygenase inhibition activity, IC50^a (μM)
1	19.1 ± 0.11	55.2 ± 0.22
2	99.5 ± 0.15	100 ± 0.12
BHA^b	44.3 ± 0.09
Baicalein^c		22.6 ± 0.08

^aValue ± SEM (standard mean error of three assays).

^bStandard DPPH scavenging activity.

^cStandard inhibitor of lipoxygenase.

Figure 3.  Reducing ability of BHA and compounds 1 and 2 at various concentrations.

Table 2.  Anti-inflammatory potential of compounds 1 and 2 in carrageenan-induced paw edema of rats.

Sr no.	Treatment (100 mg/kg)	Initial paw volume^a, Vo	Final volume^a, Vf (2–3 h)	Edema volume^a, Vf – Vo = Vc	Percent inhibition
1	Cage 1, control	0.86 ± 0.08	0.86 ± 0.09	0	—
2	Diclofenac sodium	0.83 ± 0.09	1.04 ± 0.15	0.21 ± 0.23	58
3	1	0.93 ± 0.07	1.13 ± 0.05	0.24 ± 0.15	52
4	2	0.92 ± 0.13	1.19 ± 0.05	0.27 ± 0.21	54

^aMeasured in mL.