Apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and kit

Figures & data

Figure 1.  Chemical structures of standard antioxidants (BHA, BHT, α-tocopherol and trolox), majdine and isomajdine from Vinca herbacea Waldst. and Kit.

Figure 2.  (A) Fe³⁺-Fe²⁺ reductive potential of different concentrations (10–30 g/mL) of majdine (r²:0.933), isomajdine (r²:0.933) and reference antioxidants BHA, BHT, α-tocopherol and trolox using spectrophotometric detection of the Fe⁺³-Fe⁺² transformation. (B) Cupric ions (Cu²⁺) reducing ability (Cuprac method) of different concentrations (10–30 g/mL) of majdine (r²: 0.9330), isomajdine (r²: 0.9330), BHA, BHT, α-tocopherol and trolox using spectrophotometric detection of the Cu⁺²-Cu⁺ transformation. (BHA: Butylated hydroxyanisole, BHT: Butylated hydroxytoluene).

Table 1.  Comparison of IC₅₀ values of ABTS^•+ scavenging, DMPD^•+ scavenging and ferrous ion (Fe²⁺) chelating activity of majdine, isomajdine and standard antioxidant compounds such as BHA, BHT, α-tocopherol and trolox (BHA: butylated hydroxyanisole, BHT: butylated hydroxytoluene, ABTS^•+:2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals, DMPD^•+: N,N-dimethyl-p-phenylenediamine radicals).

Compounds	ABTS^•+ scavenging	DMPD^•+ scavenging	Metal chelating
BHA	4.65	63.25	34.31
BHT	52.81	—*	13.72
Trolox	62.32	14.89	16.17
α-Tocopherol	73.15	—*	16.31
Isomajdine	34.11	32.09	10.05
Majdine	57.69	71.24	14.01

*It was reported the main drawback of the DMPD^•+ method is that its sensitivity and reproducibility dramatically decreased when hydrophobic antioxidants such as α-tocopherol or BHT were used. Hence, these standard antioxidant compounds were not used in this antiradical assay^28,37.

Figure 3.  WST-1 assay of the groups. [H₂O₂: 1 mM of hydrogen peroxide. Iso: isomajdine and majd: majdine in µM concentrations. *p < 0.05, **p < 0.01 with respect to control group (Con), WST-1: water-soluble tetrazolium salt].