Isolation of a new bioactive cinnamic acid derivative from the whole plant of Viola betonicifolia

Figures & data

Table 1.  ¹H-NMR and ¹³C-NMR chemical shift values of 1.

C. No.	δC	δH (J, Hz)
1	112.5	–
2	151.5	–
3	103.9	6.77 s
4	153.0	–
5	147.1	–
6	109.9	7.11 s
7	146.2	7.85 d (9.5)
8	112.6	6.20 d (9.5)
9	164.1	–
5-OMe	56.8	3.90 s
2-OH	–	5.44 s
4-OH	–	5.23 s

Figure 1.  Structure of compound 1 (2,4-dihydroxy, 5-methoxy-cinnamic acid, trans).

Table 2.  Effect of compound 1 and standard drugs in DPPH free radical scavenging and antiglycation assays.

Drugs	Activity in terms of IC50 (µM)
	DPPH free radicals	Antiglycation assay
Compound 1	124 ± 5.76 µM	−
α-tocopherole	96 ± 0.46	−
Vitamin C	90 ± 0.56	−
Rutin	−	294 ± 0.56

Data represent mean ± SEM of three independent assays.

DPPH, diphenyl-2-picryl hydrazyl.