Figures & data
Figure 1. Isolation of sesquiterpenoids (compounds 1–4) from Tussilago farfara. (A) The structures of sesquiterpenoids isolated from Tussilago farfara. (B) NO was measured in LPS-treated BV-2 cells after treatment of test compounds from Tussilago farfara. **p < 0.01 compared with LPS-treated group (med: media).
Figure 2. The inhibitory effects of sesquiterpenoids (compounds 1–4) on LPS-induced production of cytotoxic factors in BV-2 cell. (A) TNF-α was measured by the ELISA after treatment of test compounds in BV-2 cells. (B) PGE2 was measured by ELISA with the treatment of test compounds in LPS-activated BV-2 cells. (C) ROS generation was measured by FACS after treatment of test compounds in BV-2 cells. (D) BV-2 cell viability was measured by MTT after treatment of test compounds (10 μM) for 24 h. Values are mean ± SD of three experiments. **p < 0.01 compared with LPS-treated group (med: media).
Figure 3. The inhibitory effects of sesquiterpenoids (compounds 1–4) on the expression of iNOS and COX-2 in LPS-activated BV-2 cells. (A) The protein level of iNOS and COX-2 were measured by Western blotting. (B) The mRNA level of iNOS and COX-2 were measured by RT-PCR. (C) The protein level of Iκ-B was measured by Western blotting.
Figure 4. The inhibitory effect of sesquiterpenoids (compounds 1–4) on microglia-induced neuronal cell death. Cell viability of PC12 cells was measured by MTT assay after treatment of conditioned media from BV-2 cells prepared as described in material and methods. The viability was represented as the fold of media control of PC12 cells. **p < 0.01 compared with LPS-treated group.
